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We narrowed to 4,449 results for: erf

Showing: 4081 - 4100 of 4449 results

  3. pET28a-CohIII-CBM-HIS-ybbR

    Plasmid
    #60866
    Purpose
    Characteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tag
    Depositor
    Hermann Gaub
    Article
    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1
    Inserts
    CohIII
    CBM
    Use
    Tags
    6xHIS, HRV 3C, and ybbR
    Expression
    Bacterial
    Mutation
    Cysteine 63 to Serine in CBM module
    Promoter
    T7
    Available since
    Nov. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    60866-plasmid-map-sequence-id-98102

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  4. pET28a-HIS-Xyl-Xmod-DocIII

    Plasmid
    #60865
    Purpose
    Characteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120C
    Depositor
    Hermann Gaub
    Article
    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1
    Inserts
    Xyl
    Xmod-DocIII
    Use
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    Threonine 120 to Cysteine in Xylanase domain
    Promoter
    T7
    Available since
    Nov. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    60865-plasmid-map-sequence-id-98100

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  6. pLV-dCas9-KRAB-PGK-HygR

    Plasmid
    #83890
    Purpose
    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.
    Depositor
    Charles Gersbach
    Article
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    Use
    CRISPR and Lentiviral
    Tags
    Flag
    Expression
    Mutation
    D10A and H840A
    Promoter
    Human Ubiquitin C Promoter and mouse phosphoglyce…
    Available since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    83890-plasmid-map-sequence-id-240902

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  7. pIA33

    Plasmid
    #120803
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Inserts
    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Base-pairing region targets red fluorescent prote…
    Promoter
    Pgdh and Pxyl
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120803-plasmid-map-sequence-id-235316

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  9. pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139094
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139094-plasmid-map-sequence-id-273891

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  16. pGEX:PGRMC1

    Plasmid
    #136062
    Purpose
    Bacterial expression of pGEX:PGRMC1 to perform a GST-pulldown assay and identify new proteins that interact with GST-PGRMC1 in the human endometrial stroll cells.
    Depositor
    Francisco Domínguez Hernández
    Article
    Salsano et al Fertil Steril. 2020 May;113(5):1050-1066
    Insert
    Progesterone receptor membrane component 1 (PGRMC1 Human)
    Use
    Tags
    GST-PGRMC1
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    136062-plasmid-map-sequence-id-272028

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  17. TFORF1377

    Plasmid
    #141822
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF148 (ZNF148 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141822-plasmid-map-sequence-id-397942

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  18. pCIBN-tagBFP-hTRF2

    Plasmid
    #103803
    Purpose
    BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-tagBFP-hTRF2 (CIB1, TERF2 Human, Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hTRF2: deletion of amino acids 1-42 and 476 compa…
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103803-plasmid-map-sequence-id-199226

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Showing: 4081 - 4100 of 4449 results