We narrowed to 6,642 results for: hwa

1. pdCas9-M-1E4

   Plasmid

   #73436

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1E4.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 1E4 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   March 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pdCas9-M-5F5

   Plasmid

   #73432

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 5F5.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 5F5 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Lenti-LucA

   Plasmid

   #174047

   Purpose

   Expresses mALG8 (ITYTWTRL) antigen as a fusion to luciferase and Cre recombinase

   Depositor

   Tyler Jacks

   Article

   Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.

   Insert

   LucA
   

   Use

   Cre/Lox, Lentiviral, and Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   ALG8 alanine 506 to threonine

   Promoter

   Human Ubiquitin C

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Lenti-LucL

   Plasmid

   #174048

   Purpose

   Expresses mLAMA4 (VGFNFRTL) antigen as a fusion to luciferase and Cre recombinase

   Depositor

   Tyler Jacks

   Article

   Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.

   Insert

   LucL
   

   Use

   Cre/Lox, Lentiviral, and Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   LAMA4 glycine 1254 to valine

   Promoter

   Human Ubiquitin C

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONR223-HK2

   Plasmid

   #23854

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   HK2 (HK2 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pLV x EF1a_EKAREN5-ires-puro

   Plasmid

   #167821

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN5
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR223-TBK1

   Plasmid

   #23851

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Caspase-3 (C163A)-pcw107-V5

   Plasmid

   #64633

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   CASP3 (transcript variant beta) (CASP3 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   C163A

   Promoter

   PGK

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pDONR223-ZAP70

   Plasmid

   #23887

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   ZAP70 (ZAP70 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR223-PIM1

    Plasmid

    #23692

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PIM1 (PIM1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR223-PRKAG3

    Plasmid

    #23549

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PRKAG3 (PRKAG3 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pT3-EF1a-YapS127A

    Plasmid

    #46049

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    S127A

    Promoter

    EF1a

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pDONR223-NTRK2

    Plasmid

    #23883

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    NTRK2 (NTRK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTAHR TH-p2a-TD:Tomato (floxed selection Puro)

    Plasmid

    #135814

    Purpose

    Fluorescent reporter for knock-in of TdTomato into the TH locus

    Depositor

    Tim Ahfeldt, Lee Rubin

    Article

    Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

    Insert

    TH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)
    

    Use

    Tags

    p2a-TdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR223-PLK2

    Plasmid

    #23691

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PLK2 (PLK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

16. MEK1 (S218D,S222D)-pcw107

    Plasmid

    #64604

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S218D, S222D

    Promoter

    PGK

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR223-PIK3C2G

    Plasmid

    #23483

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PIK3C2G (PIK3C2G Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    C437T,3BP-del,1MM

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pDONR223-PTK2

    Plasmid

    #23902

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PTK2 (PTK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR223-PIK3CB

    Plasmid

    #23542

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PIK3CB (PIK3CB Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    I6L

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pDONR223-PIK3CG

    Plasmid

    #23856

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PIK3CG (PIK3CG Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    1MM

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only