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pcDNA4/HisMaxB-YAP1-S127A

Plasmid

#18988

Depositor

Marius Sudol

Publication

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Yes-kinase associated protein S127A mutant (YAP1 Human)

Tags

His

Expression

Mammalian

Mutation

Serine 127 changed to Alanine, mutated protein t…

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
p3XFLAG-CMV-14-WDR5

Plasmid

#59974

Purpose

Mammalian Expression of WDR5-FLAG

Depositor

Debu Chakravarti

Publication

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

WDR5 (WDR5 Human)

Tags

FLAG

Expression

Mammalian

Available since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pHAGE-PDGFRA

Plasmid

#116769

Purpose

Lentiviral expression of PDGFRA

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PDGFRA (PDGFRA Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA4/HisMaxB-YAP1-S127A

Plasmid

#18988

Depositor

Marius Sudol

Publication

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Yes-kinase associated protein S127A mutant (YAP1 Human)

Tags

His

Expression

Mammalian

Mutation

Serine 127 changed to Alanine, mutated protein t…

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
p3XFLAG-CMV-14-WDR5

Plasmid

#59974

Purpose

Mammalian Expression of WDR5-FLAG

Depositor

Debu Chakravarti

Publication

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

WDR5 (WDR5 Human)

Tags

FLAG

Expression

Mammalian

Available since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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mKate2-P2A-APEX2-2xFYVE_hrs

Plasmid

#67663

Purpose

Mammalian expression vector driving APEX2-2xFYVE (2xMouse FYVE domain from HRS, [marks PI3P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mKate2-P2A-APEX2-2xFYVE

Expression

Mammalian

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-PDGFRA

Plasmid

#116769

Purpose

Lentiviral expression of PDGFRA

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PDGFRA (PDGFRA Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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AAVS1-idCas9-vpr

Plasmid

#89985

Purpose

inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus

Depositor

Jie Na

Publication

Guo et al Protein Cell. 2017 Jan 23. doi: 10.1007/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-VPR

Expression

Mammalian

Promoter

TRE-tight

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDNA2.0 6H-TEV-KRAS

Plasmid

#111849

Purpose

Expresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

Depositor

Kenneth Westover

Publication

Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

Insert

KRAS (KRAS Human)

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Promoter

T5

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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GFP-TRIM33

Plasmid

#65398

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Publication

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRIM33 (TRIM33 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pWZL Neo Myr Flag PDK1

Plasmid

#20564

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PDK1 (PDK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET StrepII-StrepII TEV LIC cloning vector (2RRT)

Plasmid

#55215

Depositor

Scott Gradia

Type

Empty backbone

Tags

2xStrep II and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAVS1-idCas9-vpr

Plasmid

#89985

Purpose

inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus

Depositor

Jie Na

Publication

Guo et al Protein Cell. 2017 Jan 23. doi: 10.1007/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-VPR

Expression

Mammalian

Promoter

TRE-tight

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pDNA2.0 6H-TEV-KRAS

Plasmid

#111849

Purpose

Expresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

Depositor

Kenneth Westover

Publication

Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

Insert

KRAS (KRAS Human)

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Promoter

T5

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
GFP-TRIM33

Plasmid

#65398

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Publication

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRIM33 (TRIM33 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pWZL Neo Myr Flag PDK1

Plasmid

#20564

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PDK1 (PDK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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plenti6-SLC7A11/xCT-V5

Plasmid

#170427

Purpose

Eepresses SLC7A11 (also named xCT) in mammalian cells

Depositor

Boyi Gan

Publication

Koppula et al J Biol Chem. 2017 Aug 25;292(34):14240-1

Insert

solute carrier family 7 member 11 (SLC7A11 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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FLAG-NLRC5

Plasmid

#37521

Depositor

Thomas Kufer

Publication

Neerincx et al J Biol Chem. 2010 Aug 20;285(34):26223-3

Insert

NLRC5 (NLRC5 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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MSCV-FOXP3-IRES-Thy1.1

Plasmid

#17443

Depositor

Anjana Rao

Publication

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDONR223 SARS-CoV-2 ORF7B

Plasmid

#141277

Purpose

Gateway-compatible Entry vector, with insert of ORF7B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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