We narrowed to 6,888 results for: hwa

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pET20b-mPTER-Y65F

Plasmid

#222203

Purpose

Bacterial expression of mouse PTER-Y65F protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Tags

6xHis and Strep

Expression

Bacterial

Mutation

Y65F

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-Y263F

Plasmid

#222204

Purpose

Bacterial expression of mouse PTER-Y263F protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Tags

6xHis and Strep

Expression

Bacterial

Mutation

Y263F

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNK189

Plasmid

#219743

Purpose

MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Homo sapiens

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

mutant of fungal hispidin-3-hydroxylase

Use

Synthetic Biology

Mutation

D37E, V181I, S323M, M385K

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK4169

Plasmid

#219744

Purpose

MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Pichia pastoris

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

mutant of fungal hispidin-3-hydroxylase

Use

Synthetic Biology

Mutation

D37E, V181I, S323M, M385K

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7183

Plasmid

#219760

Purpose

MoClo-compatible Level 1 for expression PzPKS2 in plants (N.benthamiana, BY2 cells)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

PzPKS2

Expression

Plant

Promoter

p35s_0.4kb - 5'UTR TMV omega

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7180

Plasmid

#219762

Purpose

MoClo-compatible Level 1 for expression HmS in plants (N.benthamiana, BY2 cells)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

HmS

Expression

Plant

Promoter

p35s_0.4kb - 5'UTR TMV omega

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR ins5 YD/FL-FLAG

Plasmid

#214706

Purpose

Mammalian expression of human CALR ins5 YD/FL-FLAG

Depositor

Norio Komatsu

Article

Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

Insert

CALR ins5 YD/FL-FLAG (CALR Human)

Use

Retroviral

Tags

FLAG

Promoter

MSCV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-AtSLAC1 8D

Plasmid

#212948

Purpose

Vector used for expression of AtSLAC1 8D in mammalian cell

Depositor

Sangho Lee

Article

Lee et al Nat Commun. 2023 Nov 14;14(1):7345. doi:

Insert

Slow anion channel-associated 1 (OZS1 Mustard Weed)

Expression

Mammalian

Mutation

S59D/T62D/S65D/S86D/S107D/S124D/S146D/S152D

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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METTL3

Plasmid

#155725

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

METTL3 (METTL3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

H330Q compared to BC052244, no mutations compared…

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EDC3

Plasmid

#155561

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

EDC3 (EDC3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV Flag-LRRK2 K17A/K18A/R1441G

Plasmid

#186013

Purpose

Flag-LRRK2 K17A/K18A/R1441G

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

Flag-LRRK2 K17A/K18A/R1441G (LRRK2 Human)

Expression

Mammalian

Mutation

See depositor's comments below.

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 Myc-tagged PSK (K57A)

Plasmid

#197150

Purpose

Expression of kinase-deficient MYC-tagged PSK1-alpha (K57A) / TAOK2 (K57A) in mammalian cells

Depositor

Jonathan Morris

Article

Moore et al J Biol Chem. 2000 Feb 11;275(6):4311-22.

Insert

TAOK2 (TAOK2 Human)

Tags

Myc

Expression

Mammalian

Mutation

Changed K 57 to A

Promoter

SV40

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shCALM2-1

Plasmid

#193699

Purpose

Constitutive lentiviral expression of CALM2 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

CALM2 (CALM2 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shCALM2-2

Plasmid

#193700

Purpose

Constitutive lentiviral expression of CALM2 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

CALM2 (CALM2 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shCALM2-3

Plasmid

#193701

Purpose

Constitutive lentiviral expression of CALM2 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

CALM2 (CALM2 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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DDX27

Plasmid

#155513

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX27 (DDX27 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBoBi-hPEN2 20A

Plasmid

#183639

Purpose

Lentiviral expression of a PEN2 mutant showing impaired affinity with ATP6AP1.

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 (PSENEN Human)

Use

Lentiviral

Tags

HA

Mutation

residues 27, 28, 30, 31, 34, 38, 42, 43, 57, 58, …

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pInducer10-SAE1_sh3

Plasmid

#168985

Purpose

Expresses RFP cDNA and SAE1 shRNA 3 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

SUMO1 Activating Enzyme Subunit 1 (SAE1 Human)

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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MSCVhyg-HA-FLAG_Ubc9_C93A

Plasmid

#164937

Purpose

Expresses FLAG & HA tagged human Ubiquitin Conjugating Enzyme 9 (UBC9) _C93A mutant cDNA

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

human UBC9 (UBE2I Human)

Use

Retroviral

Tags

FLAG & HA tags

Promoter

PGK promoter

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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deltaVP1 + VP2C-GFP-MIOX

Plasmid

#166682

Purpose

Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Inserts

VP2C-GFP-MIOX
Murine polyomavirus deltaVP1 (NLS deletion mutant)

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL1 and GAL10

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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