We narrowed to 9,802 results for: otos

1. pME-mScarlet3-Giantin (JDW 1356)

   Plasmid

   #224502

   Purpose

   Gateway compatible middle entry clone containing a V5-tagged mScarlet fused to Giantin (For visualizing the Golgi)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   mScarlet3-Giantin
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-mCherry-3’UTR of β-actin-(F30-2xdBroccoli)6

   Plasmid

   #190162

   Purpose

   Expresses 24 Broccoli-tagged beta-actin mRNA in mammalian cells

   Depositor

   Samie Jaffrey

   Article

   Li et al Angew Chem Int Ed Engl. 2020 Mar 9;59(11

   Insert

   mcherry_(3'UTR, No Actin)_24Broccoli
   

   Use

   Tags

   24×Broccoli

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRXIP-GFP-PI4KB

   Plasmid

   #221759

   Purpose

   Stable expression of GFP-PI4KB in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   PI4KB (PI4KB Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. 7Gli:GFP

   Plasmid

   #110494

   Purpose

   reporter of Hedgehog signaling activity

   Depositor

   Michael Lewis

   Article

   Li et al PLoS Genet. 2016 May 19;12(5):e1006054.

   Insert

   7 repeats of the Gli binding site GAACACCCA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   7 repeats of Gli biding site

   Available since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. hSyn1-2xNLS-mTurquoise2

   Plasmid

   #118025

   Purpose

   An AAV genome that expresses nuclear localized mTurquoise2 from the hSyn1 promoter

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   2xNLS-mTurquoise2
   

   Use

   AAV

   Tags

   NLS

   Expression

   Mutation

   Promoter

   hSyn1

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-JEDI-1P-WPRE

   Plasmid

   #202613

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSyn

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   JEDI-1P
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.hSynapsin.(cyto).iATPSnFR2.A95A.A119L.HaloTag

   Plasmid

   #209665

   Purpose

   Expression of iATPSnFR2, low affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.A95A.A119L.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   A95A.A119L

   Promoter

   hSyn

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)-mTagBFP2-MRTF-A

   Plasmid

   #182061

   Purpose

   Mammalian expression vector encoding myocardin-related transcription factor A tagged with mTagBFP2.

   Depositor

   Jin Zhang

   Article

   Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

   Insert

   mTagBFP2-MRTF-A (MRTFA Human)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-EGFP-Rac1-wt

   Plasmid

   #12980

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   Rac1 (RAC1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDF0118 DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon

    Plasmid

    #172503

    Purpose

    Encodes the DisCas7-11 protein only for bacterial recombinant protein expression for purification

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBJB20 - eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

    Plasmid

    #158967

    Purpose

    Ubiquitous expression of mtON in C. elegans

    Depositor

    Andrew Wojtovich

    Article

    Berry et al EMBO Rep. 2020 Apr 3;21(4):e49113. doi:

    Insert

    eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    eft-3

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. mTagRFP-T-H2B-6

    Plasmid

    #58019

    Purpose

    Localization: Nucleus/Histones, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. mApple-Annexin(A4)-12

    Plasmid

    #54867

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Annexin (A4) (ANXA4 Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199363

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 MEKK1 WT

    Plasmid

    #12181

    Depositor

    Gary Johnson

    Article

    Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

    Insert

    MEKK1 (Map3k1 Mouse)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    V1467L

    Promoter

    Available since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAD/CMV/V5-DEST-Cdc42-2G

    Plasmid

    #68812

    Purpose

    FRET-based biosensor reporting on endogenous Cdc42 activation (for Adenovirus production)

    Depositor

    Olivier Pertz

    Article

    Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

    Insert

    Cdc42 activation reporter
    

    Use

    Adenoviral

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. p5E-mmCdh5pro (JDW 1239)

    Plasmid

    #224478

    Purpose

    Gateway compatible 5' entry clone containing the Murine Cdh5 promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Cdh5 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. hGATA-1 WT

    Plasmid

    #118352

    Purpose

    This plasmid expresses human GATA-1 WT.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hGATA-1 (GATA1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTS97 pHAGE2 CMVtetO2 GFP-22xGS-SUMOstar-MCS

    Plasmid

    #199350

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOstar-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP-SUMOstar

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYES1L-URA

    Plasmid

    #84301

    Purpose

    E.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.

    Depositor

    George Church

    Article

    Ostrov et al Science. 2016 Aug 19;353(6301):819-22. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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