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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,978 results for: LEA

Showing: 10601 - 10650 of 15978 results

  1. pGBW-m4046346

    Plasmid
    #145390
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145390-plasmid-map-sequence-id-298322

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  3. wt Cus2p

    Plasmid
    #73046
    Purpose
    Wild-type S. cerevisiae Cus2p gene for recombinant expression and crystallization
    Depositor
    Aaron Hoskins
    Article
    Rodgers et al RNA. 2015 Dec 2.
    Insert
    CUS2p (CUS2 Budding Yeast)
    Tags
    6x His tag fused to TEV cleavage site
    Expression
    Bacterial and Yeast
    Promoter
    T7
    Available Since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    73046-plasmid-map-sequence-id-150790

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  6. pST50Trc2-CBPDHFR

    Plasmid
    #63954
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Calmodulin Binding Peptide (CBP)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63954-plasmid-map-sequence-id-177360

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  7. 5I3L (DPF3)

    Plasmid
    #79522
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DPF3 (DPF3 Human)
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    June 16, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    79522-plasmid-map-sequence-id-150157

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  8. pST50Trc4-CBPDHFR

    Plasmid
    #63990
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Calmodulin Binding Peptide (CBP)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63990-plasmid-map-sequence-id-177396

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  11. pMTB-Multibow-hO

    Plasmid
    #60996
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear mKO
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    mKO
    Use
    Zebrafish
    Tags
    histone 2B tag
    Promoter
    Bactin2
    Available Since
    Jan. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. pCMV-SpCas9-VQR-P2A-EGFP (LRF199)

    Plasmid
    #242664
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VQR-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    pCMV-SpCas9-VQR-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VQR mutations in SpCas9(D1135V/R1335Q/T1337R)
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242664-plasmid-map-sequence-id-488971

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  14. pUC-GW-Kan-VPC

    Plasmid
    #244163
    Purpose
    Reporter for human p38alpha activation state by FRET
    Depositor
    Thomas Kuhlman
    Insert
    MAPK14/p38a (MAPK14 Human)
    Use
    Synthetic Biology
    Tags
    mCerulean3 and mVenus
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    Sept. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    244163-plasmid-map-sequence-id-487534

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  16. pQdCas12a.luxR(mut)-sggfp(B)

    Plasmid
    #236041
    Purpose
    The plasmid pQdCas12a.luxR(mut)-sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    Use
    CRISPR
    Promoter
    quorum sensing promoter
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236041-plasmid-map-sequence-id-469952

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  17. pQdCas12a.luxR(mut)-sggfp(A)

    Plasmid
    #236039
    Purpose
    The plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    Use
    CRISPR
    Promoter
    quorum sensing promoter
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236039-plasmid-map-sequence-id-469911

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Showing: 10601 - 10650 of 15978 results