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Plasmid#72937PurposeLevel 1 Destination vector - Position CDepositorInsertLevel 1 - C site
UseSynthetic BiologyTagsExpressionMutationBeta-lactamase - G717C (nucleotide) - silent muta…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU1-D-lacZ
Plasmid#72938PurposeLevel 1 Destination vector - Position DDepositorInsertLevel 1 - D site
UseSynthetic BiologyTagsExpressionMutationBeta-lactamase - G717C (nucleotide) - silent muta…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU1-C-RFP
Plasmid#72941PurposeLevel 1 Destination vector - Position CDepositorInsertLevel 1 - C site
UseSynthetic BiologyTagsExpressionMutationBeta-lactamase - G717C (nucleotide) - silent muta…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU1-D-RFP
Plasmid#72942PurposeLevel 1 Destination vector - Position DDepositorInsertLevel 1 - D site
UseSynthetic BiologyTagsExpressionMutationBeta-lactamase - G717C (nucleotide) - silent muta…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU1-D1-RFP
Plasmid#72943PurposeLevel 1 Destination vector - Position D1DepositorInsertLevel 1 - D1 site
UseSynthetic BiologyTagsExpressionMutationBeta-lactamase - G717C (nucleotide) - silent muta…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU1-E-RFP
Plasmid#72944PurposeLevel 1 Destination vector - Position EDepositorInsertLevel 1 - E site
UseSynthetic BiologyTagsExpressionMutationBeta-lactamase - G717C (nucleotide) - silent muta…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-ORF-eGFP
Plasmid#72960PurposeLevel 0 Entry vector" ORF - eGFP "DepositorInsertEnhanced green flourescent protein (eGFP)
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 RRN3
Plasmid#67112PurposeStandard for cell-free expression benchmarking.DepositorInsertRRN3 (RRN3 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TFAP4
Plasmid#67073PurposeStandard for cell-free expression benchmarking.DepositorInsertTFAP4 (TFAP4 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationP338APromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ki67-mCherry
Plasmid#183748PurposeExpresses EGFP-Ki67-mCherryDepositorInsertMKI67 (MKI67 Human)
UseTagsKi-67 with N-terminal EGFP tag and Ki-67 with C-…ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
EpsilonCOP EGFP
Plasmid#128151PurposeExpression of epsilon COPDepositorInsertepsilon COP (COPE Bovine)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ki67∆Nterm-mNeonGreen-IRESpuro2
Plasmid#183740PurposeExpresses Ki67∆Nterm-mNeonGreenDepositorInsertMKI67 LR domain + all Ki-67 repeats (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ki67∆FHA-mNeonGreen-IRESpuro2
Plasmid#183739PurposeExpresses Ki67∆FHA-mNeonGreenDepositorInsertMKI67_lacking FHA (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationMKI67(lacking FHA)PromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2
Plasmid#183741PurposeExpresses Ki67 (8 repeats+LR)-mNeonGreenDepositorInsertMKI67 LR domain + 8 Ki-67 repeats (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationMKI67 LR domain + 8 Ki-67 repeatsPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMP11
Plasmid#215559PurposepKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 originDepositorInsertsCas9
gRNA targeting pBR322 origin of replication
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23105 and tetAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CYP3A4
Plasmid#67104PurposeStandard for cell-free expression benchmarking.DepositorInsertCYP3A4 (CYP3A4 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CYP2D6
Plasmid#67090PurposeStandard for cell-free expression benchmarking.DepositorInsertCYP2D6 (CYP2D6 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pDECKO_mCherry
Plasmid#78534PurposeBackbone to clone single or paired sgRNAsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterU6 (for expressing sgRNA)Available sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
EGFP-mCherry-Ki67
Plasmid#183750PurposeExpresses EGFP-mCherry-Ki67DepositorInsertMKI67 (MKI67 Human)
UseTagsEGFP and mCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-GST
Plasmid#171486PurposeT7 promotor drives in vitro transcription of GST mRNADepositorInsertGST
UsePgehmeTagsExpressionMutationPromoterT7Available sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFAP-Cre
Plasmid#24704DepositorInsertCre
UseTagsExpressionMammalianMutationPromoterGFAP (glial fibrillary acidic protein)Available sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
Ki67 (LR)-mNeonGreen-IRESpuro2
Plasmid#183743PurposeExpresses Ki67 (LR)-mNeonGreenDepositorInsertMKI67 LR domain (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationLR domain of Ki67PromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBP-J23100
Plasmid#72963PurposeLevel 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)DepositorInsertJ23100 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ptrc99a-pVhb-tdTER-fdh
Plasmid#215649PurposepBR322 Origin, AmpR, with tdTER and Fdh genes under Vhb promoterDepositorInsertstrans-enoyl-CoA reductase
formate dehydrogenase
UseTagsExpressionBacterialMutationPromoterVhbAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 WAVE 2
Plasmid#67098PurposeStandard for cell-free expression benchmarking.DepositorInsertWAVE 2 (WASF2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBTRCk-pVhb-maACR
Plasmid#215651PurposepBBR1 Origin, KanR, with maACR gene under Vhb promoterDepositorInsertacyl-CoA reductase
UseTagsMaltose binding protein and His tagExpressionBacterialMutationPromoterVhbAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-pVhb-vfFadBA
Plasmid#215650PurposepACYC Origin, CmR, with vfFadBA gene under Vhb promoterDepositorInsertthiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
UseTagsExpressionBacterialMutationPromoterVhbAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ki67∆repeats-mNeonGreen-IRESpuro2
Plasmid#183742PurposeExpresses Ki67∆repeats-mNeonGreenDepositorInsertMKI67(lacking repeats) (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationMKI67(lacking repeats)PromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a - SodA
Plasmid#89612PurposeExpresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG inductionDepositorInsertSuperoxide dismutase A
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLMB757
Plasmid#83792PurposeFructose biosensor plasmidDepositorInsertRegion upstream RL0489 (frcB) containing all of RL0488 (frcR)
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a - APX
Plasmid#89618PurposeExpresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertAscorbate dependent Tryparedoxin peroxidase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLMB713
Plasmid#83785PurposeSalicylic acid biosensor plasmidDepositorInsertRegion upstream RL1329 (salA) containing all of RL1328 (salR)
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 RUVBL1
Plasmid#67091PurposeStandard for cell-free expression benchmarking.DepositorInsertRUVBL1 (RUVBL1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 IL2
Plasmid#67053PurposeStandard for cell-free expression benchmarking.DepositorInsertIL2 (IL2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mClover3
Plasmid#171497PurposeT7 promotor drives in vitro transcription of mClover3 mRNADepositorInsertmClover3
UsePgehmeTagsExpressionMutationPromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PDGFRB
Plasmid#67130PurposeStandard for cell-free expression benchmarking.DepositorInsertPDGFRB (PDGFRB Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
H2B-2A-GFP-IRESpuro2
Plasmid#183746PurposeExpresses H2B-2A-GFPDepositorInsertH2B (H2BC16P Human)
UseTagsGFP with 2A protease cleavage site between H2B an…ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a - CPX
Plasmid#89620Purpose[Edit] Expresses recombinant 6HIS tagged T. cruzi cytoplasmic tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertCytosolic Tryparedoxin peroxidase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLMB577
Plasmid#83778PurposePhenylalanine biosensor plasmidDepositorInsertpromoter of RL1860
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only