We narrowed to 4,353 results for: Abo
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Plasmid#206955PurposeMammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~6% surface decoration)DepositorInsertQtEnc, antiGFP-intrabody
ExpressionMammalianAvailable SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
1M-Qt-FLAG-TGA-rt20s-antiGFP
Plasmid#206953PurposeMammalian expression of 1M-Qt EMcapsulins targeting GFP with stoichiometry control (~20% surface decoration)DepositorInsertMmMT3, QtEnc, antiGFP-intrabody
ExpressionMammalianAvailable SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHW539
Plasmid#198811PurposeDominant negative form of Protein Kinase A (PKA). Lowers PKA activity in targeted neurons.DepositorInsert15xUAS::PKA(DN)-SL2-GFP::let-858 3'UTR
ExpressionWormMutationG310D mutation to abolish the binding of cAMP of …Available SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70DAmt1-DadR/DadS-Pdadh-DadhABC(R506S, A2)
Plasmid#191631PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain (R506S mutation) with transcriptional regulators DadR/DadSDepositorInsertDopamine dehydroxylase (R506S mutation, inactive form)
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBY011-AMN1ko
Plasmid#183099PurposeS. cerevisiae Sigma1278b AMN1 gene knock out plasmid with KanMX selection marker.DepositorInsertAMN1 (left homology arm) - KanMX - AMN1 (right homology arm) (AMN1 Budding Yeast)
TagsKanMXExpressionBacterial and YeastMutationonly includes external sequences (504 bp each) as…PromoterURA3, TEF1Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-sgPLXNB2
Plasmid#86151PurposeCas9/CRISPR plasmid for cut in human PLXNB2 gene exon 3DepositorInsertPlexin-B2 (PLXNB2 Human)
UseCRISPRAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSHDY*in_PpetE:BCD2:AgBIS:Flag
Plasmid#133971PurposeHeterologous, copper-inducible expression of (E)-α-bisabolene synthase from Abies grandis (AgBIS) in Synechocystis sp. PCC 6803. The AgBIS CDS is fused to a C-termial Flag-tag.DepositorInsertAgBIS:Flag
UseSynthetic BiologyTagsFlagExpressionBacterialMutationinserted gene is codon optimized for Synechocyst…PromoterPpetE:BCD2Available SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSHDY*in_PpetE:BCD2:MrBBS:Strep
Plasmid#133973PurposeHeterologous, copper-inducible expression of (-)-α- bisabolol synthase from Matricaria recutita (MrBBS) in Synechocystis sp. PCC 6803. The MrBBS CDS is fused to a C-termial StrepII-tag.DepositorInsertMrBBS:StrepII
UseSynthetic BiologyTagsStrepIIExpressionBacterialMutationinserted gene is codon optimized for Synechocyst…PromoterPpetE:BCD2Available SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB6-Lrig1-T2A-tdTomato-FNF-TK
Plasmid#134320PurposeB6J Lrig1-T2A-tdTomato allele targeting vector, low copy numberDepositorAvailable SinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB6-Cdk6-T2A-td-sfGFP-FNF-TK
Plasmid#134321PurposeB6J Cdk6-T2A-td-sfGFP allele targeting vector, low copy numberDepositorInsertCdk6 (Cdk6 Mouse)
UseMouse TargetingTagsC-terminal fusion of T2A-tandem dimer-superfolder…Available SinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA/Bmal F423R/V435R/W427R
Plasmid#47351PurposePcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003DepositorAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
ppyCAG_RNaseH1_D210N
Plasmid#111904PurposeExpress D210N mutated RNASEH1 which abolishes the catalytic activity of RNASEH1DepositorAvailable SinceJuly 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Mito-PyronicSF/pCMV-myc-mito
Plasmid#124813PurposeHighly resposive GFP-based pyruvate nanosensor to explore mitochondrial metabolism.DepositorInsertPyronicSF
TagsMitochondrial Sequence SignalExpressionMammalianPromoterCMVAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER dCas9-TET1CDMut
Plasmid#101920PurposeThe dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.DepositorInsertdCas9-TET1CD Mut
UseLentiviralExpressionMammalianMutationMutated TET1 catalytic domainAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgR26
Plasmid#127376PurposeUsed for contransfection with pR26- and pR26-CMVconst-derived constructs to mediate CRISPR/Cas9 genomic insertion into the murine ROSA26 safe harbor locus.DepositorInsertROSA26 (Gt(ROSA)26Sor Mouse)
UseCRISPR and Mouse TargetingAvailable SinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-H2BmGreenLantern
Plasmid#177332PurposeTo express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.DepositorInsertH2B (Hist1h2bq Rat)
UseAAVTagsmGreenLanternExpressionMammalianMutationThis fusion protein was optimized to the Human co…PromoterCAGAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-ACTA2_gRNA1-SpCas9-T2A-GFP
Plasmid#126706Purposeto express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequenceDepositorInsertACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
UseCRISPRAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
VE-cad-eGFP Donor plasmid
Plasmid#92309PurposeKnock eGFP into human VE-cad locusDepositorInsertVEcad Homologous Recombination Arms (CDH5 Human)
UseCRISPRExpressionMammalianMutationabout 30 a.a. truncated at end of proteinAvailable SinceAug. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV hSYN DIO mGluR1-myc-FLAG
Plasmid#166227PurposeAAV expressing cre-dependent mGluR1-myc-FLAGDepositorInsertmetabotropic glutamate receptor 1 (Grm1 Mouse)
UseAAV and Cre/LoxTagsMyc and FLAGExpressionMammalianPromoterhuman SynapsinAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-PLXNB2
Plasmid#86237PurposeLentivirus for expression of human Plexin-B2DepositorInsertPlexin-B2 (PLXNB2 Human)
UseLentiviralExpressionMammalianMutationfull length PLXNB2, CRISPR resistantPromoterhuman PGK promoterAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only