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mCherry-S-SpyTag

Plasmid

#243786

Purpose

Expresses multifunctional construct enabling the YES-gated release of mCherry from materials in response to sortase eSrtA(4S9) treatment. Construct contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mCherry

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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TVMV Protease-mCherry

Plasmid

#243794

Purpose

Encodes for mammalian expression of TVMV protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

TVMV protease

Expression

Mammalian

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-A-SpyTag

Plasmid

#243769

Purpose

Expresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) treatment. Construct contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-S-SpyTag

Plasmid

#243770

Purpose

Expresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) treatment. Construct contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-T-SpyTag

Plasmid

#243771

Purpose

Expresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to TEV treatment. Construct contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-A OR S-SpyTag

Plasmid

#243772

Purpose

Expresses multifunctional construct enabling the OR-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR sortase eSrtA(4S9) treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-A OR T-SpyTag

Plasmid

#243773

Purpose

Expresses multifunctional construct enabling the OR-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR TEV treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-S OR T-SpyTag

Plasmid

#243774

Purpose

Expresses multifunctional construct enabling the OR-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) OR TEV treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-A AND T-SpyTag

Plasmid

#243776

Purpose

Expresses multifunctional construct enabling the AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND TEV treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag and SsrA

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-AHR-shRNA2

Plasmid

#166908

Purpose

Lentivirus for inducible knockdown of human AHR

Depositor

Roland Friedel

Article

Halawani et al bioRxiv 2023.11.04.565649. doi: https://

Insert

AHR shRNA

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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EBFP2-PIPKγ87-D253A

Plasmid

#242825

Purpose

Encodes human PIPKγ87-D253A and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)

Expression

Mammalian

Mutation

D253A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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EBFP2-PIPKγ87

Plasmid

#242824

Purpose

Encodes wildtype human PIPKγ87 and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-GFP

Plasmid

#240128

Purpose

Expresses GFP in mammalian cells

Depositor

Timothy Ley

Article

Kramer et al J Clin Invest. 2025 Aug 7:e195929. doi:

Insert

GFP

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pET-21_MBP-VidN

Plasmid

#241013

Purpose

VidN construct that can be bacterially expressed, used to demonstrate intein splicing reaction.

Depositor

Cole DeForest

Article

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

MBP-VidN

Tags

MBP-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19::ZmUBI-RWT4Ca_K217R/K327R

Plasmid

#237511

Purpose

Protoplast expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca_K217R/K327R

Expression

Plant

Mutation

Mutations at kinase active sites (K217R, K327R)

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF_AaNHis_RbcL

Plasmid

#229517

Purpose

Expresses Anthoceros agrestis Rubisco large (N terminus hexahistidine)

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Insert

Rubisco large subunit

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19::ZmUBI-RWT4Ca_Reg2

Plasmid

#237513

Purpose

Protoplast expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca_Reg2

Expression

Plant

Mutation

Removed binding region 2

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:His-MBP-PKD

Plasmid

#237521

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4_pseudokinase

Expression

Bacterial

Mutation

Pseudokinase region of RWT4

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGADT7-RWT4Ca+C-Tail

Plasmid

#237526

Purpose

Yeast two-hybrid

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca+C-Tail

Expression

Yeast

Mutation

Add C-terminal extension

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:His-MBP-KD

Plasmid

#237520

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4_kinase

Expression

Bacterial

Mutation

Kinase region of RWT4

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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