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Showing: 10601 - 10650 of 77165 results

  10. CPH3737_GST-TEV-Spt6p-tSH2-1223-1453

    Plasmid
    #227087
    Purpose
    Bacterial expression of K1355A/K1435A double-mutant GST-tagged Spt6 tSH2 domain (1223-1453)
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Use
    Tags
    GST-TEV_cleavage_site
    Expression
    Bacterial
    Mutation
    K1355A, K1435A
    Promoter
    T7
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    227087-plasmid-map-sequence-id-470399

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  11. pAG416-GAL-SUP35

    Plasmid
    #181705
    Purpose
    Galactose inducible expression of SUP35
    Depositor
    Alejandro Chavez
    Insert
    SUP35 (SUP35 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    181705-plasmid-map-sequence-id-466832

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  12. pdCas9-tdTomato

    Plasmid
    #232631
    Purpose
    catalytically inactive dCas9 for localization; labeled with tdTomato
    Depositor
    Karsten Rippe
    Insert
    dCas9-tdTomato
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV (TATA box removed)
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232631-plasmid-map-sequence-id-462150

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  13. psBIG1a

    Plasmid
    #229957
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    229957-plasmid-map-sequence-id-461574

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  14. psBIG1b

    Plasmid
    #229958
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229958-plasmid-map-sequence-id-461279

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  15. psBIG1d

    Plasmid
    #229960
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229960-plasmid-map-sequence-id-461267

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  16. psBIG1e

    Plasmid
    #229961
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229961-plasmid-map-sequence-id-461266

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  17. psBIG1c

    Plasmid
    #229959
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229959-plasmid-map-sequence-id-461263

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  18. pLS406

    Plasmid
    #231078
    Purpose
    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker URA3
    Depositor
    Sahand Jamal Rahi
    Type
    Empty backbone
    Use
    Integrating
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231078-plasmid-map-sequence-id-460262

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  19. pLS405

    Plasmid
    #231077
    Purpose
    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker LEU2
    Depositor
    Sahand Jamal Rahi
    Type
    Empty backbone
    Use
    Integrating
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231077-plasmid-map-sequence-id-459803

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  20. pLS404

    Plasmid
    #231076
    Purpose
    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker TRP1
    Depositor
    Sahand Jamal Rahi
    Type
    Empty backbone
    Use
    Integrating
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231076-plasmid-map-sequence-id-459800

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  21. pLS408

    Plasmid
    #231079
    Purpose
    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker natMX6
    Depositor
    Sahand Jamal Rahi
    Type
    Empty backbone
    Use
    Integrating
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231079-plasmid-map-sequence-id-459799

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  22. pLS403

    Plasmid
    #231075
    Purpose
    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker HIS3
    Depositor
    Sahand Jamal Rahi
    Type
    Empty backbone
    Use
    Integrating
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231075-plasmid-map-sequence-id-459792

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  27. CPH3747_GST-TEV-Spt6p-tSH2-1223-1452

    Plasmid
    #227086
    Purpose
    Bacterial expression of R1282H single-mutant GST-tagged Spt6 tSH2 domain (1223-1452)
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Use
    Tags
    GST-TEV_cleavage_site
    Expression
    Bacterial
    Mutation
    R1282H
    Promoter
    T7
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227086-plasmid-map-sequence-id-451101

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  28. CPH3375_SF_Spt6p-tSH2-1223-1451_NLS

    Plasmid
    #227084
    Purpose
    Gal1p dependent budding yeast expression of tandem-affinity tagged spt6p tSH2 domain (1123-1451) localized to nucleus
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Use
    Tags
    STREPii-FLAG and SV40 NLS 1
    Expression
    Yeast
    Mutation
    Promoter
    Gal1-10
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227084-plasmid-map-sequence-id-451056

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  29. CUP1-∆ssCPY*-mScarletI

    Plasmid
    #221157
    Purpose
    Fluorescent reporter for cytoplasmic aggregates
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Use
    Tags
    mScarletI
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221157-plasmid-map-sequence-id-450959

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  30. CUP1-∆ssCPY*-mNeonGreen

    Plasmid
    #221156
    Purpose
    Fluorescent reporter for cytoplasmic aggregates
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Use
    Tags
    mNeonGreen
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221156-plasmid-map-sequence-id-450957

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  31. L1T18 - T2PsB/p35S preswitched (mTagBFP2, mCherry, NeonGreen)

    Plasmid
    #219690
    Purpose
    Level 1 preswitched History-dependent target, switch with Bxb1, p35S promoter, and output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2PsB/p35S (mTagBFP2, mCherry, NeonGreen)
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219690-plasmid-map-sequence-id-435857

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  32. m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP

    Plasmid
    #211343
    Purpose
    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211343-plasmid-map-sequence-id-415647

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  35. pAMW-Wnk

    Plasmid
    #203978
    Purpose
    Myc6 Wnk under actin promoter for cell culture
    Depositor
    Andreas Jenny
    Insert
    Wnk (Wnk Fly)
    Use
    Tags
    6xMyc
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203978-plasmid-map-sequence-id-403206

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  36. FNF-CAG-L-3xSV40pA-L-tdTomato shuttle vector

    Plasmid
    #182346
    Purpose
    PacI-flanked CAG-LSL expression cassette with a FNF for positive selection
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Mouse Targeting; Flp / frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182346-plasmid-map-sequence-id-358221

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  37. pB2HoptX

    Plasmid
    #163832
    Purpose
    Inactive two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTP1B
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    MidT Substrate Y4F
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163832-plasmid-map-sequence-id-337598

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  38. pNSU299_RPL39

    Plasmid
    #166078
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL39 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of RPL39 (Overlaps with 5'UTR and first base of gene) (RPL39 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166078-plasmid-map-sequence-id-330349

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  39. pNSU299_YDR344C

    Plasmid
    #166072
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA targeting intergenic site near HXT6 (within YDR344C) for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Intergenic site near HXT6 (in YDR344C, possible dubious ORF)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166072-plasmid-map-sequence-id-329603

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  40. pNSU299_TDH3

    Plasmid
    #166084
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of TDH3 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of TDH3 (TDH3 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166084-plasmid-map-sequence-id-329123

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  41. pDGB3 omega1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1657)

    Plasmid
    #160640
    Purpose
    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).
    Depositor
    Diego Orzaez
    Insert
    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    Use
    CRISPR
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, 35S
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    160640-plasmid-map-sequence-id-321670

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  45. pKLV-gRNA-BTRC-MGMT_2

    Plasmid
    #136416
    Purpose
    Lentiviral expression of gRNAs targeting intron 2 of human BTRC and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.
    Depositor
    Massimo Squatrito
    Insert
    U6_sgRNA(BTRC)_U6_sgRNA(MGMT)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    136416-plasmid-map-sequence-id-271757

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Showing: 10601 - 10650 of 77165 results