We narrowed to 705 results for: gc

1. pTBL322 GC

   Plasmid

   #126950

   Purpose

   To target a protospacer with GC at the cut site.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human genome with GC at cut site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOpen-NotIR (GC)

   Plasmid

   #165507

   Purpose

   Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.

   Depositor

   Drew Endy, Jennifer Molloy, FreeGenes Project

   Insert

   NotI (GC-rich recognition site)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pTBL401 GC sgRNA

   Plasmid

   #126435

   Purpose

   To target a protospacer with GC at the cut site.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human genome with GC at cut site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLENTI6.3/AR-GC-E2325

   Plasmid

   #85128

   Purpose

   exogenous AR expression

   Depositor

   Karl-Henning Kalland

   Article

   Olsen et al BMC Cancer. 2016 Jul 4;16:377. doi: 10.1

   Insert

   androgen receptor (AR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCR3-FLAG-Gephyrin GC

   Plasmid

   #68818

   Purpose

   Flag tagged mammalian expression of GC domain

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   NIL

   Promoter

   CMV

   Available since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. hp-66%GC-40bp

   Plasmid

   #169200

   Purpose

   Force-activating hairpin for 3'-to-5' helicase

   Depositor

   Tom Perkins

   Article

   Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

   Insert

   66%GC-40bp-hairpin
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hp-75%GC-20bp

   Plasmid

   #169201

   Purpose

   Force-activating hairpin for 3'-to-5' helicase

   Depositor

   Tom Perkins

   Article

   Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

   Insert

   75%GC-20bp-hairpin
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. hp-75%GC-40bp

   Plasmid

   #169202

   Purpose

   Force-activating hairpin for 3'-to-5' helicase

   Depositor

   Tom Perkins

   Article

   Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

   Insert

   75%GC-40bp-hairpin
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. hp-100%GC-20bp

   Plasmid

   #169203

   Purpose

   Force-activating hairpin for 3'-to-5' helicase

   Depositor

   Tom Perkins

   Article

   Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

   Insert

   100%GC-20bp-hairpin
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hp-50%GC-20bp

    Plasmid

    #169196

    Purpose

    Force-activating hairpin for 3'-to-5' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    50%GC-20bp-hairpin
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. hp-50%GC-40bp

    Plasmid

    #169197

    Purpose

    Force-activating hairpin for 3'-to-5' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    50%GC-40bp-hairpin
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. hp-66%GC-20bp

    Plasmid

    #169199

    Purpose

    Force-activating hairpin for 3'-to-5' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    66%GC-20bp-hairpin
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p3E-APEX2 (GC rich)

    Plasmid

    #115550

    Purpose

    Multisite gateway vector for 3' tagging with GC-rich APEX2. Used to make 115563. Parton lab clone HHS

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    APEX2 (GC rich)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. eGFP Y93H GC editing reporter

    Plasmid

    #198884

    Purpose

    Express eGFP fluorescence after Y93H correction and mCherry as transfection control

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Y93H, Gly92(GGT-to-GGG)

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. hp-50%GC-40bp-Revbindsite

    Plasmid

    #169198

    Purpose

    Force-activating hairpin for 5'-to-3' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    50%GC-40bp-hairpin-binding-site-reversed
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRK5-HA-GST-4EBP1-Low GC

    Plasmid

    #69018

    Purpose

    expresses tagged 4EBP1

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    EIF4EBP1 (EIF4EBP1 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Mammalian

    Mutation

    lowGC content, wt is ~70% GC rich. Still, it code…

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. LbCas12a-DR-GC-swap-cr in pBCSK+

    Plasmid

    #126643

    Purpose

    Cloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    LbCas12a Full Length Direct Repeat GC Swap crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. FnoCas12a-DR-GC-swap-cr in pBCSK+

    Plasmid

    #126644

    Purpose

    Cloning vector FnoCas12a-DR-GC-swap-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    FnoCas12a Full Length Direct Repeat GC Swap crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hp-50%GC-Left-half-120bp

    Plasmid

    #169204

    Purpose

    Force-activating hairpin for 3'-to-5' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    50%GC-Left-half-120bp-hairpin
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hp-50%GC-Right-half-120bp

    Plasmid

    #169205

    Purpose

    Force-activating hairpin for 3'-to-5' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    50%GC-Right-half-120bp-hairpin
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge