We narrowed to 63 results for: Toxoplasma gondii
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Plasmid#25194PurposeBacterial expression for structure determination; may not be full ORFDepositorInsert38.m00014:T112-A378
TagsHisExpressionBacterialMutationContains amino acids T112-A378Available SinceAug. 9, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
PP-CDPK1 (3DXN)
Plasmid#25129PurposeBacterial expression for structure determination; may not be full ORFDepositorInsert541.m00134:MAC02T-C05:C40091
TagsHisExpressionBacterialMutationContains amino acids L72-Q339Available SinceAug. 5, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
PP-MAPK2 (3RP9)
Plasmid#32862PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMAPK2
TagsHis tag with TEV cleavageExpressionBacterialMutationamino acids 123-548 only, P421LAvailable SinceJan. 10, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGCaMP5
Plasmid#78705PurposeGCaMP5 under the Toxoplasma SAG1 5' UTR. No Toxoplasma selectable markerDepositorInsertGCaMP5
PromoterSAG1 5' UTRAvailable SinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
3HX4
Plasmid#60137PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTgCDPK4:MAC03F-A01:C208088
Tags6xHis and TEV cleavage siteExpressionBacterialMutationSee commentsPromoterT7Available SinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-UPRT
Plasmid#69310Purposeexpresses UPRT cDNADepositorInsertUPRT cDNA
TagsHAExpressionBacterialAvailable SinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-HA::UPRT
Plasmid#47110Purposefor subcloning UPRT into expression vectorsDepositorInserturacil phosphoribosyltransferase
TagsHAAvailable SinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTet-On-TAP
Plasmid#59017PurposeConstruct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.DepositorTypeEmpty backboneUseToxoplasma expressionTagsCalmodulin Binding Peptide fused with a TEV Cleav…PromoterRPS13 with Tet Operators InsertedAvailable SinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMVtight-UPRT-T2A-RFP-IRES-CD
Plasmid#126677PurposeDoxycycline inducible expression of UPRT and CD along with reporter RFPDepositorInsertUPRT-T2A-RFP-IRES-CD (FCY1 Toxoplasma gondii, Budding Yeast)
Promotertight TREAvailable SinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-Tagger
Plasmid#129396PurposeExpresses 2A-separated rpl22, UPRT, mKate2 and Ago2 driven by CMV promoterDepositorInsertrpl22-HA-2A-UPRT-mKate2-FLAG-V5-Ago2
TagsFLAG, HA, and V5ExpressionMammalianPromoterCMVAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDiCre-mCherry
Plasmid#164572PurposeIntegration of a DiCre cassette in PbGFP parasite genomeDepositorInsertsFKBP12-CRE59
FRB-CRE60
mCherry
Bifunctional dihydrofolate reductase-thymidylate synthase (TGME49_249180 Toxoplasma gondii)
UseCre/LoxPromoterBidirectional PbEF1alpha promoter (PBANKA_1133300…Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-SAG1-DHFR
Plasmid#80322PurposeEncodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertSAG1 sgRNA
UseCRISPRPromoterU6Available SinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6-Decoy
Plasmid#80324PurposeEncodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondiiDepositorInsertDecoy sgRNA
UseCRISPRPromoterU6Available SinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only