We narrowed to 1,255 results for: post

1. pHPHS927

   Plasmid

   #228238

   Purpose

   Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible β-globin reporter for integration into Bxb1 attP_GA landing pad

   Depositor

   Arvind Rasi Subramaniam

   Article

   Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

   Inserts

   mCherry
   Beta globin
   

   Use

   Tags

   3xFLAG, HA, and PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GV and none

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Dyn1 R271E

   Plasmid

   #198343

   Purpose

   Mammalian expression plasmid of GFP-chimera proteins.

   Depositor

   Justin Taraska

   Article

   Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

   Insert

   Dynamin1 (DNM1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R271E

   Promoter

   CMV

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Dyn1 K44A

   Plasmid

   #198340

   Purpose

   Mammalian expression plasmid of GFP-chimera proteins.

   Depositor

   Justin Taraska

   Article

   Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

   Insert

   Dynamin1 (DNM1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K44A

   Promoter

   CMV

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Dyn1 D406A

   Plasmid

   #198341

   Purpose

   Mammalian expression plasmid of GFP-chimera proteins.

   Depositor

   Justin Taraska

   Article

   Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

   Insert

   Dynamin1 (DNM1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D406A

   Promoter

   CMV

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-N3-hARL1(Q71L)

   Plasmid

   #206407

   Purpose

   Mammalian expression of hARL1(Q71L)

   Depositor

   Kazuhisa Nakayama, Hye-Won Shin

   Article

   Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

   Insert

   ARL1 (ARL1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q71L

   Promoter

   Available Since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-N3-hARL1(T31N)

   Plasmid

   #206408

   Purpose

   Mammalian expression of hARL1(T31N)

   Depositor

   Kazuhisa Nakayama, Hye-Won Shin

   Article

   Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

   Insert

   ARL1 (ARL1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T31N

   Promoter

   Available Since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. GAL-ARF

   Plasmid

   #89121

   Purpose

   mammalian expression of p14-hARF linked to GAL4 DNA binding domain

   Depositor

   Elizabeth Wilson

   Article

   Minges et al J Biol Chem. 2015 Oct 9;290(41):25174-87

   Insert

   p14-hARF (CDKN2A Human)
   

   Use

   Tags

   GAL4 DBD

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS424-TUB2 - human TUBB3 Cterminal tail delta K

   Plasmid

   #60404

   Purpose

   Expression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) without last 4 amino acids--QGPK

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   TUB2 amino acids 429 - end, replaced with human T…

   Promoter

   GAL

   Available Since

   May 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pHPHS232

   Plasmid

   #228356

   Purpose

   Landing pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locus

   Depositor

   Arvind Rasi Subramaniam

   Article

   Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

   Inserts

   mTagBFP2
   Reverse tetracycline transactivator 3
   neo
   

   Use

   Tags

   Blasticidin S deaminase and iCasp9

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GV and gene trap

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. mEosEM-E_Lamin A

    Plasmid

    #226338

    Purpose

    Photoswitchable fluorescent protein for correlative light-electron microscopy; nuclear envelope-targeted mEosEM-E for mammalian expression

    Depositor

    Pingyong Xu

    Article

    Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

    Insert

    Lamin A (LMNA Human)
    

    Use

    Tags

    mEosEM-E

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGART-2XStrep

    Plasmid

    #125681

    Purpose

    expresses human GART protein with a C-terminal 2XStrep affinity tag

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

    Insert

    GART (GART Human)
    

    Use

    Tags

    2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Mito-mEosEM-E

    Plasmid

    #226335

    Purpose

    Photoswitchable fluorescent protein for correlative light-electron microscopy; mitochondria-targeted mEosEM-E for mammalian expression

    Depositor

    Pingyong Xu

    Article

    Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

    Insert

    COX8A (COX8A Human)
    

    Use

    Tags

    mEosEM-E

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-NOS-IN133.3xFLAG.mCherry-WPRE

    Plasmid

    #127868

    Purpose

    pAAV plasmid expressing an NOS-IN133.3xFLAG.mCherry fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    Nos1 (Nos1 Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Expression

    Mutation

    Amino acids 1-133 only

    Promoter

    hSyn

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. HisGB1-Hif1a(776-826) + CBP TAZ1(340-439)

    Plasmid

    #99343

    Purpose

    Bacterial expression of mouse CBP TAZ1 domain as coexpression with Hif1a TAD

    Depositor

    Peter Wright

    Article

    Sugase et al Protein Expr Purif. 2008 Feb;57(2):108-1

    Inserts

    Hif1a (776-826) (HIF1A Human)
    CBP (340-439)
    

    Use

    Tags

    His6GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA HA-Gnat1 WT-3UTR

    Plasmid

    #184026

    Purpose

    Expresses mouse Gnat1 protein with N-terminal HA tag from cDNA that contains the full length 3'-UTR

    Depositor

    Peter Stoilov

    Article

    Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

    Insert

    Gnat1 (Gnat1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MAP4K3-GFP K45E

    Plasmid

    #21734

    Depositor

    L. Miguel Martins

    Article

    Lam et al Proc Natl Acad Sci U S A. 2009 Jul 21;10

    Insert

    MAP4K3 (MAP4K3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Mutation of the ATP-binding site (K45E). This ver…

    Promoter

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pPPAT-11AA-2XStrep

    Plasmid

    #125680

    Purpose

    expresses human PPAT protein, with first 11 residues removed, with a C-terminal 2XStrep affinity tag

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

    Insert

    PPAT (PPAT Human)
    

    Use

    Tags

    2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    the first eleven residues have been removed

    Promoter

    CMV

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-p18Ink4c-IRES-GFP

    Plasmid

    #52220

    Purpose

    retroviral bicistronic expression of p18Ink4c and GFP

    Depositor

    Martine Roussel

    Article

    Forget et al Cell Cycle. 2008 Dec;7(23):3737-46. Epub

    Insert

    Ink4c (Cdkn2c Mouse)
    

    Use

    Retroviral

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pGLB2 5′ mouse CNS

    Plasmid

    #49348

    Purpose

    luciferase reporter with mouse Bmp2 promoter and 3' conserved noncoding sequence (CNS)

    Depositor

    Melissa Rogers

    Article

    Fritz et al J Biol Chem. 2004 Nov 19;279(47):48950-8

    Inserts

    Bmp2 distal promoter (Bmp2 Mouse)
    Bmp2 3' CNS (conserved noncoding sequence) (Bmp2 Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    -

    Available Since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pADSL-2XStrep

    Plasmid

    #125684

    Purpose

    expresses human ADSL protein with a C-terminal 2XStrep affinity tag

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

    Insert

    ADSL (ADSL Human)
    

    Use

    Tags

    2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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