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Showing: 381 - 400 of 12605 results

  7. Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)

    Plasmid
    #208944
    Purpose
    A control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    dSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208944-plasmid-map-sequence-id-415672

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  9. Control click editor - pCMV-T7-deadPCV2-nCas9-EcKlenow (EMK492)

    Plasmid
    #208943
    Purpose
    A control CE1 construct with catalytically attenuated PCV2 HUH, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    dPCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    PCV2(Y96F); nSpCas9(H840A); EcKlenow(-exo;D355A/E…
    Promoter
    CMV and T7
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208943-plasmid-map-sequence-id-415668

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  10. pET MBP mRuby2 LIC cloning vector (MBP-mRuby2)

    Plasmid
    #86933
    Purpose
    pET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mRuby2
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86933-plasmid-map-sequence-id-174748

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  11. Polymerase-recruited click editor - pCMV-T7-PCV2-nCas9-CC(N6) (LM2705)

    Plasmid
    #208962
    Purpose
    Polymerase-recruited click editor comprised of PCV2 fused to nCas9 with a C-terminal N6 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-CC(N6)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208962-plasmid-map-sequence-id-415796

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  12. Control click editor - pCMV-T7-PCV2-nCas9-deadEcKlenow (JO520)

    Plasmid
    #208945
    Purpose
    A control CE1 construct with catalytically attenuated EcKlenow DNA polymerase, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-dEcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A);deadEcKlenow(-exo;D355A,D357A,D705…
    Promoter
    CMV and T7
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208945-plasmid-map-sequence-id-415676

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  14. pET MBP mClover LIC cloning vector (MBP-mClover)

    Plasmid
    #86930
    Purpose
    pET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mClover
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86930-plasmid-map-sequence-id-174752

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  17. pET MBP-FlAsH LIC cloning vector (MBP-FlAsH)

    Plasmid
    #30185
    Depositor
    Scott Gradia
    Type
    Empty backbone
    Use
    Tags
    6xHis, AviTag (biotin), FlAsH, MBP, and TEV cleav…
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    Aug. 17, 2011
    Availability
    Academic Institutions and Nonprofits only
    30185-plasmid-map-sequence-id-13873

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  19. pET MBP tdTomato LIC cloning vector (MBP-tdTomato)

    Plasmid
    #86927
    Purpose
    pET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-tdTomato
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86927-plasmid-map-sequence-id-181702

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Showing: 381 - 400 of 12605 results