We narrowed to 6,705 results for: hwa

1. pARC8-LambdaRedBeta-EcSSB-EcMutLE32K

   Plasmid

   #162576

   Purpose

   Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein.

   Depositor

   George Church

   Article

   Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038

   Inserts

   Lambda Red-Beta
   E. coli SSB
   EcMutLE32K
   

   Expression

   Bacterial

   Mutation

   E32K mutated to give dominant negative phenotype

   Promoter

   pBAD

   Available Since

   May 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-EF1a-FLEx-ASAP4b-Kv-WPRE

   Plasmid

   #201032

   Purpose

   Expresses somatically enriched ASAP4b from the EF-1alpha promoter in a cre-dependent manner

   Depositor

   Michael Lin

   Article

   Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

   Insert

   ASAP4e-Kv
   

   Use

   AAV

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. YFP-FKBP (YF)

   Plasmid

   #20175

   Depositor

   Tobias Meyer

   Article

   Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

   Insert

   YFP-FKBP (YF): (FKBP1A Human)
   

   Expression

   Mammalian

   Mutation

   FKBP was taken out from pC4M-F2E with a flanking …

   Available Since

   March 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-U6-CNCB_sgEIF3D-4

   Plasmid

   #236766

   Purpose

   A piggybac-based vector containing mouse U6 promoter-driven EIF3D sgRNA #4 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

   Depositor

   Kazutoshi Takahashi

   Article

   Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

   Insert

   EIF3D sgRNA-4 (EIF3D Human)
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   mouse U6 promoter and mouse U6 promoter, CAG prom…

   Available Since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1-shCELF4-4405

   Plasmid

   #115466

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   shCELF4-4405 (CELF4 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-hSyn-ASAP4b-Kv-WPRE

   Plasmid

   #201030

   Purpose

   Expresses somatically enriched ASAP4b in neurons, can be used to package AAV

   Depositor

   Michael Lin

   Article

   Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

   Insert

   ASAP4b-Kv
   

   Use

   AAV

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1REST_sh_seq2_WPRE

   Plasmid

   #127574

   Purpose

   shRNA to knock down RE1-silencing transcription factor (REST)

   Depositor

   Malin Parmar

   Article

   Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

   Insert

   shRNA for knock down of REST (REST Human)
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX317-CELF4

   Plasmid

   #115445

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   CELF4 (CELF4 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. mVenus-proα1(I)

   Plasmid

   #119845

   Purpose

   Expresses mouse Type I procollagen α1 chain (Col1a1) with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Article

   Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

   Insert

   Type I procollagen α1 chain (Col1a1 Mouse)
   

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Col1a1 exons 2-3 replaced by fluorescent tag

   Promoter

   CMV

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRetroX SgK269 WT

    Plasmid

    #68028

    Purpose

    expression of Sgk269 in mammalian cells

    Depositor

    Roger Daly

    Article

    Croucher et al Cancer Res. 2013 Mar 15;73(6):1969-80. d

    Insert

    PEAK1 (PEAK1 Human)
    

    Use

    Retroviral

    Tags

    FLAG/myc, HA, and IRES-DsRed

    Expression

    Mammalian

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pLKO.1-shCELF4-4404

    Plasmid

    #115465

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shCELF4-4404 (CELF4 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK5 FLAG 17xS/T->A, catalytic active lipin 1

    Plasmid

    #32007

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 (Lpin1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S106A, S150A, (S281A, T282A), S285A, S287A, S293A…

    Promoter

    CMV, SP6

    Available Since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

13. CPSF4

    Plasmid

    #155477

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CPSF4 (CPSF4 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNK7187

    Plasmid

    #219761

    Purpose

    MoClo-compatible Level 1 for expression PpASCL in plants (N.benthamiana, BY2 cells)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    PpASCL
    

    Expression

    Plant

    Promoter

    p35s_0.4kb - 5'UTR TMV omega

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. CPSF4-1

    Plasmid

    #155478

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CPSF4 (CPSF4 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAF92

    Plasmid

    #135929

    Purpose

    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil mCherry-TNFSF9 (type II transmembrane) fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFSF9).

    Depositor

    Andrew S. Flies

    Article

    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

    Insert

    mCherry fused to TNFSF9
    

    Use

    Transposon

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQCXIP mScarlet-MOSPD2 RD/LD

    Plasmid

    #186476

    Purpose

    Expression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to mScarlet in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Retroviral

    Tags

    mScarlet

    Expression

    Mammalian

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. THOC4

    Plasmid

    #156083

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    THOC4 (ALYREF Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX303-PCDH7-d

    Plasmid

    #108541

    Purpose

    Expresses human PCDH7 transcript variant d in mammalian cells

    Depositor

    Kathryn O’Donnell

    Article

    Zhou et al Cancer Res. 2017 Jan 1;77(1):187-197. do

    Insert

    PCDH7 transcript variant d (PCDH7 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti_EF1α-NES-Kinprola_PKA-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid

    #233371

    Purpose

    EF1α driven co-expression of the PKA activity recorder Kinprola_PKA fused to mEGFP and Cas9 expression through lentivirus transduction

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Inserts

    NES-Kinprola_PKA-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    

    Use

    Lentiviral

    Tags

    FLAG, NES, and mEGFP

    Promoter

    EF1α

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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