We narrowed to 8,514 results for: sgrna

1. Level 1 P3 TaU6 guide acceptor

   Plasmid

   #165599

   Purpose

   GoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmid

   Depositor

   John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

   Article

   Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

   Insert

   TaU6 promoter::LacZ::sgRNA scaffold
   

   Use

   CRISPR and Synthetic Biology; Sgrna acceptor with…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMM136

   Plasmid

   #127217

   Purpose

   BeYDV replicon with constitutive Luc expression, MP-delta, sgRNA targeting PDS

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   Luc, MP-delta, sgRNA
   

   Use

   Tags

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMM134

   Plasmid

   #127215

   Purpose

   BeYDV replicon with constitutive Luc expression, IPT, sgRNA targeting PDS

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   Luc, IPT, sgRNA
   

   Use

   Tags

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFD5-frame_selector_0,1,2

   Plasmid

   #131152

   Purpose

   Drosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-in

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   CRISPaint frame selector sgRNAs 0,1,2
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dU6:3

   Available since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS143

   Plasmid

   #122377

   Purpose

   CRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

   Depositor

   Rebecca Shapiro

   Article

   Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-Cas9

   Plasmid

   #64237

   Purpose

   expresses codon optimized Cas9 in zebrafish

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Insert

   codon optimized Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMCRi

   Plasmid

   #207524

   Purpose

   Plasmid for CRISPRi; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR

   Depositor

   Pablo Ivan Nikel

   Article

   Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

   Insert

   xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR
   

   Use

   Pseudomonas vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. gRNA Cloning Vector Bbs I ver. 2

   Plasmid

   #85586

   Purpose

   An empty sgRNA expression vector. sgRNA sequence can be cloned into its Bbs I site. Its gRNA scaffold has efficient ver. 2 structure.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al Sci Rep. 2016 Jul 28;6:30485. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pChimera

   Plasmid

   #61476

   Purpose

   Vector for conventional cloning that contains AtU6-26 promoter and sgRNA backbone

   Depositor

   Holger Puchta

   Article

   Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

   Insert

   U6-26:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   U6-26

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMM146

    Plasmid

    #127218

    Purpose

    BeYDV replicon with constitutive Luc expression, BBM, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, BBM, sgRNA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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