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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,824 results for: nls

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Showing: 4161 - 4180 of 18824 results

  1. MSP2283

    Plasmid
    #70702
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70702-plasmid-map-sequence-id-127769

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  3. pCR1056

    Plasmid
    #71824
    Purpose
    Express Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLS
    Depositor
    Jacob Corn
    Publication
    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    T7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
    Use
    CRISPR
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    71824-plasmid-map-sequence-id-131017

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  4. pLenti-D156R LbCas12a-8xNLS

    Plasmid
    #248896
    Purpose
    Lentiviral vector expressing human codon-optimized D156R LbCas12a with 8xNLS
    Depositor
    Michael Aregger
    Insert
    D156R-LbCas12a-8xNLS-Myc-NeoR
    Use
    CRISPR and Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    D156R
    Promoter
    eIFa
    Available since
    Oct. 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    248896-plasmid-map-sequence-id-498938

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  5. pLenti-hyper LbCas12a-8xNLS

    Plasmid
    #248897
    Purpose
    Lentiviral vector expressing human codon-optimized hyper LbCas12a with 8xNLS
    Depositor
    Michael Aregger
    Insert
    hyper-LbCas12a-8xNLS-Myc-NeoR
    Use
    CRISPR and Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    D156R/D235R/E292R/D350R
    Promoter
    eIFa
    Available since
    Oct. 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    248897-plasmid-map-sequence-id-498940

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  6. plenti-ALFA-SpdCas9-2xNLS

    Plasmid
    #248898
    Purpose
    Lentiviral vector expressing human codon-optimized, ALFA-tagged SpdCas9 (deactivated DNAse domain)
    Depositor
    Michael Aregger
    Insert
    5xALFA-FLAG-SpdCas9-2xNLS-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A/H840A
    Promoter
    eIFa
    Available since
    Oct. 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    248898-plasmid-map-sequence-id-498941

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  7. plenti-ZIM3-SpdCas9-2xNLS

    Plasmid
    #248901
    Purpose
    Lentiviral vector expressing human codon-optimized SpdCas9 (deactivated DNAse domain) fused to ZIM3 KRAB domain
    Depositor
    Michael Aregger
    Insert
    ZIM3-FLAG-SpdCas9-2xNLS-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A/H840A
    Promoter
    eIFa
    Available since
    Oct. 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    248901-plasmid-map-sequence-id-498950

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  15. pAAV2-UbC-3xNLS-mScarlet-I

    Plasmid
    #191099
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Publication
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-mScarlet-I
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    optimized to human codon usage
    Promoter
    UbC
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191099-plasmid-map-sequence-id-380113

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Showing: 4161 - 4180 of 18824 results