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pAAV2-GCaMP5g-P2A-Vmn1r58

Plasmid

#133418

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 58

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP5g-P2A-PAmCherry-Vmn1r58 (Vmn1r58 Mouse)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-FLEX-jYCaMP1

Plasmid

#135422

Purpose

Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection

Depositor

Kaspar Podgorski

Article

Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

Insert

jYCaMP1

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pKL12-GCaMP6f-pHuji bb118

Plasmid

#158981

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to pHuji fluorophores in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f-pHuji

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833 a…

Promoter

Bba_J23118 (sigma 70)

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCW57-Cx32-IRES-GCaMP6s

Plasmid

#216795

Purpose

Inducible bicistronic lentiviral plasmid for the simultaneous expression of Connexin 32 and cytosolic GCaMP6s

Depositor

Fabio Mammano

Article

Tettey-Matey et al Cell Commun Signal. 2024 Dec 5;22(1):589

Insert

Connexin 32-IRES-GCaMP6s (GJB1 Human)

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAS1 - pRig-3::GCaMP6f::unc-54

Plasmid

#194970

Purpose

Fluorescent reporter of cytosolic calcium in C.elegans.

Depositor

Frederic Hoerndli

Insert

GCaMP6f

Expression

Worm

Promoter

rig-3

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pKK1 -pFlp-18::mito4x-GCaMP6f

Plasmid

#194969

Purpose

Fluorescent reporter of mitochondrial matrix Ca2+.

Depositor

Frederic Hoerndli

Insert

4 repeats of Cox8 presequence plus GCaMP6f

Expression

Worm

Promoter

Flp-18

Available Since

June 21, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pOttc1495 - pAAV CaMKII SERCaMP_ASARTDL

Plasmid

#192602

Purpose

An AAV packaging vector that expresses SERCaMP under control of the CaMKII promoter.

Depositor

Brandon Harvey

Insert

SERCaMP

Use

AAV

Promoter

CaMKII

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pUASTattb-cAMPr Y205A, R626A

Plasmid

#84932

Purpose

cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.

Depositor

Justin Blau

Article

Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

Insert

cAMPr Y205A R626A

Expression

Insect

Mutation

PKA-C Y205A, PKA-R R626A

Promoter

5X UAS

Available Since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-synp-F-H2B-GCaMP6f

Plasmid

#102994

Purpose

Expresses histone fused GCaMP6f under the synapsin promoter

Depositor

David Cox

Article

Skocek et al Nat Methods. 2018 Jun;15(6):429-432. doi

Insert

H2B-GCaMP6f

Use

AAV

Expression

Mammalian

Promoter

human synapsin I promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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PRS_8-jGCaMP7b-P2A-mRuby3

Plasmid

#192595

Purpose

AAV-vector for red fluorescent labeling and green fluorescent calcium measurements from noradrenergic neurons

Depositor

Simon Wiegert

Insert

jGCaMP7b

Use

AAV

Tags

mRuby3

Expression

Mammalian

Promoter

PRSX8

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET-triple mutation-pa-GCaMP6f

Plasmid

#67570

Purpose

bacterial expression of a photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

pa-GCaMP6f

Tags

HIS-tag

Expression

Bacterial

Mutation

V115H, L64F, T65S

Promoter

T7

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only
pSF-aTubCaud-FLAG-GCaMP8s

Plasmid

#251708

Purpose

Paramecium caudatum codon-optimized GCaMP8s fluorescent reporter for calcium signaling

Depositor

Daniel Fürth

Insert

GCaMP8s

Use

Protozoan expression

Tags

FLAG

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-DIO-LifeCamp

Plasmid

#251726

Purpose

Expression of Cre-dependent calcium lifetime sensor LifeCamp under hSyn promoter in pAAV backbone

Depositor

Bernardo Sabatini

Article

Lodder et al bioRxiv 2025.12.23.696288

Insert

LifeCamp

Use

AAV

Expression

Mammalian

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-DIO-LifeCamp0

Plasmid

#251727

Purpose

Expression of Cre-dependent binding mutant control calcium lifetime sensor LifeCamp0 under hSyn promoter in pAAV backbone

Depositor

Bernardo Sabatini

Article

Lodder et al bioRxiv 2025.12.23.696288

Insert

LifeCamp0

Use

AAV

Expression

Mammalian

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-RiboL1-LifeCamp

Plasmid

#251728

Purpose

Expression of soma-targeted calcium lifetime sensor LifeCamp under hSyn promoter in pAAV backbone

Depositor

Bernardo Sabatini

Article

Lodder et al bioRxiv 2025.12.23.696288

Insert

RiboL1-LifeCamp

Use

AAV

Expression

Mammalian

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn:FLEX-Soma-jGCaMP8m

Plasmid

#239670

Purpose

Cre-dependent expression of Soma-jGCaMP8m under hSyn promoter.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Soma-jGCaMP8m

Use

AAV

Tags

6xHis and EE-RR

Expression

Mammalian

Promoter

hSyn

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn:FLEX-Soma-jGCaMP8s

Plasmid

#239669

Purpose

Cre-dependent expression of Soma-jGCaMP8s under hSyn promoter.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Soma-jGCaMP8s

Use

AAV

Tags

6xHis and EE-RR

Expression

Mammalian

Promoter

hSyn

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn:FLEX-Soma-jGCaMP8f

Plasmid

#239671

Purpose

Cre-dependent expression of Soma-jGCaMP8f under hSyn promoter.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Soma-jGCaMP8f

Use

AAV

Tags

6xHis and EE-RR

Expression

Mammalian

Promoter

hSyn

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-SPARC2-S-13xLexAop-GCaMP8m_VK05

Plasmid

#238557

Purpose

A LexAop-based SPARC plasmid for SPARC-L. Can also be used as a standalone sparsener of a LexA driver line. Sparse (S) density. The GCaMP ORF can be swapped for your gene of interest.

Depositor

Tom Clandinin

Article

Currier et al Cell. 2025 Aug 7;188(16):4366-4381.e14.

Insert

GCaMP8m

Use

Sparc plasmid

Tags

DsRed-Express

Expression

Insect

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-SPARC2-I-13xLexAop-GCaMP8m_VK05

Plasmid

#238556

Purpose

A LexAop-based SPARC plasmid for SPARC-L. Can also be used as a standalone sparsener of a LexA driver line. Intermediate (I) density. The GCaMP ORF can be swapped for your gene of interest.

Depositor

Tom Clandinin

Article

Currier et al Cell. 2025 Aug 7;188(16):4366-4381.e14.

Insert

GCaMP8m

Use

Sparc plasmid

Tags

DsRed-Express

Expression

Insect

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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