We narrowed to 6,132 results for: cat.2

1. FEMP

   Plasmid

   #191973

   Purpose

   FRET-based indicator of ER-mitochondria proximity (FEMP)

   Depositor

   Luca Scorrano

   Article

   Naon et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

   Insert

   AkapYFP-Tav2a-CFP-sac1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. MmSphk2

   Plasmid

   #118598

   Purpose

   Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in mice

   Depositor

   Kevin Lynch

   Article

   Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

   Insert

   Mus musculus Sphingosine Kinase 2 (Sphk2 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   GAL1, 10

   Available since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. L1-1147

   Plasmid

   #13267

   Depositor

   Vance Lemmon

   Article

   Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

   Insert

   L1CAM (L1CAM Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Truncated in the juxtamembrane region

   Promoter

   Available since

   Dec. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL

   Plasmid

   #216253

   Purpose

   FRET-based calcium sensor located in the ER lumen, Tet-inducible

   Depositor

   Edward Avezov

   Article

   Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

   Insert

   ER-targeted D4 second generation cameleon (FRET calcium sensor)
   

   Use

   Lentiviral

   Tags

   REAch and mTurquoise

   Expression

   Mutation

   Promoter

   TRE3G

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSCL.109

   Plasmid

   #184978

   Purpose

   Test effect of extending a1/a2 on LYP1 editing rates in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 27 v1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   LYP1 donor E27stop, a1/a2 length extended to 27 bp

   Promoter

   Gal7

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSCL.111

   Plasmid

   #184980

   Purpose

   Test effect of extending a1/a2 on TRP2 editing rates in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 27 v1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   TRP2 donor E64stop, a1/a2 length extended to 27 bp

   Promoter

   Gal7

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSC39

   Plasmid

   #104813

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Medtr2g101180 (Cop-like). Also expresses Cas9 from Gmubi promoter.

   Depositor

   Nevin Young

   Article

   Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

   Insert

   Medtr2g101180
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSC25

   Plasmid

   #104799

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Medtr2g101040 (Mel1). Also expresses Cas9 from Gmubi promoter from Gmubi promoter

   Depositor

   Nevin Young

   Article

   Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

   Insert

   Medtr2g101040
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSC24

   Plasmid

   #104798

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Medtr2g101120, Medtr2g101130 (Acre2). Also expresses Cas9 from rolD promoter from AtUBQ10 promoter

   Depositor

   Nevin Young

   Article

   Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

   Insert

   Medtr2g101120, Medtr2g101130
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSC22

    Plasmid

    #104796

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Medtr2g101120, Medtr2g101130 (Acre1). Also expresses Cas9 from Gmubi promoter from AtUBQ10 promoter

    Depositor

    Nevin Young

    Article

    Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

    Insert

    Medtr2g101120, Medtr2g101130
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. XE120 CAMKII-K42M 1-271-CS2+

    Plasmid

    #16734

    Depositor

    Randall Moon

    Article

    Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

    Insert

    CAMKII-K42M 1-271 (Camk2a Rat)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    C-terminal truncation of K42M kinase

    Promoter

    Available since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

12. PP023 - CAG::QuasAr6a-TS-dmCitrine-TSx3-ER2-p2a- jRGECO1a-CAAX

    Plasmid

    #182063

    Purpose

    Co-expression of Arch-based voltage indicator (QuasAr6a) and membrane-localized calcium reporter (jRGECO1a-CAAX)

    Depositor

    Adam Cohen

    Article

    Landau et al eLife. 2022 Mar 23;11. pii: 76993. doi:

    Insert

    QuasAr6a-TS-dmCitrine-TSx3-ER2-p2a- jRGECO1a-CAAX
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. L1-1180

    Plasmid

    #13265

    Depositor

    Vance Lemmon

    Article

    Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

    Insert

    L1CAM (L1CAM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Truncated after RSLE region

    Promoter

    Available since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

14. L1-1176

    Plasmid

    #13264

    Depositor

    Vance Lemmon

    Article

    Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

    Insert

    L1CAM (L1CAM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Truncated before RSLE region

    Promoter

    Available since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pG2J

    Plasmid

    #102882

    Purpose

    Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.

    Depositor

    Matthias Heinemann

    Article

    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

    Inserts

    OsTIR1
    mGFP-AID
    

    Use

    Tags

    AID(71-114)

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 and TEF1

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP

    Plasmid

    #154139

    Purpose

    Expresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    dCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP
    

    Use

    Tags

    3x FLAG and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. scFv-GCN4-DNMT3a-DNMT3l

    Plasmid

    #154140

    Purpose

    Expresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
    

    Use

    Tags

    HA and sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMP926

    Plasmid

    #135001

    Purpose

    m6A Reader-Writer. Propagates Gm6ATC along GATC arrays as well as across cell generations. pUBC-DpnI(aa146-254)-3xFLAG-Dam(R95A) pGK-HygroR

    Depositor

    Ahmad Khalil

    Article

    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

    Insert

    pUBC-DpnI(aa146-254)-3XFLAG-Dam(R95A) pGK-HygroR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xFLAG, Dam(R95A), and DpnI(aa146-254)

    Expression

    Mammalian

    Mutation

    DpnI domain truncated to aa146-254. Dam protein m…

    Promoter

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-FLEX-tmeGFP-IRES-nls-mRuby2

    Plasmid

    #63060

    Purpose

    Expresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    nls-mRuby2
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. sgRNA4_NANOG

    Plasmid

    #64156

    Purpose

    Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA4_NANOG (NANOG Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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