Skip to main content
Addgene
Showing: 401 - 420 of 12605 results

  1. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG E1322R

    Plasmid
    #166814
    Purpose
    Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity
    Depositor
    Hao Wu
    Article
    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586
    Insert
    NLRP1 (NLRP1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    E1322R
    Promoter
    CMV
    Available since
    May 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    166814-plasmid-map-sequence-id-331962

    Enlarge

  2. pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE P1214R K1277E

    Plasmid
    #166811
    Purpose
    Mammalian cell expression vector for FLAG-tagged NLRP1. LL1193EE and P1214R mutations abolish DPP9 association, K1277E abolishes UPA-UPA contacts
    Depositor
    Hao Wu
    Article
    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586
    Insert
    NLRP1 (NLRP1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    LL1193EE, P1214R, K1277E
    Promoter
    CMV
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166811-plasmid-map-sequence-id-328312

    Enlarge

  3. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG H1276G

    Plasmid
    #166812
    Purpose
    Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity
    Depositor
    Hao Wu
    Article
    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586
    Insert
    NLRP1 (NLRP1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    H1276G
    Promoter
    CMV
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166812-plasmid-map-sequence-id-328391

    Enlarge

  4. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG K1277E

    Plasmid
    #166813
    Purpose
    Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity
    Depositor
    Hao Wu
    Article
    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586
    Insert
    NLRP1 (NLRP1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    K1277E
    Promoter
    CMV
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166813-plasmid-map-sequence-id-328384

    Enlarge

  15. Anti-Slo2.1/Slick K+ channel [N11/33R-rat IgG2a] - Chimeric

    Plasmid
    #231829
    Purpose
    Mammalian expression plasmid of anti-Slo2.1/Slick K+ channel (Mouse) rat IgG2a R-mab. Derived from hybridoma N11/33.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-Slo2.1/Slick K+ channel (Mus musculus) recombinant mouse monoclonal antibody (Kcnt2 Chimera: M. musculus (mouse)/R. norvegicus (rat))
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    231829-plasmid-map-sequence-id-459956

    Enlarge

  16. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T4DNApol (JO638)

    Plasmid
    #208956
    Purpose
    A variant CE1 construct with T4 DNA polymerase (-exo, activity enhanced), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-T4pol-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A), T4Pol(-exo;D189A/E191A/L412M)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208956-plasmid-map-sequence-id-415745

    Enlarge

  17. HUHe variant click editor (CE1) - pCMV-T7-DCV-nCas9-EcKlenow (JO608)

    Plasmid
    #208946
    Purpose
    A variant CE1 construct with DCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    DCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208946-plasmid-map-sequence-id-415680

    Enlarge

  18. HUHe variant click editor (CE1) - pCMV-T7-MSMV-nCas9-EcKlenow (JO661)

    Plasmid
    #208947
    Purpose
    A variant CE1 construct with MSMV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    MSMV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208947-plasmid-map-sequence-id-415686

    Enlarge

  19. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29 (LM2993)

    Plasmid
    #208959
    Purpose
    A variant CE1 construct with ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); ePhi29(-exo)(D169A;M8R/V51A/M97T/…
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208959-plasmid-map-sequence-id-415785

    Enlarge

Showing: 401 - 420 of 12605 results