We narrowed to 22,489 results for: SCR

1. pLV-tet2puro-gal4DBD-HIFCAD

   Plasmid

   #207173

   Purpose

   Dox Inducible Expression Plasmid for gal4DBD-HIF-CAD

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Insert

   Gal4DBD-HIF1a-CAD
   

   Use

   Lentiviral; Dox inducible

   Tags

   2x Myc

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. eSpCas9-EF

   Plasmid

   #177267

   Purpose

   CRISPR vector

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPPC016.109

   Plasmid

   #171144

   Purpose

   Expression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-GmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   J109 scRNA
   J1-BBa_J23117-mRFP
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119 and J1-BBa_J23117

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPPC008.106

   Plasmid

   #171141

   Purpose

   Expression of J106 scRNA on pBBR1-GmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   J106 scRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSTAP-seq_fly_spike-in

   Plasmid

   #125151

   Purpose

   Vector to measure CP candidates (spike-in) expression by determining the abundance of transcripts originating from the candidate by a D.pse enhancer in fly cells

   Depositor

   Alexander Stark

   Article

   Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

   Insert

   D. pseudoobscura peak #47 enhancer
   

   Use

   Stap-seq screening vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-REPORT(PGK/CMV)-12xHRE

   Plasmid

   #172335

   Purpose

   hypoxic responsive reporter

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   d2eGFP
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3x SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   PGK/CMV

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiFUCCI(CA)5

   Plasmid

   #223176

   Purpose

   Lentiviral fluorescent ubiquitination-based cell cycle indicator (FUCCI)

   Depositor

   Neville Sanjana

   Article

   Liang et al Cell. 2024 Dec 26;187(26):7637-7654.e29.

   Insert

   FUCCI(CA)5
   

   Use

   Lentiviral

   Tags

   AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiGuide_DKO

   Plasmid

   #183193

   Purpose

   Plasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

   Depositor

   Lang Li

   Article

   Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, mU6

   Available since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMax_GFP

   Plasmid

   #177825

   Purpose

   Host Cell Reactivation (HCR) control GFP plasmid (pMax backbone)

   Depositor

   Zachary Nagel, Leona Samson

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMax_mOrange

    Plasmid

    #177827

    Purpose

    Host Cell Reactivation (HCR) control mOrange plasmid (pMax backbone)

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    mOrange
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-ATR Kinase dead

    Plasmid

    #31612

    Depositor

    Aziz Sancar

    Article

    Jiang et al Mol Cell Biol. 2006 Jan . 26(1):39-49.

    Insert

    ATR (ATR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K2327R (kinase dead)

    Promoter

    Available since

    Nov. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB533-44B12m23-sfGFP

    Plasmid

    #186778

    Purpose

    RNAP2 Ser5ph-mintbody expression vector for mammalian cells

    Depositor

    Hiroshi Kimura

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    44B12m23-scFv
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLR5-CAG-NLS-SNAP

    Plasmid

    #174879

    Purpose

    PiggyBac transposon vector for expression of NLS-SNAP

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    NLS-SNAP
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. UAS:mCherry

    Plasmid

    #170994

    Purpose

    UAS:GAL4 repeat-containing promoter drives simple mCherry reporter gene. Blasticidin selectable.

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    UAS:mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synthetic 14x UAS:Gal4

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPPC019.AAV

    Plasmid

    #171147

    Purpose

    Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-KmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    hAAVS1 scRNA
    J1-BBa_J23117-mRFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 and J1-BBa_J23117

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. dSpCas9-VPR

    Plasmid

    #205247

    Purpose

    In vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.

    Depositor

    Rasmus Bak

    Article

    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

    Insert

    dSpCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-SFFVp-CRISPRoffv2.1-IRES-BlastR

    Plasmid

    #207181

    Purpose

    Lentiviral Expression Plasmid for CRISPRoff2.1 driven by SFFV Promoter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Insert

    CRISPRoffv2.1
    

    Use

    CRISPR and Lentiviral

    Tags

    HA and TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFVp

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. CROP-sgRNA-MS2

    Plasmid

    #153457

    Purpose

    CROP-seq vector with mCherry and x2 MS2 loops in gRNA scaffold

    Depositor

    Wolf Reik

    Article

    Alda-Catalinas et al Cell Syst. 2020 Jul 22;11(1):25-41.e9. d

    Insert

    sgRNA empty backbone
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. mAIRN HO

    Plasmid

    #207411

    Purpose

    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    mAIRN
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. mAIRN CD

    Plasmid

    #207412

    Purpose

    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    mAIRN
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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