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ptCMV-153/47-VR-NG

Plasmid

#50704

Purpose

Final destination vector

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

TALEN-FokI nuclease backbone

Use

TALEN

Tags

Flag

Promoter

CMV

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCRII-TOPO CMV-cGFP-bGH Poly(A) Sense

Plasmid

#46835

Purpose

Express cGFP transcript ending in a poly(A) tail [using the bGH poly(A) signal]

Depositor

Phil Sharp

Publication

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Coral Green Fluorescent Protein (cGFP)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFLAG-EEF1D (pcDNA 3.1+) LG3-1

Plasmid

#37364

Depositor

David Litchfield

Publication

Gyenis et al J Proteome Res. 2011 Nov 4;10(11):4887-9

Insert

EEF1D (EEF1D Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

July 17, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-hST3GAL6-RFP

Plasmid

#22923

Depositor

Edward Callaway

Publication

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

human type 2 lactosamine alpha-2,3-sialyltransferase promoter driving DsRedExpress (ST3GAL6 Human)

Use

AAV

Expression

Mammalian

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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KRAB-dSpCas9

Plasmid

#205248

Purpose

In vitro transcription plasmid for production of KOX1 KRAB-dSpCas9 mRNA for transcriptional repression (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #85449.

Depositor

Rasmus Bak

Publication

Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

Insert

KRAB-dSpCas9

Use

CRISPR

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_WiChR_TS_mScarlet_ER

Plasmid

#205997

Purpose

High efficient, K+ selective channelrhodopsin for the inhibition of neurons

Depositor

Peter Hegemann, Johannes Vierock

Publication

Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

Has service

AAV9

Insert

Wobblia Inhibitory Channelrhodopsin

Use

AAV

Tags

TS, mScarlet, ER

Promoter

hSyn

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MAC-ANPEP

Plasmid

#172418

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Aminopeptidase N (AP-N) (CD antigen CD13) (ANPEP Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-hSyn-OsTIR1(WT)

Plasmid

#140729

Purpose

OsTIR1(WT)-P2A-mAID-EGFP-NES

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(WT)-P2A-mAID-EGFP-NES

Use

AAV

Expression

Mammalian

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM65879

Plasmid

#153214

Purpose

Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and Bar selection cassette

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3.1/NDUFS3/myc-His

Plasmid

#127487

Purpose

Expresses myc-tagged NDUFS3 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Publication

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS3 (NDUFS3 Human)

Tags

myc-His

Expression

Mammalian

Promoter

CMV

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2/myc-His

Plasmid

#127486

Purpose

Expresses myc-tagged NDUFS2 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Publication

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Promoter

CMV

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-sgRNA(lib)-puro

Plasmid

#119976

Purpose

Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Wensheng Wei

Publication

Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EF1a_FOXA3_P2A_Hygro_Barcode

Plasmid

#120440

Purpose

Barcoded lentiviral vector to express FOXA3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

FOXA3 (FOXA3 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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AAEL006511-Cas9

Plasmid

#100580

Purpose

The promoter of AAEL006511 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

Depositor

Omar Akbari

Publication

Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

Inserts

AAEL006511-hspcas9-GFP
Opie2-dsRed

Use

CRISPR

Tags

GFP and dsRED

Expression

Insect

Promoter

AAEL006511 and Opie2

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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delta guaB

Bacterial strain

#113653

Purpose

Base strain that the pDCAF plasmids (Addgene #113646-113652) must be in.

Depositor

Jeffrey Barrick

Publication

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Bacterial resistance

Kanamycin

Available since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits
pAS166

Plasmid

#194489

Purpose

43-8 Op (Part 2)

Depositor

Ahmad Khalil

Publication

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

43-8 Op (Part 2)

Use

Synthetic Biology

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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SCP2-pUC119

Plasmid

#21951

Depositor

James Kadonaga

Publication

Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

Insert

SCP2 (super core promoter 2)-pUC119

Use

A puc derivative - a cloning vector

Available since

Feb. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pBS cGli2 (CT#673)

Plasmid

#13955

Depositor

Cliff Tabin

Publication

Schweitzer et al Mech Dev. 2000 Nov . 98(1-2):171-4.

Insert

Gli2 (GLI2 Chicken)

Expression

Bacterial

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pBS cGli3 (CT#34)

Plasmid

#13953

Depositor

Cliff Tabin

Publication

Schweitzer et al Mech Dev. 2000 Nov . 98(1-2):171-4.

Insert

Gli3 (GLI3 Chicken)

Expression

Bacterial

Available since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-DIO-GRAB_CRF1.0

Plasmid

#208661

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent manner

Depositor

Yulong Li

Publication

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0

Use

AAV

Promoter

hSyn

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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