We narrowed to 18,031 results for: Mut

1. pCBLsgRNA2

   Plasmid

   #199728

   Purpose

   Golden Gate entry vector; 2nd gRNA under OsU3 promoter

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   OsU3-sgRNA2 scaffold
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. prNFL-EGFP

   Plasmid

   #132605

   Purpose

   Expresses EGFP-tagged rat NFL

   Depositor

   Anthony Brown

   Article

   Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

   Insert

   Nefl (Nefl Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEYFP-N1-HsSmg5_H

   Plasmid

   #146474

   Purpose

   Mammalian Expression of HsSmg5

   Depositor

   Elisa Izaurralde

   Article

   Unterholzner et al Mol Cell. 2004 Nov 19. 16(4):587-96.

   Insert

   HsSmg5 (SMG5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   three non silent mutation A254G (K85R), T980C (L3…

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. p23-DDX21 SAT

   Plasmid

   #128804

   Purpose

   overexpression of DDX21 SAT mutation in human cells

   Depositor

   Ling-Ling Chen

   Article

   Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

   Insert

   DDX21 SAT mutation
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTH1-SpSrtA

   Plasmid

   #64987

   Purpose

   Expresses the wild type of Streptococcus pyogenes sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   S. pyogenes sortase A
   

   Use

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-81

   Promoter

   T7 promoter

   Available Since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMB66

   Plasmid

   #47946

   Purpose

   Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter

   Depositor

   Mike Boxem

   Article

   Waaijers et al Genetics. 2013 Aug 26.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

   Expression

   Worm

   Mutation

   Promoter

   hsp-16.48

   Available Since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. Venus-Bik-DD-pEGFP-C1

   Plasmid

   #177429

   Purpose

   To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, phosphomimetic mutant. T33D and S35D mutations made to Addgene #166737.

   Depositor

   David Andrews

   Insert

   Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Threonine 33 and Serine 35 replaced with aspartic…

   Promoter

   CMV

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE30-TEV-pro-conA

   Plasmid

   #159527

   Purpose

   Expresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.

   Depositor

   Joshua Mylne

   Article

   Nonis et al bioRxiv. 2020.

   Insert

   Canavalia ensiformis pro-concanavalin A
   

   Use

   Tags

   Six-Histidine tag and tobacco etch virus (TEV) pr…

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPL5628_TEF1*-Cre_MET15

   Plasmid

   #60933

   Purpose

   Constitutive expression vector for Cre-recombinase containing MET15 selection marker.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   Cre
   

   Use

   Tags

   Driven from a mutant TEF1 promoter with 0.16x act…

   Expression

   Yeast

   Mutation

   Promoter

   mutant TEF1

   Available Since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. p23-DDX21 DEV

    Plasmid

    #128805

    Purpose

    overexpression of DDX21 DEV mutation in human cells

    Depositor

    Ling-Ling Chen

    Article

    Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

    Insert

    DDX21 DEV mutation
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. JP1520 PIK3CA E545K HA

    Plasmid

    #14571

    Depositor

    Joan Brugge

    Article

    Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-100

    Insert

    PIK3CA E545K (PIK3CA Bovine)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    E545K

    Promoter

    Available Since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

12. R-G324D-(Kin-8)

    Plasmid

    #28179

    Depositor

    Bernard Schimmer

    Article

    Olson et al Mol Endocrinol. 1993 Apr . 7(4):477-87.

    Insert

    Prkar1a (PRKAR1A Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G324D

    Promoter

    Available Since

    May 12, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-optoB2-SS-YFP

    Plasmid

    #75270

    Purpose

    Arrestin independent version of optoB2 with serine mutations at S362A,S363G

    Depositor

    Michael Bruchas

    Article

    Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

    Insert

    Arrestin independent version of optoB2
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    serine mutations at S362A,S363G

    Promoter

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiCRISPRv2-Blast-mU6

    Plasmid

    #206806

    Purpose

    pLentiCRISPRv2 variant with mU6 promoter driving the sgRNA, and a Blasticidin resistance cassette

    Depositor

    Jolanda Van Leeuwen

    Article

    Unlu et al Genome Med. 2023 Oct 12;15(1):78. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMyc0ElbLuc

    Plasmid

    #53242

    Purpose

    reporter plasmid without myc cDNA binding sites

    Depositor

    Roger Davis

    Article

    Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

    Insert

    pMycOElbLuc (MYC Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pDREV-2

    Plasmid

    #26752

    Depositor

    Rolf Zeller

    Article

    Osterwalder et al Nat Methods. 2010 Oct 17

    Insert

    H2B-Venus CDS (H2BC3 Human)
    

    Use

    Cre/Lox; Flp/frt; dre/rox

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-HemmarI2

    Plasmid

    #112815

    Purpose

    gateway cloning destination vector for expressing enhancer driven CD4-IFP2.0-T2A-HO1

    Depositor

    Chun Han

    Article

    Poe et al Genetics. 2018 Nov 30. pii: genetics.118

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMB62

    Plasmid

    #47944

    Purpose

    Expresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

    Expression

    Worm

    Mutation

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBA1032

    Plasmid

    #189580

    Purpose

    T4soc deletion HR vector 250bp HR Arms (T4 phage editing)

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Insert

    T4soc Deletion Locus 250bp Homology Arms
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Encodes for a full deletion of T4soc

    Promoter

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCh-LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)

    Plasmid

    #165030

    Purpose

    Express cpFRB2-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

    Depositor

    Yubin Zhou

    Article

    Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

    Insert

    LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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