We narrowed to 1,107 results for: gibson assembly
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Plasmid#246868PurposeExpress HaloTag-P2A-RSV N-1xSunTag@105AA (Nexo-SunTag) for RSV imaging.DepositorInsertHaloTag-P2A-RSV N-1xSunTag@105AA
UseLentiviralTagsHaloTagExpressionMammalianMutationThe RSV nucleoprotein (N) with an insertion of a …PromoterSFFVAvailable SinceJan. 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
pMMACE DEST CMV H2B iRFP
Plasmid#206253PurposeDEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes H2B iRFP713 under the control of CMV promoter. CRE-Acceptor in MultiBac system.DepositorInsertH2B iRFP713
UseRecombinant baculovirus production, multimate/gat…TagsiRFP713ExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 4 CMV GST mTagBFP
Plasmid#206259PurposeENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.DepositorInsertGTS mTagBFP
UseMultimate/gateway entr 4TagsBFGT1 (Golgi localisation peptide)ExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMDS DEST
Plasmid#206264PurposeDEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. After LR propagate in Pir+. Works as a CRE-donor in the MultiBac system.DepositorTypeEmpty backboneUseCre-donor, multimate/gateway dest vector (multiba…Available SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
MZB134
Plasmid#228485Purpose"Empty" N-terminal 6xHis-TEV-Halotag-HRV vector for easy DNA Assembly for pulldowns (Bacterial Expression)DepositorTypeEmpty backboneTags6xHis-TEV-Halo-HRVExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCA_e-2
Plasmid#202097PurposeReceiver cloning vector for even-2 level constructs. Contains lacZ for blue/white screening.DepositorTypeEmpty backboneUseSynthetic BiologyMutationNoneAvailable SinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCA_e-1
Plasmid#202096PurposeReceiver cloning vector for even-1 level constructs. Contains lacZ for blue/white screening.DepositorTypeEmpty backboneUseSynthetic BiologyMutationNoneAvailable SinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0R_lacZ
Plasmid#202095PurposeReceiver cloning vector for making level 0 parts. Contains lacZ for blue/white screening.DepositorTypeEmpty backboneUseSynthetic BiologyMutationNoneAvailable SinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCA_o-4
Plasmid#202094PurposeReceiver cloning vector for odd-4 level constructs. Contains lacZ for blue/white screening.DepositorTypeEmpty backboneUseSynthetic BiologyMutationNoneAvailable SinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCA_o-3
Plasmid#202093PurposeReceiver cloning vector for odd-3 level constructs. Contains lacZ for blue/white screening.DepositorTypeEmpty backboneUseSynthetic BiologyMutationNoneAvailable SinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCA_o-2
Plasmid#202092PurposeReceiver cloning vector for odd-2 level constructs. Contains lacZ for blue/white screening.DepositorTypeEmpty backboneUseSynthetic BiologyMutationNoneAvailable SinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCA_o-1
Plasmid#202091PurposeReceiver cloning vector for odd-1 level constructs. Contains lacZ for blue/white screening.DepositorTypeEmpty backboneUseSynthetic BiologyMutationNoneAvailable SinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP
Plasmid#185687PurposeLow-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).DepositorInsertERmembrane-GST-2xHaloTag-KKMP
ExpressionMammalianAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJK2067
Plasmid#171845PurposeC. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)DepositorInserthis-58, eGFP, mCherry
TagsN-terminal Tag OLLAS, V5 and C-terminal Tag PEST…ExpressionWormMutationboxB wild type and hairpin mutantPromotermex-5 promoterAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-GEI-17(133-509)
Plasmid#87056PurposeExpresses 6xHis-tagged gei-17 (isoform f), with a TEV protease cleavage site between the tag and the ORFDepositorInsertGEI-17 isfoomr f (aa 133-509) (gei-17 Nematode)
Tags6xHis-TEV and GAGGGS linkerExpressionBacterialPromoterT7Available SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH509
Plasmid#102664PurposeTetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
C76m
Plasmid#120988PurposeMoClo golden gate assembly CD part for KanR (kanamycin resistance protein). Please see Supplemental Documents for annotated Genbank file.DepositorInsertKanR
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C9m
Plasmid#120958PurposeMoClo golden gate assembly CD part for deCFP (cyan fluorescent protein) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertdeCFP
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pL0_EF-PtBle
Plasmid#202180PurposeLevel 0 plasmid containing a phleomycin antibiotic cassette (fcpF promoter) with EF overhangs used to build a level 1 construct.DepositorInsertShBle expression cassette
UseSynthetic BiologyMutationNoneAvailable SinceAug. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_CD-greenlantern
Plasmid#202141PurposeLevel 0 plasmid containing a green lantern fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInsertGreen lantern gene
UseSynthetic BiologyMutationNoneAvailable SinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits