We narrowed to 10,138 results for: SEC
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Plasmid#175453PurposeProtein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649.DepositorInsertCD44 (CD44 Human)
UseTagsHis6ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
Drosophila_sgRNA_group_3
Pooled Library#134584PurposeTargets all other Drosophila genes not included in Groups 1 and 2DepositorExpressionInsectSpeciesDrosophila melanogasterUseCRISPRAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Drosophila_sgRNA_group_1
Pooled Library#134582PurposeTargets kinases, phosphatases, and Drosophila orthologs of drug targetsDepositorExpressionInsectSpeciesDrosophila melanogasterUseCRISPRAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Drosophila_sgRNA_group_2
Pooled Library#134583PurposeTargets Drosophila orthologs of vertebrate genes that have undergone expansion in the vertebrate lineDepositorExpressionInsectSpeciesDrosophila melanogasterUseCRISPRAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
MoClo Pichia toolkit
Plasmid Kit#1000000108PurposeA collection of control elements and fluorescent reporters that can be used in combination with the MoClo-YTK toolkit for protein expression and secretion in yeast (Pichia pastoris).DepositorAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETconNK-TEM1(S70A,D179G)
Plasmid#81166PurposepETconNK with TEM1(S70A,D179G) beta-lactamaseDepositorInsertTEM-1 beta-lactamase
UseTagsc-mycExpressionBacterial and YeastMutationDeleted the sec transport tag. Plasmid encodes H2…PromoterGAL1 and gal1Available sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSO7
Plasmid#106365PurposeTo insert a single copy of a construct at a site on Chr I, corresponding to the Shigella flexneri protein OspF (a MAP kinase inhibitor) expressed specifically in the epidermis.DepositorInsertttTi4348_ SEC _pcol-19_OspF_3'unc54UTR
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHW1133
Plasmid#229869PurposeDestination RMCE vector containing FRT::SA::51-bp Artificial exon::3xStop::SL2::SapI Insertion sites (to clone new driver with 3'UTR in slot 5 and SEC in slot 6)::reverse FRT3 to swap driver via RMCEDepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMS110
Plasmid#215674PurposeSplit hygromycinR landing pad insertion plasmid for ChrIIIDepositorInsert5'HA + synthetic guide site3 + 3'ΔHygR:unc-54 3' UTR + lox2272 + SEC + lox2272 + 3'HA
UseCRISPR and Cre/LoxTagsExpressionWormMutation3' ∆HYGR is promoterless and encodes aa60-341PromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSO9
Plasmid#106366PurposeTo insert a single copy of a construct at a site on Chr I, corresponding to the Shigella flexneri protein OspF (a MAP kinase inhibitor) expressed specifically in the intestine.DepositorInsertttTi4348_ SEC _pvit-6_OspF_3'unc54
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
mU6-CR1-CRISPRiv2_hU6-CR3-NT-ctrl-guide_EF1a-PuroR-T2A-BFP
Pooled Library#197348PurposeTo allow for genome-wide genetic modifier screening, the library has a second fixed non-targeting control guide.DepositorSpeciesHomo sapiensUseLentiviralAvailable sinceMay 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
mU6-CR1-CRISPRiv2_hU6-CR3-EMC2-guide_EF1a-PuroR-T2A-BFP
Pooled Library#197349PurposeTo allow for genome-wide genetic modifier screening, the library has a second fixed guide targeting EMC2.DepositorSpeciesHomo sapiensUseLentiviralAvailable sinceMay 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
Oxford Drosophila melanogaster Genome-wide CRISPR Library
Pooled Library#64750PurposeA genome-wide CRISPR library covering 13,501 genes (98.8% of the Drosophila melanogaster genome), among which 8,989 genes are targeted by three or more independent single guide RNAs (sgRNAs).DepositorExpressionInsectSpeciesDrosophila melanogaster, Streptococcus pyogenes, and SyntheticUseCRISPRAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSALECTNK-TEM1(S70A,D179G)/csTEM1
Plasmid#81163PurposepSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1DepositorInsertTEM-1 beta-lactamase
UseTagsCodon swapped TEM-1 from H26 to W290 and His6xExpressionBacterialMutationDeleted the sec transport tag. Plasmid encodes H2…Promoterlac promoterAvailable sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
NDV-HN-SpyTag
Plasmid#160000PurposeExpresses Newcastle disease virus HN protein (47-575) with C-terminal SpyTag fusion in mammalian cellsDepositorInsertNDV-HN-SpyTag
UseTagsHis6-tagExpressionMammalianMutationPromoterChicken beta-actin promoterAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTT610
Plasmid#101742Purposeencoding SecY(L2V, R252G)–(GGSG)4–SecA1-939–His10 fusion protein and SecE and SecG from Thermus thermophilus HB8DepositorUseTagsHis10ExpressionBacterialMutationSecY(L2V, R252G)–(GGSG)4–SecA1-939–His10 fusion p…PromoterLacAvailable sinceNov. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAK22
Plasmid#101741Purposeencoding two copies of T. thermophilus SecG for expression of SecYEG complexDepositorInsertThermus thermophilus HB8 SecG x 2
UseTagsNONEExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
E. coli K-12 MG1655_rLP5(nth-ydgR)
Bacterial Strain#110245PurposeE. coli K-12 MG1655 with Landing Pad engineered into the nth-ydgR intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.DepositorBacterial ResistanceChloramphenicolAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
E. coli K-12 MG1655_fLP4(essQ-cspB)
Bacterial Strain#110244PurposeE. coli K-12 MG1655 with Landing Pad engineered into the essQ-cspB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.DepositorBacterial ResistanceChloramphenicolAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
E. coli K-12 MG1655_rLP6(ygcE-ygcF)
Bacterial Strain#110246PurposeE. coli K-12 MG1655 with Landing Pad engineered into the ygcE-ygcF intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.DepositorBacterial ResistanceChloramphenicolAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only