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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,229 results for: sgRNA

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Showing: 4081 - 4100 of 9229 results

  4. pXPR_016

    Plasmid
    #107143
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Hygro resistance from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    David Root
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Available since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    107143-plasmid-map-sequence-id-205293

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  8. pUC57-Amp_CRISPIE donor B4

    Plasmid
    #172845
    Purpose
    CRISPIE donor B4 (Zhong et al, eLife 2021), mNeonGreen translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPIE designer exon (phase 0-0) encoding mNeonGreen
    Use
    CRISPR
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172845-plasmid-map-sequence-id-340978

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  13. pGMC00010 (aka pRC0495)

    Plasmid
    #195295
    Purpose
    Lenti-CRISPR-V2-RFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/RFP
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195295-plasmid-map-sequence-id-387224

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  14. pDual_dsCas9_Blast

    Plasmid
    #214681
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two empty sgRNA cloning sites
    Depositor
    Kevin Janes
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214681-plasmid-map-sequence-id-428811

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  15. pET-FLAG-B-eSpCas9

    Plasmid
    #126772
    Purpose
    Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    T7
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126772-plasmid-map-sequence-id-410600

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Showing: 4081 - 4100 of 9229 results