We narrowed to 4,240 results for: pes

1. pAAV-CAG-DIO-ECS-dSpyCatcher003

   Plasmid

   #234963

   Purpose

   Cre-dependent ECS-facing destabilized SpyCatcher003 (facing the extracellular space)

   Depositor

   Hajime Hirase

   Article

   Vittani et al bioRxiv 2025.03.18.643859

   Insert

   ECS-dSC
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCOA-Leu-FvPPT1

   Plasmid

   #179923

   Purpose

   Expression vector for heterologous expression of a polyketide synthase in S. cerevisiae. Carries and empty insertion site and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1

   Depositor

   Jens Laurids Sørensen

   Article

   Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

   Insert

   FvPPT1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Codon optimized for expression in Saccharomyces c…

   Promoter

   Yarrowia lipolytica EXP1

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL4.15_pT-REX17

   Plasmid

   #195497

   Purpose

   Firefly luciferase promoter expression vector driven only by the promoter element of human lncRNA T-REX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   Promoter region of lncRNA T-REX17
   

   Use

   Tags

   luc2-hPEST

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. H2B-mNeonGreen_FRB(DmrC)_IRESpuro2

   Plasmid

   #182918

   Purpose

   Expesses H2B-mNeonGreen_FRB(DmrC). Can be used to target FKBP(DmrA) tagged proteins to H2B

   Depositor

   Daniel Gerlich

   Article

   Cuylen-Haering et al Nature. 2020 Nov;587(7833):285-290. doi:

   Insert

   pH2B (H2BC16P Human)
   

   Use

   Tags

   mNeonGreen-FRB(DmrC)

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEP4-CH111-QES.c13.L544Y-754*

   Plasmid

   #123264

   Purpose

   Mammalian expression plasmid for Env from the CH111 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (AD8) Env
   

   Use

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; Premature stop co…

   Promoter

   CMV

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCEP4-AD8-QES.i08.c04-754*

   Plasmid

   #123258

   Purpose

   Mammalian expression plasmid for Env from the AD8 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (AD8) Env
   

   Use

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; Premature stop co…

   Promoter

   CMV

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCEP4-SHIV327C-HuA10-QES.i09.c07

   Plasmid

   #123269

   Purpose

   Mammalian expression plasmid for Env from the SHIV327C-Hu A10 strain; QES mutant for enhanced presentation of quaternary epitopes

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   SHIV327C-Hu A10 Env
   

   Use

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; Mutations V208M, …

   Promoter

   CMV

   Available since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a_6HT-TBP6.7(R52A)

   Plasmid

   #112729

   Purpose

   52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R52A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R52A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. sgFFL_d23

   Plasmid

   #59892

   Purpose

   synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with two seed sites mutated

   Depositor

   Georg Seelig

   Article

   Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.

   Insert

   mCherry with intron containing the mouse mir-124–3
   

   Use

   Tags

   PEST and VAMP 3 UTR

   Expression

   Mammalian

   Mutation

   VAMP 3 UTR--3rd and fourth seed site removed

   Promoter

   doxycycline (Dox)-inducible promoter (CMV/TO)

   Available since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgFFL_d3

    Plasmid

    #59891

    Purpose

    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with one seed site mutated.

    Depositor

    Georg Seelig

    Article

    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.

    Insert

    mCherry with intron containing the mouse mir-124–3
    

    Use

    Tags

    PEST and VAMP 3 UTR

    Expression

    Mammalian

    Mutation

    VAMP 3 UTR--3rd seed site removed

    Promoter

    doxycycline (Dox)-inducible promoter (CMV/TO)

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti6/V5-DEST-SPAG5

    Plasmid

    #31217

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    SPAG5 (SPAG5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Contains a one nucleotide deletion at C-term that…

    Promoter

    Available since

    Aug. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-hSyn-SOUL-P2A-TdTomato-WPRE-hGH-polyA

    Plasmid

    #177576

    Purpose

    Expresses SOUL in neuronal cell types

    Depositor

    Guoping Feng

    Article

    Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1

    Insert

    SOUL
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-LUC2CP/intron/ARE

    Plasmid

    #62858

    Purpose

    splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

    Depositor

    Gideon Dreyfuss

    Article

    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

    Insert

    Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    insertion of chimeric intron at nueotide poclsiti…

    Promoter

    CMV

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCR8/GW_TOPO_Cdk12_Nterm_FlagHa

    Plasmid

    #127177

    Purpose

    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

    Depositor

    Phil Sharp

    Article

    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

    Insert

    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    

    Use

    Entry vector with transgene for gateway cloning

    Tags

    Flag- HA- Tandem Epitopes

    Expression

    Mutation

    Promoter

    None

    Available since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-double floxed-ClopHensorN-WPRE-HGHpA

    Plasmid

    #193728

    Purpose

    Cre-activated AAV expression of ClopHensorN for measuring intracellular chloride and pH in genetically-defined cell types

    Depositor

    Colin Akerman

    Article

    Calin et al iScience. 2023 Mar 9;26(4):106363. doi:

    Insert

    ClopHensorN
    

    Use

    AAV

    Tags

    Strep-II (N terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHiFi Cas9-2×sgRNA (empty, donor)

    Plasmid

    #162277

    Purpose

    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.

    Depositor

    Kazuhisa Nakayama

    Article

    Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi

    Insert

    HiFi Cas9
    

    Use

    CRISPR

    Tags

    BPNLS and FLAG tag

    Expression

    Mammalian

    Mutation

    SpCas9 (R691A)

    Promoter

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. hZMPSTE24-3FLAG_pQCXIP

    Plasmid

    #89759

    Purpose

    Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.

    Depositor

    Martin Dorf

    Article

    Fu et al J Exp Med. 2017 Feb 28. pii: jem.2016127

    Insert

    Homo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)
    

    Use

    Retroviral; Bicistronic expression: puromycin re…

    Tags

    9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…

    Expression

    Mammalian

    Mutation

    Y253H in ZMPSTE24 (see depositor comments below)

    Promoter

    CMV

    Available since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCEP4-BG505.SOSIP-gp160-QES.i05.c06

    Plasmid

    #123276

    Purpose

    Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations); QES mutant for enhanced presentation of quaternary epitopes

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BG505) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; SOSIP mutations; …

    Promoter

    CMV

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST-mCherry-LacR-BRCA1 1366-1437

    Plasmid

    #71118

    Purpose

    mammalian expession of mCherry-LacR-BRCA1 1366-1437

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    contains amino acids 1366-1437 of BRCA1

    Promoter

    CMV/TO

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. sgFFL_7x_Bulge

    Plasmid

    #59894

    Purpose

    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with seven bulge target sites

    Depositor

    Georg Seelig

    Article

    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.

    Insert

    mCherry with intron containing the mouse mir-124–3
    

    Use

    Tags

    PEST and VAMP 3 UTR

    Expression

    Mammalian

    Mutation

    VAMP 3 UTR--has seven bulge targets (fully comple…

    Promoter

    doxycycline (Dox)-inducible promoter (CMV/TO)

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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