-
Plasmid#164918PurposeCEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNADepositorInsertP(Z)-mcherry
UseCRISPRTagsExpressionYeastMutationPromoterP(Z), synthetic promoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUb-HisUb-Ubc9-ChE1 v4.5
Plasmid#185756PurposeBacterial expression of His-Ub, Ubc9 and ChE1 v4.5DepositorInsertsHis-Ub
Ubc9
ChE1 v4.5
UseTagsHis6ExpressionMutationK14RPromoterT7Available sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSC-PreE2
Plasmid#52264PurposeBacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linkerDepositorInsertUbc9 GST fusion (UBE2I Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSC-IntE2
Plasmid#52265PurposeBacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with GyrA intein linkerDepositorInsertUbc9 GST fusion (UBE2I Human, Synthetic)
UseTagsGST and GyrA-inteinExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
pET28a-RANGAP1-CTD
Plasmid#228947Purposeexpress the C-terminal domain of the human RANGAP1 protein (residues 418-587 of UniProt P46060 with the N-terminal thrombin-cleavable MGSSHHHHHHSSGLVPR|GSHM- tag). Codon-optimizedDepositorInsertRANGAP1-CTD (RANGAP1 Human)
UseTagsHis tag and thrombine cleavage site : MGSSHHHHHHS…ExpressionBacterialMutationcontains aa 418-587 onlyPromoterT7-lacAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-ChE1 v4.5
Plasmid#185755PurposeBacterial expression of ChE1 v4.5DepositorInsertChE1 v4.5
UseTagsHis10ExpressionBacterialMutationPromoterT7Available sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-shortSUMO1
Plasmid#228951Purposeexpress a fragment of the human SUMO1 protein (residues 18-97 of UniProt P6316 with an N-terminal thrombin-cleavable tag, MGSSHHHHHHSSGLVPR|GSHM-, where | indicates the cleavage site). Codon-optimizedDepositorInsertSUMO1 (SUMO1 Human)
UseTagsHis tag and thrombine cleavage site : MGSSHHHHHHS…ExpressionBacterialMutationdeleted amino acids 1-17PromoterT7-lacAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
p3991 pET ubcH9
Plasmid#8651DepositorInsertUbcH9 (UBE2I Human)
UseTagsHAExpressionBacterialMutationPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
-
pET11a-hRanGAP1
Plasmid#50941Purposeexpression of full length untagged human RanGAP1 in E.coliDepositorInsertRanGTPase activating protein 1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
RanBP2-RB3-4-pET23a
Plasmid#85769Purposebacterial expression of RanBP2 SUMO E3 ligase fragment (aa 2304-3062) with C-terminal His tagDepositorInsertRanBP2 fragment (RANBP2 Human)
UseTagsHisExpressionBacterialMutationfragment (amino acids 2304-3062)PromoterT7Available sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSub-MBP-GFP(2M-IKQE)
Plasmid#185757PurposeBacterial expression of MBP-GFP(2M IKQE)DepositorInsertGFP(2M-IKQE)
UseTagsMBPExpressionBacterialMutationPromotertacAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-E2
Plasmid#175242PurposeBacterial expression and purification, E2 enzyme (UBC9) for SUMOylationDepositorInsertUBE2I (UBE2I Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSub-Asp6-MBP-HRas
Plasmid#185758PurposeBacterial expression of Asp6-MBP-HRasDepositorInsertAsp6-MBP-HRas
UseTagsMBPExpressionBacterialMutation6x Asp added to the N terminus of MBPPromotertacAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
CyPet-FKBP-E2
Plasmid#175244PurposeBacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycinDepositorInsertUBE2I (UBE2I Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUMO1-del
Plasmid#228950Purposeexpress the full-length human SAE1:SAE2 heterodimer ( where SAE1 is untagged and SAE2 contains an N-terminal tag : MGSSHHHHHHSQDADLNSRVD, derivated from the Addgene plasmid #52258 pSUMO1)DepositorUseTagsHis tag and thrombine cleavage site : MGSSHHHHHHS…ExpressionBacterialMutationPromoterRBS and T7-lacAvailable sinceMay 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PML RB1
Plasmid#185636Purposeexpression of GST-tagged PML fragment 49-168 (RING + B-box1) in bacterial cellsDepositorInsertpromyelocytic leukemia protein (PML Human)
UseTagsGSTExpressionBacterialMutationnonePromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/T0-3xFLAG-NFATC2IP D394R
Plasmid#221211PurposeMammalian expression vector of triple Flag tagged NFATC2IP ORF with D394R point mutation. Charge-reverting mutation for UBC9 R13D mutant for interactionDepositorInsertNFATC2IP (NFATC2IP Human)
UseTags3XFlagExpressionMammalianMutationD394R (charge-reverting mutation for interaction …PromoterCMVAvailable sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT/T0-eGFP-NFATC2IP D394R
Plasmid#221436PurposeMammalian expression vector of eGFP tagged NFATC2IP ORF with D394R point mutation. Charge-reverting mutation for UBC9 R13D mutant for interactionDepositorInsertNFATC2IP (NFATC2IP Human)
UseTagseGFPExpressionMammalianMutationD394R (charge-reverting mutation for interaction …PromoterCMVAvailable sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits