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We narrowed to 1,557 results for: Flp

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Showing: 801 - 840 of 1557 results

  2. pTO_CHMP2B_LAP

    Plasmid
    #101848
    Purpose
    Tet-inducible expression of human CHMP2B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    CHMP2B (CHMP2B Human)
    Use
    Flp-frt recombination, tet-inducible expression
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Mutation
    silent mutation in Thr 104; A to T
    Promoter
    CMV
    Available Since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    101848-plasmid-map-sequence-id-200235

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  14. pcDNA5/FRT/TO/Flag-tGFP

    Plasmid
    #110375
    Purpose
    Mammalian expression of turboGFP with FLAG tag, Flp-In T-Rex system
    Depositor
    Sandra Martha Gomes Dias
    Insert
    turboGFP
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110375-plasmid-map-sequence-id-213401

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  15. mT2A-tdTomato-FNF

    Plasmid
    #182340
    Purpose
    Recombineering cassette plasmid for T2A-tdTomato-FNF
    Depositor
    Mario Capecchi
    Insert
    T2A-tdTomato-FNF
    Use
    Mouse Targeting; Flp / frt
    Expression
    Mammalian
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182340-plasmid-map-sequence-id-358215

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  16. pMOD4-mT2A-mutated_rpsL-BSD-F

    Plasmid
    #182338
    Purpose
    Template plasmid for PCR amplification of initial recombineering cassette
    Depositor
    Mario Capecchi
    Insert
    BSD
    Use
    Mouse Targeting; Flp / frt
    Expression
    Bacterial
    Available Since
    April 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    182338-plasmid-map-sequence-id-358191

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  17. F3 Neo* F3

    Plasmid
    #140235
    Purpose
    F3 (FRT variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
    Depositor
    Mario Capecchi
    Insert
    Neo*
    Use
    Mouse Targeting; Flp/ frt
    Expression
    Bacterial and Mammalian
    Available Since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140235-plasmid-map-sequence-id-316186

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  18. mT2A-td-sfGFP-FNF

    Plasmid
    #182339
    Purpose
    Recombineering cassette plasmid for T2A-td-sfGFP-FNF
    Depositor
    Mario Capecchi
    Insert
    T2A-td-sfGFP-FNF
    Use
    Mouse Targeting; Flp / frt
    Expression
    Mammalian
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182339-plasmid-map-sequence-id-358195

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  19. pRDA_852

    Plasmid
    #185527
    Purpose
    Lentiviral vector that enables Cre-mediated expression of two sgRNAs. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR, Cre/Lox, and Lentiviral ; Flp/frt
    Expression
    Mammalian
    Promoter
    Human U6 and Mouse U6
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    185527-plasmid-map-sequence-id-366261

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  20. pTO_CHMP4B_LAP

    Plasmid
    #101850
    Purpose
    Tet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    Charged Multivesicular Body Protein 4B (CHMP4B Human)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    101850-plasmid-map-sequence-id-200248

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  21. pAAV-nEF-Con/Fon DREADD Gi-mCherry

    Plasmid
    #177672
    Purpose
    Expresses DREADD hM4D (Gi) in Cre and Flp expressing cells
    Depositor
    Karl Deisseroth
    Has Service
    AAV8
    Insert
    DREADD Gi-mCherry
    Use
    AAV, Cre/Lox, and Mouse Targeting ; Intrsect
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    nEF
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177672-plasmid-map-sequence-id-348533

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  22. pAAV-nEF-Con/Foff DREADD Gi-mCherry

    Plasmid
    #177673
    Purpose
    Expresses DREADD hM4D (Gi) only in Cre expressing cells and not in Flp expressing cells
    Depositor
    Karl Deisseroth
    Has Service
    AAV8
    Insert
    DREADD Gi-mCherry
    Use
    AAV, Cre/Lox, and Mouse Targeting ; Intrsect
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    nEF
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177673-plasmid-map-sequence-id-348532

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  23. H2B-sfGFP-T2A-iCre

    Plasmid
    #178546
    Purpose
    pCMV-Histone H2B-sfGFP-T2A-iCre-bpA-FRT-Neo*-FRT
    Depositor
    Mario Capecchi
    Insert
    Histone H2B-sfGFP-T2A-iCre
    Use
    Cre/Lox and Mouse Targeting; Flp/frt
    Expression
    Mammalian
    Available Since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    178546-plasmid-map-sequence-id-360157

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  26. pSAH66 - [BAGp | gfp | tbb-2 UTR]

    Plasmid
    #200340
    Purpose
    gfp BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.
    Depositor
    Christian Froekjaer-Jensen
    Insert
    [BAGp | gfp | tbb-2 UTR]
    Expression
    Worm
    Promoter
    WBGene00001536, flp-17
    Available Since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    200340-plasmid-map-sequence-id-418120

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  27. pSAH65 - [BAGp | wrmScarlet | tbb-2 UTR]

    Plasmid
    #200339
    Purpose
    mScarlet BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.
    Depositor
    Christian Froekjaer-Jensen
    Insert
    [BAGp | wrmScarlet | tbb-2 UTR]
    Expression
    Worm
    Promoter
    WBGene00001536, flp-17
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    200339-plasmid-map-sequence-id-409767

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  28. tRECs/3xUAS-FP attB

    Plasmid
    #220330
    Purpose
    encodes three Fluorescent Protein FLP-OUT cassettes responsive to three different recombinases (mFLP5, B3 and KD)
    Depositor
    Erdem Bangi
    Inserts
    B3RT-STOP-B3RT-TagBFP-3xHA
    KDRT-STOP-KDRT-GFP-HSV
    FRTm71-STOP-FRTm71-mCherry
    Expression
    Insect
    Promoter
    UAS
    Available Since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    220330-plasmid-map-sequence-id-441802

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  29. pAAV-hSyn-frt-DIO-hM3D(Gq)-mCherry

    Plasmid
    #217397
    Purpose
    Expresses the hM3D(Gq) excitatory DREADD fused to mCherry, in a Cre-ON and FLP-OFF dependent manner, under the control of hSyn promoter
    Depositor
    Oscar Marin
    Insert
    hM3D(Gq)-mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    human Synapsin 1
    Available Since
    May 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217397-plasmid-map-sequence-id-433248

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  30. pAAV-hSyn-frt-DIO-hM4D(Gi)-mCherry

    Plasmid
    #217398
    Purpose
    Expresses the hM4D(Gi) inhibitory DREADD fused to mCherry, in a Cre-ON and FLP-OFF dependent manner, under the control of hSyn promoter
    Depositor
    Oscar Marin
    Insert
    hM4D(Gi)-mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    human Synapsin 1
    Available Since
    May 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217398-plasmid-map-sequence-id-433249

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  32. mT2A-sfGFP-linker-iCre-ERT2-FNF

    Plasmid
    #182341
    Purpose
    Recombineering cassette plasmid for T2A-sfGFP-linker-iCre-ERT2-FNF
    Depositor
    Mario Capecchi
    Insert
    T2A-sfGFP-linker-iCre-ERT2-FNF
    Use
    Mouse Targeting; Flp / frt
    Expression
    Mammalian
    Available Since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    182341-plasmid-map-sequence-id-359742

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  38. pcDNA5/FRT/TO/Flag-tGFP-Cryz

    Plasmid
    #110378
    Purpose
    Mammalian expression of turboGFP-CRYZ with FLAG tag, Flp-In T-Rex system
    Depositor
    Sandra Martha Gomes Dias
    Insert
    crystallin zeta (CRYZ Human)
    Tags
    FLAG and tGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110378-plasmid-map-sequence-id-213403

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Showing: 801 - 840 of 1557 results