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pJL50

Plasmid

#174068

Purpose

Expresses 97-3 zinc finger array fused to VP16 in S. cerevisiae. Parent plasmid for chromatin regulator library.

Depositor

Albert Keung

Article

Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

Insert

pTEF1-3NLS-97-3ZF-VP16

Tags

FLAG

Expression

Yeast

Promoter

pTEF1

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJL45

Plasmid

#174067

Purpose

Expresses CIB1 in S. cerevisiae. Includes inducible lac operon.

Depositor

Albert Keung

Article

Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

Insert

pGal1-LacO-3NLS-CIB1-tADH1

Tags

FLAG

Expression

Yeast

Promoter

pGal1

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSBbi-TIR1-tTA/Zeo

Plasmid

#171685

Purpose

constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (zeocin)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Insert

Zeocin

Expression

Mammalian

Promoter

RPBSA promoter

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJL1

Plasmid

#174070

Purpose

Expresses VP16-CIB1 in S. cerevisiae. Part of optogenetic system.

Depositor

Albert Keung

Article

Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

Insert

pGal-Olac-3xNLS-VP16-CIB1-tADH1

Tags

FLAG

Expression

Yeast

Promoter

pGal1

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pJL29

Plasmid

#174063

Purpose

Expresses for mCherry for S. cerevisiae. Contain 2 43-8 zinc-finger operators upstream of Cyc1 promoter.

Depositor

Albert Keung

Article

Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

Insert

2x43-8 ZFO-1x97-4 ZFO pCyc mCherry tCyc

Tags

FLAG

Expression

Yeast

Promoter

pCyc1

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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PLKO.1-REG1-3'UTR

Plasmid

#136052

Purpose

REG1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (ATTGTATCTCTGTAGTTTAAG)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

REG1 (ZC3H12A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-neo-lambdaN-UPF1-DEAA

Plasmid

#136007

Purpose

DEAA UPF1 inserted with lambdaN tagged on the N-terminus to use with the Tethering Assay (BoxB)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF1

Tags

LambdaN

Expression

Mammalian

Mutation

DE647AA and S743A (Please see depositor comments …

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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PLKO.1-UPF3A-3'UTR

Plasmid

#136042

Purpose

UPF3A shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GACGTAGAAACACGCAGAAAC)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF3A (UPF3A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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PLKO.1-UPF3A-CDS

Plasmid

#136043

Purpose

UPF3A shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (GCAGAAACCATTCTAAAGAAA)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF3A (UPF3A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEYRC-D1A

Plasmid

#127753

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

RFP, CFP, Divergent Orientation

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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