We narrowed to 9,800 results for: otos

1. pCIneoMyc UNC119Ab

   Plasmid

   #128332

   Purpose

   Mammalian expression vector of Myc-tagged human UNC119A isoform b

   Depositor

   Yutaka Hata

   Article

   Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

   Insert

   UNC119 (UNC119 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRXIP-GFP-OSBP(PHD)

   Plasmid

   #221745

   Purpose

   Stable expression of GFP-OSBP(PHD) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   OSBP (OSBP Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   amino acids 87–185

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Sec61β-Flag-APEX2-SOPP3

   Plasmid

   #229072

   Purpose

   Expresse APEX2-SOPP3 in ER in mammalian cell

   Depositor

   Weijun Pan

   Article

   Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

   Insert

   Express APEX2-SOPP3 in the ER (APEX2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCOTS-pyl-GFP(35TAG)

   Plasmid

   #92047

   Purpose

   This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.

   Depositor

   Lital Alfonta

   Article

   Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

   Inserts

   EGFP
   Pyrrolysyl tRNA(cua)
   Pyrrolysyl tRNA synthetase (methanosarcina mazei)
   

   Use

   Replicative expression plasmid for cyanobacteria …

   Tags

   Histag

   Expression

   Mutation

   Tyrosine in position 35 of the GFP was mutated f…

   Promoter

   LeuP (native S. elongatus promoter), PpsbII, and …

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pECE-M2-CDC27 wt

   Plasmid

   #31661

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   cell division cycle 27 homolog (CDC27 Human)
   

   Use

   Tags

   M2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pCAGGS-hPhyB-mCherry-HRasCT

   Plasmid

   #100281

   Purpose

   Expresses Phytochrome B (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   Phytochrome B (PHYB Mustard Weed)
   

   Use

   Tags

   mCherry fused with HRas C-terminus for plasma mem…

   Expression

   Mammalian

   Mutation

   Human codon optimized PhyB (1-908 aa)

   Promoter

   CAG promoter

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. LT3GEPIR-ARshRNA1

   Plasmid

   #220561

   Purpose

   To inducibly knockdown AR expression

   Depositor

   Sean Lee

   Article

   Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

   Insert

   AR shRNA1 (AR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE3G (TetOP) promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-rBrokenheart

   Plasmid

   #203394

   Purpose

   AAV transfer plasmid containing a constitutive (non Cre-dependent) BrokenHeart construct: a piggyBac donor transposon interrupting tdTomato. Transposase activity rescues coding sequence.

   Depositor

   Joseph Dougherty, Rob Mitra

   Article

   Yen et al Curr Protoc. 2023 Sep;3(9):e883. doi: 10

   Insert

   Brokenheart
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. MOM603

   Plasmid

   #133242

   Purpose

   expresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cells

   Depositor

   Shinsuke Niwa

   Article

   Chiba et al Proc Natl Acad Sci U S A. 2019 Aug 27. p

   Insert

   kif1a (KIF1A Human)
   

   Use

   Tags

   StrepII tag and mScarlet

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hsyn-flex-Voltron

    Plasmid

    #119035

    Purpose

    Cre-dependant expression of Voltron in neurons

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Insert

    Voltron
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hsyn

    Available since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-HA-eEF2K(S441A/S445A)

    Plasmid

    #110161

    Purpose

    Expression of human eEF2K (S441A/S445A) in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    Kruiswijk et al Sci Signal. 2012 Jun 5;5(227):ra40. doi:

    Insert

    eEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S441A/S445A

    Promoter

    CMV

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX VAMP7 (1-188)

    Plasmid

    #42315

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 Cytoplasmic domain (1-181) (VAMP7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Δ181-220

    Promoter

    Ptac

    Available since

    March 12, 2013

    Availability

    Academic Institutions and Nonprofits only

13. PEX3*-SBP-GFP

    Plasmid

    #120174

    Purpose

    Expresses a chimera of the peroxisomal-targeting signal of PEX3, SBP tag and GFP (fluorescent tag)

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    Peroxisomal biogenesis factor 3 (PEX3 Human)
    

    Use

    Tags

    SBP and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-Zdk1-EB1C

    Plasmid

    #107696

    Purpose

    C-terminal half of π-EB1, EGFP-tagged, dissociates from microtubule ends in blue light

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE1 (MAPRE1 Human)
    

