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twinStrep_aMFx1 (4a)

Plasmid

#238906

Purpose

Plasmid insert contains the aMFx1 and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

twinStrep_aMFx1

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pSIn-H3K9ac Y97F BS

Plasmid

#235132

Purpose

Mutant (negative control) Y97F biosensor for pSin-H3K9ac Full

Depositor

Qin Peng

Article

Li et al Med-X. 2024; 2, 26. doi: 10.1007/s44258-

Insert

BD1Y97F-Ypet-34mer-ECFP-H3

Use

Lentiviral

Expression

Mammalian

Mutation

BD1Y97F

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pUBC(h)-DEST-mKOk

Plasmid

#224830

Purpose

Empty Gateway DEST vector for expressing C-terminal tagged proteins with mKusabiraOrangekand hygromycin selection in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Type

Empty backbone

Tags

mKusabiraOrangek

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-mRuby2

Plasmid

#233155

Purpose

Retroviral expression of fluorescent mRuby2 reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mRuby2

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ265_BasA3G

Plasmid

#225145

Purpose

Gateway entry clone (attL1 & attR5) BasA3G-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

Inserts

BasA3G-zCas9(D10A)-UGI
-

Expression

Plant

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ265_EtA3C

Plasmid

#225146

Purpose

Gateway entry clone (attL1 & attR5) EtA3C-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

Insert

EtA3C-zCas9(D10A)-UGI

Expression

Plant

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ265_DlA3G

Plasmid

#225148

Purpose

Gateway entry clone (attL1 & attR5) DlA3G-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

Insert

DlA3G-zCas9(D10A)-UGI

Expression

Plant

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ265_LoA3GX1

Plasmid

#225149

Purpose

Gateway entry clone (attL1 & attR5) LoA3GX1-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

Insert

LoA3GX1-zCas9(D10A)-UGI

Expression

Plant

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-giantin-ATG_donor

Plasmid

#222316

Purpose

Perform knock-in by homologous recombination in giantin/GOLGB1 gene.

Depositor

Franck Perez

Article

Fourriere et al J Cell Sci. 2016 Sep 1;129(17):3238-50.

Insert

EGFP and homology arms for giantin/GOLGB1

Use

CRISPR

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB602-MpPHOT-3xFLAG

Plasmid

#228474

Purpose

MpPHOT-3xFLAG under the control of the cauliflower mosaic virus (CaMV) 35S promoter (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

MpPHOT-3xFLAG

Expression

Bacterial and Plant

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL194

Plasmid

#222225

Purpose

Expresses site 1/Cas9/Eco1 RT in HIS3 locus.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

site 1/Cas9/Eco1 RT

Use

CRISPR

Expression

Yeast

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL195

Plasmid

#222226

Purpose

Expresses site 2/Cas9/Eco1 RT in HIS3 locus.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

site 2/Cas9/Eco1 RT

Use

CRISPR

Expression

Yeast

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL368

Plasmid

#222227

Purpose

Expresses site 3/Cas9/Eco1 RT in HIS3 locus.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

site 3/Cas9/Eco1 RT

Use

CRISPR

Expression

Yeast

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCIwt-PRM-Wasabi

Plasmid

#195553

Purpose

Always-on expression of mWasabi reporter with transcription activation by wildtype CI

Depositor

Mikhail Shapiro

Article

Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

Inserts

mWasabi
CIwt

Tags

6x His and AANDENYADAS degradation tag

Expression

Bacterial

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSECB sgCASP9

Plasmid

#211525

Purpose

Deletes CASP9

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgCASP9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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BPK1520 sgFADD

Plasmid

#211527

Purpose

Deletes FADD

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgPIDD

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-pOTC-GFP

Plasmid

#205724

Purpose

Expression of GFP fused with pOTC, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

pOTC-GFP

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgUCKL1

Plasmid

#211523

Purpose

Deletes UCKL1

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgUCKL1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgUCK2

Plasmid

#211522

Purpose

Deletes UCK2

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgUCK2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgUGP2

Plasmid

#211521

Purpose

Deletes UGP2

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgUGP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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