We narrowed to 1,118 results for: gc

1. pUC19 gene 60 thru gap + Cys

   Plasmid

   #49161

   Purpose

   Template for generating gene 60 mRNA truncated two codons after coding gap + Cys

   Depositor

   Nils Walter

   Article

   Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

   Insert

   Bacteriophage T4 gene 60
   

   Use

   Tags

   Expression

   Mutation

   gene 60 mRNA truncated two codons after coding ga…

   Promoter

   T7

   Available since

   Feb. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Lyn-FRB-DMVF-181-N

   Plasmid

   #87609

   Purpose

   FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to plasma membrane.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   Lyn-FRB-DMVF181-N
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-FKBP-DMVF-181-C

   Plasmid

   #87611

   Purpose

   FKBP-tagged C-terminal Dronpa fragment for BiFC.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   FKBP-DMVF181-C
   

   Use

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGLU_86

   Plasmid

   #225801

   Purpose

   Non-functional, randomly-generated sequence of 165 with 50% GC

   Depositor

   Nili Ostrov

   Insert

   Dummy_[Himar]
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CW3SL-EGFP

   Plasmid

   #61463

   Purpose

   AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPHT-PKA

   Plasmid

   #60936

   Purpose

   Fluorescent FPX sensor for cAMP

   Depositor

   Anne Marie Quinn

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   PKA FPX Sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. gRNA-trp-HYB

   Plasmid

   #64331

   Purpose

   trp1 deletion gRNA cassette carried by pRS42H

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   gBlock product of trp1 deletion gRNA cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

8. gRNA-ura-HYB

   Plasmid

   #64330

   Purpose

   ura3 deletion gRNA cassette carried by pRS42H

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   gBlock product of ura3 deletion gRNA cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

9. gRNA-leu-HYB

   Plasmid

   #64332

   Purpose

   leu2 deletion gRNA cassette carried by pRS42H

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   gBlock product of leu2 deletion gRNA cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

10. gRNA-his-HYB

    Plasmid

    #64333

    Purpose

    his3 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of his3 deletion gRNA cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pGM32DEST

    Plasmid

    #74747

    Purpose

    Encodes for the QF-GR recombinant gene, splice linker, and mCherry reporter gene

    Depositor

    Jordan Ward, Keith Yamamoto

    Article

    Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

    Inserts

    Gateway cassette
    QF-GR (NR3C1 Synthetic, Neurospora crassa, Human)
    

    Use

    Tags

    Fusion of the Neurospora crassa QF activator to t…

    Expression

    Worm

    Mutation

    Promoter

    No promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. -227 hTF

    Plasmid

    #15442

    Depositor

    Nigel Mackman

    Article

    Oeth et al Arterioscler Thromb Vasc Biol. 1997 Feb

    Insert

    Tissue factor promoter (F3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2007

    Availability

    Academic Institutions and Nonprofits only

13. HsCLYBL-FLAG

    Plasmid

    #111290

    Purpose

    mammalian expression

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    human CLYBL (CLYBL Human)
    

    Use

    Tags

    linker and FLAG tag: TGGGSGGSGGSMDYKDDDDK

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMBPCas2/3

    Plasmid

    #89238

    Purpose

    Plasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusion

    Depositor

    Blake Wiedenheft

    Article

    Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

    Insert

    Cas2/3
    

    Use

    Tags

    Strep II-MBP

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-baPrs-hdNadV

    Plasmid

    #91950

    Purpose

    pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi

    Depositor

    George Cătălin Marinescu

    Article

    Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

    Inserts

    Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
    Phosphoribosyl Pyrophosphate Synthetases
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed Leucine 135 to Isoleucine

    Promoter

    T7

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-LifeAct-FRB-Venus-173-N

    Plasmid

    #87616

    Purpose

    FRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    LifeAct-FRB-Venus173-N
    

    Use

    Tags

    17-aa sequence from Abp140 (aka "Lifeact&quo…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAWp9-5

    Plasmid

    #73856

    Purpose

    pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1

    Depositor

    Timothy Lu

    Article

    Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

    Insert

    UBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pAWp9-4

    Plasmid

    #73855

    Purpose

    pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg1-U6p-GFP-sg1

    Depositor

    Timothy Lu

    Article

    Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

    Insert

    UBCp-RFP-CMVp-GFP-U6p-RFP-sg1-U6p-GFP-sg1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-LifeAct-FRB-DMVF-181-N

    Plasmid

    #87610

    Purpose

    FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to actin cytoskeleton.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    LifeAct-FRB-DMVF181-N
    

    Use

    Tags

    17-aa sequence from Abp140 (aka "Lifeact&quo…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti CMV MCL1 Puro

    Plasmid

    #140746

    Purpose

    Expresses MCL1 in mammalian cells

    Depositor

    Scott Dixon

    Article

    Inde et al Cell Rep. 2020 Jul 7;32(1):107845. doi:

    Insert

    MCL1 (MCL1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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