We narrowed to 1,265 results for: ncl
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Plasmid#103805PurposeBLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorExpressionMammalianMutationhNCL: A395G (K132R), A421G (K141E), A424G (K142E)…PromoterCMVAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only
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pCIBN-EYFP-hNCL
Plasmid#103806PurposeBLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorExpressionMammalianMutationhNCL: A395G (K132R), A421G (K141E), A424G (K142E)…PromoterCMVAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL-p2x-LanCL2
Plasmid#154192PurposeTo express MBP-LanCL2 in bacterial cellsDepositorInsertLanCL2
TagsMaltose binding protein (MBP)ExpressionBacterialAvailable SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EGFP.T2A.NCLX.3'UTR
Plasmid#181872PurposeExpresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter (includes a large portion of the Slc8b1 3'UTR)DepositorInsertsUseAAV and AdenoviralTagsHA, T2A, and mycExpressionMammalianMutationincludes a large portion of the Slc8b1 3'UTR…Promotersynthetic hybrid CAG promoterAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1-WT-LanCL2-C-Ter-His
Plasmid#154186PurposeTo express LanCL2 in bacterial cellsDepositorInsertLanCL2
TagsHexahistidineExpressionBacterialAvailable SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pML384_NCLN-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227897PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertNCLN homology arms with mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NCL-gRNA1-EGFP
Plasmid#226004PurposeCRISPRi-KD of human NucleolinDepositorInsertsgRNA targeting human Nucleolin (NCL Human)
ExpressionMammalianAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NCL-gRNA2-EGFP
Plasmid#226005PurposeCRISPRi-KD of human NucleolinDepositorInsertsgRNA targeting human Nucleolin (NCL Human)
ExpressionMammalianAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI-SMN2-inclusion-GLuc
Plasmid#218671PurposeAlternative splicing signaling: signal increased with Ex7 inclusion increasingDepositorInsertSMN2 (SMN2 )
ExpressionMammalianAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-LanCL1-H219A
Plasmid#154190PurposeTo express LanCL1-H219A in bacterial cellsDepositorInsertLanCL1
TagsHexahistidineExpressionBacterialMutationHis219->Ala219Available SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only