    Use

    Tags

    EGFP and Zdk1

    Expression

    Mammalian

    Mutation

    EB1 aa 186-268

    Promoter

    CMV

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX6P1-human full-length LIC1

    Plasmid

    #74597

    Purpose

    used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    full length human dynein light intermediate chain 1 (DYNC1LI1 Human)
    

    Use

    Tags

    N-terminal GST and Strep II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHX_HDR_hPAX7_IRES-EGFP-PGK-Neo

    Plasmid

    #160458

    Purpose

    Homology-directed recombination donor vector for EGFP reporter knock-in to human PAX7 3' UTR

    Depositor

    April Pyle

    Article

    Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

    Inserts

    PAX7 Left/5' Homology Arm (PAX7 Human)
    PAX7 Right/3' Homology Arm (PAX7 Human)
    

    Use

    Insertion of a fluorescent reporter through homol…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-mCherry-p2A-full length LIC1

    Plasmid

    #74601

    Purpose

    expresses LIC1 in mammalian cells; untagged but cells indicate expression with diffuse mCherry

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    full length human dynein light intermediate chain 1 (DYNC1LI1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-syn-jYCaMP1

    Plasmid

    #135423

    Purpose

    Yellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfection

    Depositor

    Kaspar Podgorski

    Article

    Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

    Insert

    jYCaMP1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBK086

    Plasmid

    #183145

    Purpose

    pET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expression

    Depositor

    Daan Swarts

    Article

    Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

    Insert

    MapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP VAMP7 (1-120)

    Plasmid

    #42317

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 Longin domain (1-120) (VAMP7 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Δ120-220

    Promoter

    CMV

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

21. N-Flag-IPO9_Lenti SFFV Puro WPRE

    Plasmid

    #217916

    Purpose

    Express IPO9 in mammalian cells

    Depositor

    Benjamin Cravatt

    Article

    Ogasawara et al Cell Chem Biol. 2024 Nov 7:S2451-9456(24

    Insert

    importin 9 (IPO9 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-CaMKII-Chronos-GFP

    Plasmid

    #58805

    Purpose

    AAV expression of Chronos-GFP under the CaMKII promoter

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. pFrt-invCAG-ires-Luc

    Plasmid

    #63576

    Purpose

    Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional cDNA and Luc expression upon integration. The vector contains an FRT site and two mut loxP sites.

    Depositor

    Ivo Huijbers

    Article

    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi

    Insert

    Stop-Lox71-IRES-Luciferase-pA-FRT-Lox66-reverseCAG
    

    Use

    Cre/Lox; Frt/flpe recombination mediated insertion

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. FLAG-MARK2-CR

    Plasmid

    #229421

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    MARK2-CR (MARK2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    silent mutation CRISPR resistant

    Promoter

    EFS

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLEX307-SARS-CoV-2-3CL-C145A

    Plasmid

    #160279

    Purpose

    Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease with C145A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    C145A

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP-MYO10-HMM

    Plasmid

    #87256

    Purpose

    Expression plasmid to perform NanoSPD assays in mammalian cells (generates EGFP-tagged bait).

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYO10 (Myo10 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Myo10 is truncated to contain aa.1-941

    Promoter

    CMV

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pmCherry-Zdk1-EB1C

    Plasmid

    #107695

    Purpose

    C-terminal half of π-EB1, mCherry-tagged, dissociates from microtubule ends in blue light

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE1 (MAPRE1 Human)
    

    Use

    Tags

    Zdk1 and mCherry

    Expression

    Mammalian

    Mutation

    EB1 aa 186-268

    Promoter

    CMV

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1(+)-mCherry-p115

    Plasmid

    #181996

    Purpose

    Mammalian expression vector encoding p115 RhoGEF tagged with mCherry.

    Depositor

    Jin Zhang

    Article

    Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

    Insert

    mCherry-p115 (ARHGEF1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEGFP-MYO7A-TAIL

    Plasmid

    #89585

    Purpose

    Expression plasmid for a positive control bait protein in NanoSPD 2.0 assays.

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYO7A (Myo7a Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Truncation encoding amino acids 870-2166 of MYO7A…

    Promoter

    CMV

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. hRAD18 dC2-EGFP

    Plasmid

    #68826

    Purpose

    Expresses human RAD18 (deleting Polymerase eta binding domain) tagged with EGFP

    Depositor

    Satoshi Tateishi

    Article

    Tsuji et al Genes Cells. 2008 Apr;13(4):343-54. doi:

    Insert

    human RAD18 (deleting Polymerase eta binding domain) tagged with EGFP (RAD18 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only