Addgene

We narrowed to 485 results for: transposase

Showing: 21 - 40 of 485 results

Results per page:

Sort results by:

pXT130_TwinStrep-SUMO-ShTnsC

Plasmid

#135526

Purpose

Protein expression construct for ShTnsC

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTnsC

Use

Tags

TwinStrep-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pXT131_TwinStrep-SUMO-ShTniQ

Plasmid

#135527

Purpose

Protein expression construct for ShTniQ

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTniQ

Use

Tags

TwinStrep-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pXT129_TwinStrep-SUMO-ShTnsB

Plasmid

#135525

Purpose

Protein expression construct for ShTnsB

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTnsB

Use

Tags

TwinStrep-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDonor_AcCAST_kanR

Plasmid

#127925

Purpose

Kanamycin resistance donor for AcCAST

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

KanR

Use

Tags

Expression

Mutation

Promoter

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRO16Sh2

Plasmid

#180604

Purpose

Expression of ShCAST under control of Plac with pRO1600 origin.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLac

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRO16Sh5

Plasmid

#180076

Purpose

Expression of ShCAST under control of Plac with pRO1600 origin.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLac

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJY4092

Plasmid

#113334

Purpose

Vector for Hermes transposition; Hermes TIR-flanked AvrII cut site; recoded transposase driven by TEF1 promoter

Depositor

Suzanne Sandmeyer

Insert

codon-optimized Hermes transposase

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pXT128_TwinStrep-SUMO-ShCas12k

Plasmid

#135524

Purpose

Protein expression construct for ShCast12k

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShCas12k

Use

Tags

TwinStrep-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBLlSh2

Plasmid

#170981

Purpose

Expression of ShCAST under control of lacI Plac promoter/repressor pair.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Inserts

lacI
ShTnsB, ShTnsC, ShTniQ, ShCas12k

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlac01

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
VR122 pETv2 10His-pG-Tn5 (E54K, L372P)

Plasmid

#198467

Purpose

E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.

Depositor

Viviana Risca

Article

Soroczynski et al bioRxiv 2024.07.11.602973

Insert

10His-pG-Tn5 (E54K, L372P)

Use

Tags

10His-pG

Expression

Bacterial

Mutation

E54K, L372P

Promoter

Consensus φ10 T7 Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDonor14-AcHELIX-KanR (CJT96)

Plasmid

#181794

Purpose

pDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTXB1-Tn5

Plasmid

#60240

Purpose

IPTG-inducible expression of Transposon Tn5 fused to Mxe Intein and Chitin-binding domain.

Depositor

Rickard Sandberg

Article

Picelli et al Genome Res. 2014 Jul 30. pii: gr.177881.

Insert

tnpA from pBam1

Use

Tags

Mxe intein - Chitin-binding domain

Expression

Bacterial

Mutation

E54K, L372P

Promoter

T7 promoter

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pN7HELIX

Plasmid

#190662

Purpose

Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB

Use

Tags

Expression

Mammalian

Mutation

nAniI = K227M, F80K, L232K

Promoter

CMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pN7CAST

Plasmid

#190661

Purpose

Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-HIS-Tol2

Plasmid

#112486

Purpose

functional recombinant Tol2 protein from E.coli

Depositor

Stephen Ekker

Article

Ni et al Mob DNA. 2016 Mar 31;7:6. doi: 10.1186/s

Insert

Tol2 Transposase (tpase Synthetic)

Use

Tags

HIS/6x

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDonor14-ShoHELIX-KanR (BO4)

Plasmid

#181795

Purpose

pDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCasTns(Ptr)-IS186

Plasmid

#175581

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(IS186)

Use

Tags

Expression

Bacterial

Mutation

NO

Promoter

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcCAST-sgRNA_entry (CJT83)

Plasmid

#181785

Purpose

Expresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

AcTnsB, AcTnsC, AcTniQ, AcCas12k

Use

Tags

Expression

Bacterial

Mutation

Promoter

Lac and J23119

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDonor-LoxP

Plasmid

#219436

Purpose

CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens

Depositor

Sheng Yang

Article

Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

Insert

two transposon ends, LoxP-terminator-LoxP gene cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pShoCAST-sgRNA_entry (BO1)

Plasmid

#181786

Purpose

Expresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k

Use

Tags

Expression

Bacterial

Mutation

Promoter

Lac and J23119

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge