We narrowed to 6,522 results for: poly

1. C417-E01: attB4-polyhedrinp>His6-nostop-attB1r

   Plasmid

   #162932

   Purpose

   Gateway attB4/attB1r entry clone for baculovirus expression of His6 tagged proteins using the polyhedrin promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6 fusion with AcMNPV polyhedrin promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-SOUL-P2A-TdTomato-WPRE-hGH-polyA

   Plasmid

   #177576

   Purpose

   Expresses SOUL in neuronal cell types

   Depositor

   Guoping Feng

   Article

   Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1

   Insert

   SOUL
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ9824: CMV-SV40_NLS-CasMINI-V3.1--3xFlag- c-Myc_NLS-polyA

   Plasmid

   #176271

   Purpose

   Expression of CasMINI-V3.1 in mammalian cells.

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI-V3.1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato

   Plasmid

   #138610

   Purpose

   Express TRACE editor together with a tdTomato reporter in pcDNA3.1(+) backbone

   Depositor

   Fei Chen

   Article

   Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Inserts

   Hyperactive AID
   T7 RNA Polymerase
   tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac cloning vector with BioBrick PolyPromoter LIC Subcloning (438-A)

   Plasmid

   #55218

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-TgKI-MCS-p2A-Venus-PEST-MCS-polyA

   Plasmid

   #174030

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   p2A-Venus-PEST
   

   Use

   Zebrafish knock-in tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA

   Plasmid

   #107311

   Purpose

   Encoding scFv-p65-HSF1 driven by EF1α promoter

   Depositor

   Hui Yang

   Article

   Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

   Insert

   EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Polymerase-unfused click editor - pCMV-T7-PCV2-nCas9 (LM2702)

   Plasmid

   #208961

   Purpose

   Polymerase-unfused click editor comprised of PCV2 fused to nCas9, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET E. coli expression vector with BioBrick polypromoter restriction sites (14-A)

   Plasmid

   #48307

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA

    Plasmid

    #107313

    Purpose

    Encoding aCamkII-mCherry-Cre

    Depositor

    Hui Yang

    Article

    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

    Insert

    aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET LIC cloning vector with BioBrick polycistronic restriction sites (9A)

    Plasmid

    #48283

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSF4 CMV 5TOP intron renilla TRICK CTE polyA

    Plasmid

    #64544

    Purpose

    5′ TOP TRICK reporter mRNA

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    12x PP7 stem-loops
    

    Use

    Tags

    24xMS2 stem loops, 5' TOP (5′ terminal oligo…

    Expression

    Mammalian

    Mutation

    Promoter

    Tetracycline-inducible promoter and 5′ UTR of hum…

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. Polymerase-recruited click editor - pCMV-T7-PCV2-nCas9-CC(N6) (LM2705)

    Plasmid

    #208962

    Purpose

    Polymerase-recruited click editor comprised of PCV2 fused to nCas9 with a C-terminal N6 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-CC(N6)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. C513-E08: attB5r-<p10p-polyhedrinp>H6-MBP-nostop-attB1r

    Plasmid

    #162950

    Purpose

    Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors; allows His6-MBP fusion for the polyhedrin transcribed protein

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin) with His6-maltose binding protein fusion tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. C417-E03: attB4-polyhedrinp>His6-GST-nostop-attB1r

    Plasmid

    #162934

    Purpose

    Gateway attB4/attB1r entry clone for baculovirus expression of His6-GST tagged proteins using the polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-glutathione-S-transferase fusion, AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. C417-E02: attB4-polyhedrinp>His6-MBP-nostop-attB1r

    Plasmid

    #162933

    Purpose

    Gateway attB4/attB1r entry clone for baculovirus expression of His6-MBP tagged proteins using the polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-maltose binding protein fusion, AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGP-CAG-Ace2N-4AA-mNeon A122D-WPRE-bGH-polyA

    Plasmid

    #172911

    Purpose

    Mammalian expression of voltage sensor

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Insert

    Ace2N-4AA-mNeon A122D
    

    Use

    Tags

    soma localization targeting sequence

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    CAG

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

    Plasmid

    #166693

    Purpose

    Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.

    Depositor

    Elvir Becirovic

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Inserts

    dCas9-VPR
    3x Cnga1 promoter-targeting sgRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and U6

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS-BsmbI-LoxP-Ef1a-EM7-mCherry-Neo-PolyA-LoxP

    Plasmid

    #192822

    Purpose

    Golden Gate compatible plasmid with mCherry and Neo

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    mCherry and Neo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFastBac LIC cloning vector with BioBrick polycistronic restriction sites (11A)

    Plasmid

    #48294

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge