We narrowed to 26,608 results for: Mut

1. pT7-7 asyn E46K

   Plasmid

   #105728

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

   Insert

   alpha-synuclein E46K (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E46K

   Promoter

   T7

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFR D770-N771 insNPG

   Plasmid

   #11016

   Purpose

   Retroviral construct for expressing human EGFR with D770-N771 insNPG mutation in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR D770-N771 insNPG (EGFR Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   D770-N771 insNPG

   Promoter

   Available Since

   Dec. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

3. EGFR (del3) L747-E749del, A750P

   Plasmid

   #11015

   Purpose

   Retroviral construct for expressing human EGFR with L747-E749 deletion and A750P mutation in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR (del3) (EGFR Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   L747-E749 deleted, A750P

   Promoter

   Available Since

   Dec. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA6 asyn A30P

   Plasmid

   #105729

   Purpose

   Mammalian expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

   Insert

   alpha-synuclein A30P (SNCA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A30P

   Promoter

   CMV

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST53-LRRK2-Y1699C

   Plasmid

   #25048

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Tyrosine 1699 mutated to Cysteine

   Promoter

   Available Since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pX62

   Plasmid

   #46854

   Purpose

   TOP Hupki-puro2A-G245S plasmid to introduce mutant p53 to PLF ESC or MEFs by IMCE

   Depositor

   Monica Hollstein

   Article

   Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

   Insert

   attB-Puro-2A and Hupki p53 G245S (TP53 Human, Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G245S

   Promoter

   Available Since

   Aug. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX68

   Plasmid

   #46857

   Purpose

   OP Hupki-puro2A-A138V plasmid to indroduce mutant p53 to PLF ESC or MEFs by IMCE

   Depositor

   Monica Hollstein

   Article

   Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

   Insert

   attB-Puro-2A and Hupki p53 A138V (TP53 Human, Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   p53 A138V

   Promoter

   Available Since

   Aug. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. ORAI1-YFP L273D-L276D

   Plasmid

   #114182

   Purpose

   ORAI1 channel with C-terminal YFP, carrying the double mutation L273D-L276D in the STIM1 binding domain, leading to a channel gating deficiency.

   Depositor

   Nicolas Demaurex

   Article

   Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

   Insert

   ORAI1-YFP L273D-L276D (ORAI1 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   L273D/L276D

   Promoter

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. ORAI1-YFP V107M

   Plasmid

   #114180

   Purpose

   ORAI1 channel with C-terminal YFP, carrying the mutation V107M in the protein 1st transmembrane domain, responsible for a constitutive activity and associated with tubular aggregate myopathy (TAM).

   Depositor

   Nicolas Demaurex

   Article

   Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

   Insert

   ORAI1-YFP V107M (ORAI1 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   V107M

   Promoter

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-myc-his-BACH1 K52R

    Plasmid

    #17643

    Depositor

    Ronny Drapkin

    Article

    Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

    Insert

    BACH1 (BRIP1 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    The lysine residue at position 52 was changed to …

    Promoter

    Available Since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

11. HA-EglN3 H196A-pcDNA3

    Plasmid

    #22717

    Depositor

    William Kaelin

    Article

    Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

    Insert

    Egg laying Nine - 3 (EGLN3 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    mutant cDNA: point mutation at codon 196 was ins…

    Promoter

    Available Since

    Dec. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDH-EF1-FHC-POLQ-K121M

    Plasmid

    #64877

    Purpose

    lentiviral expression of human POLQ K121M mutant (a mutation in the conserved ATP-binding site of the Walker A motif in the helicase-like domain)

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    K121M, a mutation in the conserved ATP-binding si…

    Promoter

    EF1alpha

    Available Since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-FLEX-SaCas9-U6-sgGria2

    Plasmid

    #124854

    Purpose

    Mutagenesis of Gria2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria2 (Gria2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-mPCSK3 H194L

    Plasmid

    #122689

    Purpose

    Expresses mutated mouse PCSK3 in mammalian cells (histidine at position 194 mutated to leucine via site directed mutagenesis)

    Depositor

    Liming Pei

    Article

    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

    Insert

    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Histidine 194 to Leucine

    Promoter

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221 MLK1 E179K

    Plasmid

    #60541

    Purpose

    Donor vector for human MLK1 E179K mutant

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    MLK1 (MAP3K9 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    E179K

    Promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1-mPCSK3 S368A

    Plasmid

    #122680

    Purpose

    Expresses mutated mouse PCSK3 in mammalian cells (serine at position 368 mutated to alanine via site directed mutagenesis)

    Depositor

    Liming Pei

    Article

    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

    Insert

    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Serine 368 to Alanine

    Promoter

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221 MLK1 D294A

    Plasmid

    #60542

    Purpose

    Donor vector for human MLK1 D294A mutant

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    MLK1 (MAP3K9 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    D294A

    Promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pTYB11-PTBP1-RRM2CCtoSS

    Plasmid

    #89154

    Purpose

    recombinant expression of PTBP1-RRM2 C250S,C251S in e.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 2 mutant C250S, C251S (PTBP1 Human)
    

    Use

    Tags

    Intein and chitin binding domain

    Expression

    Bacterial

    Mutation

    mutations: Cys 250 to Ser, Cys 251 to Ser

    Promoter

    T7

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-FLEXfrt-SaCas9-U6-sgGria1

    Plasmid

    #124867

    Purpose

    Mutagenesis of Gria1

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria1 (Gria1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-FLEXfrt-SaCas9-U6-sgGria2

    Plasmid

    #124868

    Purpose

    Mutagenesis of Gria2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria2 (Gria2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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