We narrowed to 4,862 results for: Mela

1. pAc5.1B-lambdaN-HA-DmTral-R21ES13AI15A_C

   Plasmid

   #146079

   Purpose

   Insect Expression of DmTral-R21ES13AI15A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-R21ES13AI15A (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   three silent mutations A198C, A387G, A1437G and 4…

   Promoter

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAc5.1B-lambdaN-HA-DmTral-R42EF44A_C

   Plasmid

   #146080

   Purpose

   Insect Expression of DmTral-R42EF44A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-R42EF44A (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   three silent mutations A198C, A387G, A1437G and 4…

   Promoter

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAc5.1B-lambdaN-HA-DmTral-S13AI15A_C

   Plasmid

   #146081

   Purpose

   Insect Expression of DmTral-S13AI15A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-S13AI15A (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   three silent mutations A198C, A387G, A1437G and 4…

   Promoter

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAc5.1B-EGFP-DmTral_394-543-V5His6_D

   Plasmid

   #146134

   Purpose

   Insect Expression of DmTral_394-543

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral_394-543 (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   two silent mutations (nucleotides A198C, A387G) a…

   Promoter

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KR42EF44A-V5His6_C

   Plasmid

   #146071

   Purpose

   Insect Expression of DmTral-LSmR21EE23KR42EF44A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSmR21EE23KR42EF44A (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   R21E, E23K, R42E, F44A. Additionally, one silent …

   Promoter

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAc5.1B-EGFP-DmTral-R42EF44A_C

   Plasmid

   #146061

   Purpose

   Insect Expression of DmTral-R42EF44A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-R42EF44A (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   R42E, F44A. Additionally, two silent mutations A1…

   Promoter

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C

   Plasmid

   #146049

   Purpose

   Insect Expression of DmTral-LSmR21EE23KS13AI15A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSmR21EE23KS13AI15A (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   S13A, I15A, R21E, E23K, one silent mutation A198C…

   Promoter

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1B-lambdaN-HA-DmTral-FDF-ADA_C

   Plasmid

   #146039

   Purpose

   Insect Expression of DmTral-FDF-ADA

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

   Insert

   DmTral-FDF-ADA (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   FDF to ADA (407-409) mutation. Additionally, thre…

   Promoter

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Lenti_CRISPRi_sgAR10

   Plasmid

   #167003

   Purpose

   Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

   Depositor

   Gian-Paolo Dotto

   Article

   Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

   Insert

   AR Promoter sgRNA 10 (AR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pU6-chiRNA_dCrkgRNA1

    Plasmid

    #131139

    Purpose

    Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    dCrk gRNA1 (Crk Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pU6-chiRNA_dCrkgRNA2

    Plasmid

    #131140

    Purpose

    Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    dCrk gRNA1 (Crk Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDF1-MCS2-EF1-Puro-RGS7-E383K

    Plasmid

    #113731

    Purpose

    Express mutant RGS7- E383K- in mamalian cells

    Depositor

    Yardena Samuels

    Article

    Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

    Insert

    RGS7 (RGS7 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    E383K

    Promoter

    CMV

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDF1-MCS2-EF1-Puro-RGS7-R44C

    Plasmid

    #113729

    Purpose

    Express mutant RGS7- R44C- in mamalian cells

    Depositor

    Yardena Samuels

    Article

    Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

    Insert

    RGS7 (RGS7 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDF1-R310_RASA2-EF1-Puro

    Plasmid

    #114097

    Purpose

    Express mutant RASA2 in mamalian cells

    Depositor

    Yardena Samuels

    Article

    Arafeh et al Nat Genet. 2015 Dec;47(12):1408-10. doi:

    Insert

    RASA2 (RASA2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    R310*

    Promoter

    CMV

    Available Since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mei-S332-GFP 5.6kb genomic DNA in Casper4 S123-125-3A

    Plasmid

    #112987

    Purpose

    In P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (S123-125-3A) into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

    Insert

    mei-S332 (mei-S332 Fly)
    

    Use

    P insertion vector

    Tags

    GFP

    Expression

    Mutation

    S123-125-3A

    Promoter

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mei-S332-GFP 5.6kb genomic DNA in Casper4 S123-125-3D

    Plasmid

    #112985

    Purpose

    In P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (S123-125-3D) into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

    Insert

    mei-S332 (mei-S332 Fly)
    

    Use

    P insertion vector

    Tags

    GFP

    Expression

    Mutation

    S123-125-3D

    Promoter

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMAL c2x Tral FL-15D

    Plasmid

    #113010

    Purpose

    For bacterial expression of MBP fusion of full-length Drosophila Tral with Phos-mimic mutations

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length tral (tral Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    15 PNG phosphorylation sites mutated to D

    Promoter

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc-GFP-dSUMOdeltaC

    Plasmid

    #85697

    Purpose

    expression of GFP-SUMO1 delta C in Drosophila cells

    Depositor

    Licio Collavin, Giannino Del Sal

    Article

    Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

    Insert

    SUMO (Sumo Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    non-conjugatable SUMO, lacking the C-terminal gly…

    Promoter

    pAc5

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

19. KHBD00320

    Plasmid

    #34445

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG6376 (E2f1 Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pBABE-hygro-2xMyc-Rac1-P29S

    Plasmid

    #128581

    Purpose

    For mammalian cell expression of P29S mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

    Depositor

    Julian Downward

    Article

    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

    Insert

    Rac1 (Rac1 Mouse)
    

    Use

    Retroviral

    Tags

    2xMyc epitope

    Expression

    Mammalian

    Mutation

    Changed Proline29 to Serine.

    Promoter

    Available Since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge