Ready-to-use AAV Retrograde particles produced from pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (#125712). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE plasmid DNA. CaMKIIa-driven expression of the optimized mosquito OPN3 rhodopsin in-frame with mScarlet. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

Stable expression of ANXA11-mRuby3 (Annexin A11-mRuby3) in mammalian cells

Plasmid encoding E2-Crimson

A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).

In vivo recombination assay fluorescent reporter

Plasmid encoding mCherry-L

Expression of gene in mammalian cells by retroviral transduction

arabinose-induced synthesis of a hybrid RNA containing a 5' MS2 hairpin, E. coli chiP 5'UTR insert between XmaI and HindIII sites, surrounded by a 7bp GC clamp, and followed by a 3' trpA terminator.

Expression of gene in mammalian cells by retroviral transduction

Expression of gene in mammalian cells by retroviral transduction

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

PLlacO-1:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrA