We narrowed to 15,991 results for: LEA

1. Nanog5P reporter

   Plasmid

   #16337

   Depositor

   Austin Cooney

   Article

   Gu et al Mol Cell Biol. 2005 Oct . 25(19):8507-19

   Insert

   Nanog promoter (Nanog Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Promoter

   Mouse Nanog promoter

   Available Since

   Dec. 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET26b-Granzyme A

   Plasmid

   #8823

   Depositor

   Judy Lieberman

   Article

   Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

   Insert

   Human Granzyme A (GZMA Human)
   

   Tags

   (His)6 and Enterokinase site

   Expression

   Bacterial

   Mutation

   it mature enzyme.

   Available Since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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3. pExpreS2-2

   Plasmid

   #175362

   Purpose

   G418R backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

   Depositor

   ExpreS2ion Biotechnologies

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Expression

   Insect

   Promoter

   Fused Actin-HSP70

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-FLAG-PGRMC1-1-170

   Plasmid

   #173467

   Purpose

   PGRMC1 lacking the extreme C-terminus

   Depositor

   Bill Tsai

   Article

   Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

   Insert

   PGRMC1 (PGRMC1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deleted for amino acids 171-194

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSC234

   Plasmid

   #216357

   Purpose

   xhNup93-Nb3t (+2Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xhNup93-Nb3t (+2Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSJ2039 (pRS316-NOP1pr-GFP1-10-SCS2TM)

   Plasmid

   #86418

   Purpose

   ONM/ER split GFP1-10

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   NOP1pr-GFP1-10-Scs2TM
   

   Tags

   GFP1-10

   Expression

   Yeast

   Promoter

   NOP1

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-mCherry-4A/4B-CAAX

   Plasmid

   #87699

   Purpose

   Express mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   mCherry-4A/4B-CAAX
   

   Expression

   Mammalian

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOCC5

   Plasmid

   #118863

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. AW01_pU.CAG.eSpCas9(1.1).rBGpA

   Plasmid

   #199255

   Purpose

   Expression construct encoding the high specificity eSpCas9(1.1) nuclease

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

   Insert

   High specificity eSpCas9(1.1) nuclease
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   K848A K1003A R1060A

   Promoter

   CAG promoter

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Membrane mNeonGreen

    Plasmid

    #198057

    Purpose

    Plasma membrane localizing mNeonGreen using Human LCK proto-oncogene sequence

    Depositor

    Amro Hamdoun

    Insert

    LCK membrane mNeonGreen (LCK A.victoria, Human)
    

    Use

    Synthetic Biology; Sea urchin, mammal, zebrafish

    Tags

    mNeonGreen

    Mutation

    Tandem repeat of N-terminal LCK membrane targetin…

    Promoter

    sp6

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cas12j-8-crRNA

    Plasmid

    #194967

    Purpose

    The plasmid expresses Cas12j-8-crRNA expression elements.

    Depositor

    Yongming Wang

    Article

    Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Membrane EGFP

    Plasmid

    #198056

    Purpose

    Plasma membrane localizing EGFP using Human LCK proto-oncogene sequence

    Depositor

    Amro Hamdoun

    Insert

    LCK membrane EGFP (LCK A. victoria, Synthetic, Human)
    

    Use

    Sea urchin, mammal, zebrafish

    Tags

    EGFP

    Mutation

    Tandem repeat of N-terminal LCK membrane targetin…

    Promoter

    sp6

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLS.402

    Plasmid

    #187202

    Purpose

    Expresses retron Eco1 RT

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Insert

    Retron Eco1 Reverse Transcriptase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    mphR

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRG713_lexO-HO_LexA-TF_URA3MX

    Plasmid

    #154821

    Purpose

    Inducible expression of HO endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    HO endonuclease (HO Budding Yeast)
    LexA-ER-B112
    

    Tags

    FLAG

    Expression

    Yeast

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry-Vinculin-N-21

    Plasmid

    #55160

    Purpose

    Localization: Focal Adhesions, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

    Insert

    Vinculin (VCL Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA-Ran-Q69L-mRFP1-polyA

    Plasmid

    #104560

    Purpose

    Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran (Ran Mouse)
    

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    Q69L

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDG04439

    Plasmid

    #216365

    Purpose

    xhNup133-Nb2t (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup133-Nb2t (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-ninein C-ter

    Plasmid

    #73523

    Purpose

    Encodes the C-terminus of mouse ninein (residues 1874-2113) with an EGFP tag at its N-terminus

    Depositor

    Michel Bornens

    Article

    Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

    Insert

    ninein C-ter (residues 1874-2113) (Nin Mouse)
    

    Tags

    EGFP tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2016

    Availability

    Academic Institutions and Nonprofits only

19. 8xGliBS-IVS2-mCherry-NLS-Odc1-polyA-Tol2

    Plasmid

    #84603

    Purpose

    Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

    Depositor

    James Chen

    Article

    Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

    Insert

    mCherry
    

    Use

    Zebrafish transgenesis

    Tags

    ODC1 destabilizing element

    Promoter

    8xGliBS-delta-crystallin minimal promoter

    Available Since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3NES-CS(G)-tTA-CS(G)-3NES

    Plasmid

    #137836

    Purpose

    Soluble tTA transcription factor flanked by N and C terminal TEV protease G cleavage sequences and 3 nuclear export sequences

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    3NES-CD(G)-tTA-CS(G)-3NES
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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