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We narrowed to 10,393 results for: FIE

Showing: 4041 - 4060 of 10393 results

  3. FGG

    Plasmid
    #52031
    Purpose
    Expresses full-length Fibrinogen gamma chain precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    FGG (FGG Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Stop Codon at amino acid 454, before CD4, bio and…
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52031-plasmid-map-sequence-id-80106

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  5. FGB

    Plasmid
    #52030
    Purpose
    Expresses full-length Fibrinogen beta chain precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    FGB (FGB Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Stop Codon at amino acid 492, before CD4, bio and…
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52030-plasmid-map-sequence-id-80105

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  6. lentiCRISPRv2-sgRNAs-del-CDK5RAP2-SVA-BFP

    Plasmid
    #202824
    Purpose
    Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CDK5RAP2 gene with EF-1apha for Cas9 and BFP expression
    Depositor
    Matthew Anderson
    Article
    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:
    Insert
    two sgRNAs (each with a U6 promoter) to delete SVA within CDK5RAP2 gene
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 - two
    Available since
    July 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    202824-plasmid-map-sequence-id-399641

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  7. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #86613
    Purpose
    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Tags
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86613-plasmid-map-sequence-id-168851

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  11. BSG-bio-His

    Plasmid
    #53146
    Purpose
    Expresses full-length Basigin ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    Basigin (BSG Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    53146-plasmid-map-sequence-id-82770

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  13. LCV2_EGFP

    Plasmid
    #155098
    Purpose
    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and EGFP expression
    Depositor
    Jason Moffat
    Article
    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    155098-plasmid-map-sequence-id-307983

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  16. LCV2_mCherry

    Plasmid
    #155096
    Purpose
    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mCherry expression
    Depositor
    Jason Moffat
    Article
    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    155096-plasmid-map-sequence-id-307998

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  18. LCV2_mClover3

    Plasmid
    #155095
    Purpose
    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mClover3 expression
    Depositor
    Jason Moffat
    Article
    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    155095-plasmid-map-sequence-id-308012

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  20. CD154-His-bio

    Plasmid
    #52337
    Purpose
    Expresses full-length Tumor necrosis factor ligand superfamily member 5 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    CD154 (CD40LG Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52337-plasmid-map-sequence-id-81058

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Showing: 4041 - 4060 of 10393 results