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pDMJ2-SARS-CoV-2-Spike (XBB-5b)

Plasmid

#241126

Purpose

Expresses SARS-CoV-2 spike (XBB-5b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (XBB-5b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,T76I,V83A,G142D,Y1…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Muc5b D1 (murine) with cleavable His tag

Plasmid

#181814

Purpose

Expresses murine Muc5b D1 with cleavable His tag, residues 50-426

Depositor

Deborah Fass

Article

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

Muc5b D1 (Muc5b Mouse)

Tags

TEV cleavage site followed by 6xHistidine tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.5-5b)

Plasmid

#241108

Purpose

Expresses SARS-CoV-2 spike (BA.5-5b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.5-5b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

N481T,T76I,M153S,Y473F,Q493R,T19I,L24del,P25del,P…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.75-5b)

Plasmid

#241082

Purpose

Expresses SARS-CoV-2 spike (BA.2.75-5b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.75-5b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,G142D,K147N,W152R,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pBS Wnt-5bHA

Plasmid

#17377

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-5bHA (Wnt5b Mouse)

Tags

HA

Expression

Bacterial

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pL0-NbPT5bPro

Plasmid

#159416

Purpose

Nicotiana benthamiana PT5b promoter sequence (1068 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

Depositor

Sam Brockington

Article

Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

Insert

NbPT5b Promoter

Use

Puc19-derived

Expression

Bacterial

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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Reckleen_3plus_sgRNA_ptet_pos5b

Plasmid

#233464

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pX330GFP-hU6-gRNA-hSpCas9

Plasmid

#128385

Purpose

GFP positive gRNA/hSpCas9 construct

Depositor

Budd A. Tucker

Article

Giacalone et al Curr Protoc Stem Cell Biol. 2018 Feb 28;

Insert

GFP, gRNA and hSpCas9

Use

CRISPR

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET-17b_K490clm_E215C

Plasmid

#169995

Purpose

Kinesin KIF5B K490 cysteine light mutant with E215C mutation for single cysteine labeling

Depositor

Ron Vale

Article

Niekamp et al Proc Natl Acad Sci U S A. 2019 Mar 5;116

Insert

kinesin family member 5B, isoform CRA_a

Tags

6xHIS

Expression

Bacterial

Mutation

C7S, C65A, C168A, C174S, E215C, C294A, C330S, C42…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pBSc-twk31

Plasmid

#16126

Depositor

Larry Salkoff

Article

Salkoff et al Neuroscience. 2001 . 103(4):853-9.

Insert

Tandem pore domain K+ channel

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pAD-CMV-STAT5B-DN-CMV-GFP

Plasmid

#83262

Purpose

Adenoviral expression of dominant negative STAT5B with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5b (Stat5b Mouse)
eGFP

Use

Adenoviral

Tags

FLAG

Mutation

dominant negative (Y699F)

Promoter

CMV

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pFLAG-TNKS-2-M1054V

Plasmid

#34692

Depositor

Nai-Wen Chi

Article

Sbodio et al Biochem J. 2002 Feb 1;361(Pt 3):451-9.

Insert

TNKS2-M1054V (TNKS2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

M1054V

Promoter

CMV

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pLV-Kif5b-Myc

Plasmid

#186614

Purpose

Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform B fused to Myc tag

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Heavy Chain isoform 5b (KIF5B Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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SF4413

Plasmid

#255513

Purpose

DERA enzyme production under control of T7 promoter and inducible lac operator

Depositor

Radhakrishnan Mahadevan

Article

Kim et al J Biol Chem. 2020 Jan 10;295(2):597-609.

Insert

2-deoxyribose-5-phosphate aldolase (DERA) from Shigella flexneri serotype 5b

Tags

6xHis tag-TEV protease recognition site (ENLYFQ/G…

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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pGEMHE-TNKS-2

Plasmid

#154149

Purpose

For making TNKS-2 RNA

Depositor

Carmen Williams

Article

Gambini et al Dev Cell. 2020 Jun 8;53(5):545-560.e7. d

Insert

Tankyrase 2 (TNKS2 Human)

Use

In vitro transcription

Tags

FLAG

Promoter

T7

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGEMHE-TNKS-2-M1054V

Plasmid

#138538

Purpose

For making TNKS-2-M1054V RNA

Depositor

Carmen Williams

Insert

Tankyrase 2 (TNKS2 Human)

Use

In vitro transcription

Tags

FLAG

Mutation

Changed methionine 1054 to valine

Promoter

T7

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAG195 TNKS2 3'UTR

Plasmid

#11999

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

TNKS2 3'UTR (TNKS2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pLV-Kif5b-T92N-Myc

Plasmid

#186615

Purpose

Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform B Rigor mutant (T92N) fused to Myc tag

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Heavy Chain isoform 5b Rigor mutant (T92N) (KIF5B Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Mutation

T92N

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAG228 TNKS2 3'UTR mut

Plasmid

#12031

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

TNKS2 3'UTR mut (TNKS2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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TNKS2A

Plasmid

#42463

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

TNKS2A (TNKS2 Human)

Tags

His6

Expression

Bacterial

Mutation

See Comments

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only