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sg_Trp53_i4-ipUSEPR-TR657

Plasmid

#228930

Purpose

Knockdown of Trp53 in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Trp53_i4 (Trp53 sequence: GTCGCTACCTACAGCCAGGA, Mouse)

Use

Lentiviral

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Cdkn1a_i1-ipUSEPR-TR657

Plasmid

#228953

Purpose

Knockdown of Cdkn1a in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Cdkn1a_i1 (Cdkn1a Mouse)

Use

Lentiviral

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Cdkn1a_i2-ipUSEPR-TR657

Plasmid

#228954

Purpose

Knockdown of Cdkn1a in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Cdkn1a_i2 (Cdkn1a Mouse)

Use

Lentiviral

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-FRT/T0-eGFPnls-53BP1 1220-1631 WT

Plasmid

#60814

Purpose

mammalian expression vector

Depositor

Daniel Durocher

Article

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Tags

eGFP-NLS (SV40)

Expression

Mammalian

Available Since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pLX317-VRK1-K179E

Plasmid

#199644

Purpose

Expresses kinase-inactive VRK1 (K179E)

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

VRK1 (VRK1 Human)

Use

Lentiviral

Mutation

K179E

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHis-LMW_FGF2

Plasmid

#157657

Purpose

Expresses FGF2 in E.coli cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Tags

His tag

Expression

Bacterial

Mutation

changed Cystein 211,229 to serine, deleted amino …

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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DYR1A_HUMAN_D0

Plasmid

#79690

Purpose

This plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DYRK1A (DYRK1A Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
p23-NES-LwaCas13a-msfGFP-NES-Flag

Plasmid

#165070

Purpose

overexpression of LwaCas13a in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

LwaCas13a

Use

Lentiviral

Expression

Mammalian

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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Chr7_Centromere-Targeting_gRNA

Plasmid

#195129

Purpose

dual gRNA vector targeting centromere-proximal locations on Chromosome 7p and 7q in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr7 gRNA

Expression

Mammalian

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCas9-sgVRK1 #4-Blast

Plasmid

#199647

Purpose

Expresses Cas9 and sgRNA guide targeting VRK1

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A (VRK1 Human)

Use

Lentiviral

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS

Plasmid

#164565

Purpose

SARS-CoV-2 Spike protein (S-GSAS variant)

Depositor

Priyamvada Acharya

Article

Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

Insert

Spike (S-GSAS variant)

Tags

2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-285

Plasmid

#31060

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Mutation

-285 to -1 of P2 HNF4A promoter

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pT2K-LRL-IRES-Luc2

Plasmid

#109230

Purpose

For Tol2 transposon-mediated stable expression of LRL and IRES-luciferase

Depositor

Martine Roussel

Article

Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

Insert

LRL and Luc2

Expression

Mammalian

Promoter

CAGGS

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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Chr1q_Cassette-Integration_gRNA

Plasmid

#195126

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting location downstream of Chr1q_centromere-targeting_gRNA for positive-negative selection cassette integration

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr1q gRNA

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET28aAviHisCD72cCTLD

Plasmid

#129066

Purpose

generation of biotinylated mouse CD72c CTLD protein using bacteria

Depositor

Takeshi Tsubata

Article

Akatsu et al J Exp Med. 2016 Nov 14;213(12):2691-2706

Insert

CD72c (Cd72 Mouse)

Expression

Bacterial

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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AURKA_HUMAN_D0

Plasmid

#79739

Purpose

This plasmid encodes the kinase domain of AURKA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

AURKA (AURKA Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
plentiCRISPv2_FDX1-3

Plasmid

#184490

Purpose

Lentivirus all in one CRISPR vector targeting human FDX1 gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

FDX1 (FDX1 Human)

Use

Lentiviral

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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plentiCRISPv2_FDX1-1

Plasmid

#184488

Purpose

Lentivirus all in one CRISPR vector targeting human FDX1 gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

FDX1 (FDX1 Human)

Use

Lentiviral

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pαH-S-RRAR/D614G

Plasmid

#164570

Purpose

SARS-CoV-2 Spike protein with D614G mutation and Furin site restored (S-RRAR-D614G variant)

Depositor

Priyamvada Acharya

Article

Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

Insert

Spike (S-RRAR-D614G variant)

Tags

2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

Expression

Mammalian

Mutation

D614G

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pGL4.23 A82V 2014 EBOV + Mucin-Like Domain

Plasmid

#86016

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Changed alanine 82 to valine

Promoter

CMV IE

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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