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pEMS1991

Plasmid

#49122

Purpose

AAV plasmid with FEV (Ple67) promoter driving expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

ssAAV-Ple67-iCre-WPRE

Use

AAV

Expression

Mammalian

Promoter

FEV

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 N

Plasmid

#158079

Purpose

Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 nucleocapsid (N )

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP

Plasmid

#188778

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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Artichoke

Plasmid

#73320

Purpose

Artichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

SFFV and EF1a

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLVNP3.0-PEmax

Plasmid

#206883

Purpose

Expresses FLAG-tagged PEmax fused to Gag through a linker sequence

Depositor

Jacob Giehm Mikkelsen

Article

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Insert

PEmax

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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Frt-hspB5

Plasmid

#63096

Purpose

Expression of HSPB5 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb5 (CRYAB Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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AbVec2.0-mIgkc

Plasmid

#127157

Purpose

Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), kappa constant region

Depositor

Hedda Wardemann

Article

Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

Insert

Igkc (Igkc Mouse)

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pGlomyc-Grhl3

Plasmid

#172869

Purpose

Mammalian expression vector with myc-tagged mouse Grhl3 coding sequence

Depositor

Renee van Amerongen

Article

Mourao et al bioRxiv 2021.06.22.449461

Insert

Grhl3 (Grhl3 Mouse)

Tags

myc

Expression

Mammalian

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFV2/myc-His

Plasmid

#127491

Purpose

Expresses myc-tagged NDUFV2 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFV2 (NDUFV2 Human)

Tags

myc-His

Expression

Mammalian

Promoter

CMV

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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LentiGuide-hygro

Plasmid

#160090

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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PDG459 V3

Plasmid

#226958

Purpose

CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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PCMV10-RBPJ

Plasmid

#84601

Purpose

Mammalian expression of FLAG tagged human RBPJ

Depositor

Yanan Yang

Article

Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

Insert

RBPJ (RBPJ Human)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 E

Plasmid

#158080

Purpose

Expresses SARS-CoV-2 envelope protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 envelope (E )

Expression

Mammalian

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 M

Plasmid

#158078

Purpose

Expresses SARS-CoV-2 membrane protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 matrix (M )

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Str-KDEL-SBP-EGFP-LAMP1

Plasmid

#222317

Purpose

Synchronize the trafficking of LAMP1 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1 fused to SBP-EGFP (LAMP1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pPPI4-SNAP-CTLA-4-His6

Plasmid

#223584

Purpose

Protein purification of human CTLA4 with SNAP and 6xHis tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CTLA4 (CTLA4 Human)

Tags

His6 and SNAP

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.MTF2

Plasmid

#125169

Purpose

Expresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

MTF2 (MTF2 Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-LAMP2

Plasmid

#222321

Purpose

Synchronize the trafficking of LAMP2 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP2 fused to SBP-EGFP (LAMP2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-dSV40-CTLA-4-mGFP

Plasmid

#223594

Purpose

Lentiviral expression of human CTLA4 with mGFP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CTLA4 (CTLA4 Human)

Use

Lentiviral; mammalian expression

Tags

mGFP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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AbVec2.0-mIghg2b

Plasmid

#127155

Purpose

Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2b isotype

Depositor

Hedda Wardemann

Article

Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

Insert

Ighg2b (Ighg2b Mouse)

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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