We narrowed to 7,596 results for: Lif;

1. pcDNA3.1(-)-qmTQ2-ATP

   Plasmid

   #225248

   Purpose

   mammalian expression of qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

   Depositor

   Yasushi Okada

   Article

   Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

   Insert

   qmTQ2-ATP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLH-sgRNA-Sirius-8XMS2

   Plasmid

   #121939

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   sgRNA-Sirius-8XMS2
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. ttAtCas12a+int

   Plasmid

   #182384

   Purpose

   Gives very high editing efficiency in barley. Also works in Brassica oleracea

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al bioRxiv 2022.04.28.489853

   Insert

   LbCas12a coding sequence with D156R and Arabidopsis introns
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D156R + 8 Arabidopsis introns added

   Promoter

   None

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. p159 pPr-luc

   Plasmid

   #8392

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

   Insert

   probasin promoter (Pbsn Rat)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 29, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pBAD/HisB (TIREVOL)-sfGFP

   Plasmid

   #189675

   Purpose

   titratable expression of sfGFP in bacteria

   Depositor

   Daniel Daley

   Article

   Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

   Insert

   sfGFP
   

   Use

   Tags

   His-T7-EK

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPUR-mU6-sgRNA-Sirius-8XPP7

   Plasmid

   #121943

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   sgRNA-Sirius-8XPP7
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6 promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-AncMEcoGII291

   Plasmid

   #205024

   Purpose

   Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   Ancestral M.EcoGII 291
   

   Use

   Tags

   His6x tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-TO-dCas9-P2A-HSA

   Plasmid

   #121936

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   dCas9-P2A-HSA
   

   Use

   CRISPR and Lentiviral

   Tags

   HSA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMU-ACTLr

   Plasmid

   #61193

   Purpose

   Fluorescent reporter for F-actin LifeAct-mCherry expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   LifeAct-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO_[Cox8a]x2_HaloTag7

    Plasmid

    #175529

    Purpose

    Expression of HaloTag7 at the inner membrane of mitochondria in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Cox8a-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-hCDC6p-Venus

    Plasmid

    #212665

    Purpose

    Express Venus under CDC6 promoter

    Depositor

    Yumi Konagaya, Tobias Meyer

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    hCDC6p-Venus
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hCDC6p

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-AncMEcoGII250

    Plasmid

    #205025

    Purpose

    Expressing engineered M.EcoGII with higher thermostability

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    Ancestral M.EcoGII 250
    

    Use

    Tags

    His6x tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Flag FoxO3

    Plasmid

    #14937

    Depositor

    Joan Massague

    Article

    Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

    Insert

    FoxO3 (FOXO3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO_Beta4Gal-HaloTag7

    Plasmid

    #175536

    Purpose

    Expression of HaloTag7 at the at the Gogli apparatus of the mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Beta4Gal-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

    Plasmid

    #175534

    Purpose

    Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    IgKchL_HA-HaloTag7-myc_PDGFRtmb
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGFP-E p27 (CC#631)

    Plasmid

    #15192

    Depositor

    Connie Cepko

    Article

    Dyer et al J Neurosci. 2001 Jun 15. 21(12):4259-71.

    Insert

    p27 (Cdkn1b Mouse)
    

    Use

    Retroviral

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5/FRT/TO_b4g-HaloTag9

    Plasmid

    #175546

    Purpose

    Expression of HaloTag9 at the at the Gogli apparatus of the mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Beta4Gal-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-CyRFP1

    Plasmid

    #84356

    Purpose

    Expresses CyRFP1 fluorescent protein in an empty backbone, under CAG promoter

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    CyRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p155 pCMV-EGFP/beta-gal

    Plasmid

    #8387

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    floxed EGFP
    

    Use

    Cre/Lox; Beta-gal

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLH-sgRNA-Sirius-8XPP7

    Plasmid

    #121940

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-8XPP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge