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pMT-MCS-NLS-BirA-2A-mCherry_Ras
Plasmid#80059PurposeMCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
LXSN-Cx43-S279D
Plasmid#60707Purposeretroviral expression of rat Cx43 S279D mutantDepositorInsertCx43 (Gja1 Rat)
UseRetroviralTagsExpressionMammalianMutationS279DPromoterLTRAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
LXSN-Cx43-S282A
Plasmid#60709Purposeretroviral expression of rat Cx43 S282A mutantDepositorInsertCx43 (Gja1 Rat)
UseRetroviralTagsExpressionMammalianMutationS282APromoterLTRAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
LXSN-Cx43-S282D
Plasmid#60711Purposeretroviral expression of rat Cx43 S282D mutantDepositorInsertCx43 (Gja1 Rat)
UseRetroviralTagsExpressionMammalianMutationS282DPromoterLTRAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S87A
Plasmid#36058DepositorInsertalpha-synuclein S87A (SNCA Human)
UseTagsExpressionBacterialMutationS87APromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJWK_VD_02
Plasmid#158103Purpose5'UTR stem-loop luciferase reporter: -30kcal/molDepositorInsert5'UTR synthetic stem-loop -30kcal/mol
UseLuciferaseTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2K930R-FF-VSV
Plasmid#139348PurposeExpression of kinase-dead TYK2-K930R missing the two tyrosines at the activation loopDepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
UseTagsVSVExpressionMammalianMutationV362F-K930R-Y1054F-Y1055FPromoterCMVAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
APCS-bio-His
Plasmid#53424PurposeExpresses full-length Serum amyloid P-component precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertAPCS (APCS Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK21054-5FF-VSV
Plasmid#139347PurposeExpression of TYK2 protein mutated in the two tyrosines (Y1054F/Y1055F) of the activation loopDepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
UseTagsExpressionMammalianMutationV362F-Y1054F-Y1055FPromoterCMVAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCR3-FLAG-Gephyrin G
Plasmid#68817PurposeGeph G domain expression in mamalian cellsDepositorInsertGephyrin (Gphn Rat)
UseTags3xFLAGExpressionMammalianMutationNILPromoterCMVAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDN-T1TCbt
Plasmid#44514DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationThree tandem tet operators (3XtetO2) downstream o…PromoterPGal1-T123 promoterAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDN-T1GZmbh
Plasmid#44522DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationThree tandem tet operators (3XtetO2) downstream o…PromoterPGal1-T123 promoterAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRS DV DNM1-EGFP
Plasmid#107794Purposedonor vector for knock-in gene editing at human DNM1 locusDepositorInsertDNM1-eGFP (DNM1 Synthetic, Human)
UseYeast-e.coli shuttle vectorTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-mRIPK1-S321E
Plasmid#115340PurposeMammalian expression of Flag-mRIPK1-S321EDepositorInsertRIPK1 (Ripk1 Mouse)
UseTagsFlagExpressionMammalianMutationS321EPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
CALCR-bio-His
Plasmid#53412PurposeExpresses full-length Calcitonin receptor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertCALCR (CALCR Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1TCbt
Plasmid#44515DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable sinceApril 30, 2013AvailabilityAcademic Institutions and Nonprofits only
