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pEZY19

Plasmid

#18668

Purpose

pET19b converted Gateway Destination vector for expressing His tagged fusion proteins in bacteria. See note in comments section below regarding the mutated enterokinase site.

Depositor

Yu-Zhu Zhang

Article

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Use

Gateway destination vector

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

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pAB152

Plasmid

#101663

Purpose

Opto-T7RNAP*(563-F2)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563-F2)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCD185

Plasmid

#113317

Purpose

dCas9, MCP-SoxS_R93A

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9 and MCP-SoxS_R93A

Use

CRISPR

Expression

Bacterial

Mutation

R93A

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pACYC-PLlambda-alkSm4-3

Plasmid

#44383

Depositor

Hal Alper

Article

Reed et al J Biotechnol. 2012 Apr 15;158(3):75-9. d

Insert

AlkSm4-3

Use

Synthetic Biology

Mutation

Q410I, S470T, K546E, E775D

Promoter

PLlambda

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pMYO

Plasmid

#34626

Depositor

Jesse Rinehart, Dieter Söll

Article

Park et al Science. 2011 Aug 26;333(6046):1151-4.

Insert

Myoglobin

Tags

6xHIS

Expression

Bacterial

Mutation

codon-optimized

Promoter

lpp

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pGL0_7 [pSa ORI]

Plasmid

#198923

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSa ORI

Use

Synthetic Biology

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pDest-663 (attR4-attR2)

Plasmid

#161884

Purpose

Multisite Gateway (attR4-attR2) vector for lentivirus production

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pBXCA3H

Plasmid

#47072

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), Avi-tag, and…

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pDonorGFP(Ptr)

Plasmid

#175578

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

Mini-Tn, GFP cassette

Expression

Bacterial

Mutation

NO

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pFF745

Plasmid

#61450

Purpose

IPTG-inducible SCRIBE_kanR(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_kanR(ON)

Use

Synthetic Biology

Promoter

pLacO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0296

Plasmid

#162305

Purpose

Level 0 CDS part for MoClo assembly, AATG-TTCG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

CDS, superfolder GFP, no stop codon

Use

Synthetic Biology

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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FusX DdCBE DddA6 1397N

Plasmid

#221934

Purpose

Backbone plasmid for FusX-based assembly of DdCBE arm with DddA6 1397N

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

DddA: Q1310R, S1330I, T1380I

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FusX DdCBE DddA11 1397N

Plasmid

#221935

Purpose

Backbone plasmid for FusX-based assembly of DdCBE arm with DddA11 1397N

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

DddA: S1330I, A1341V, N1342S, E1370K, T1380I

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FusX alphaDdCBE DddA6-11 1397C

Plasmid

#221938

Purpose

Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA6/11 1397C

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

TALE: Q231R, W232G, S233A | DddA: T1413I

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pNCS

Plasmid

#17362

Depositor

Stephen Goff

Article

Yueh et al J Virol. 2003 Feb . 77(3):1820-9.

Insert

MMLV proviral DNA

Available Since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
pUPD2-HA-LRR-NEL-C (B3-B4)

Plasmid

#211860

Purpose

CDS for E3-DART ligase sequence in domestication vector

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Tags

HA

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pNR188

Plasmid

#79543

Purpose

Output plasmid: contains 16-state register made up of TP901, BxbI, and A118 recognition sites.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFF753

Plasmid

#61453

Purpose

IPTG-inducible msd(wt)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd(wt)

Use

Synthetic Biology

Promoter

pLacO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pRec ΔLacI

Plasmid

#104575

Purpose

Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR constitutively.

Depositor

Harris Wang

Article

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PTet-cas12, tetR

Use

Synthetic Biology

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEMF037

Plasmid

#157634

Purpose

Batis maritima MHT

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Insert

MHT

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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