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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 4301 - 4320 of 19284 results

  7. pGBW-m4046747

    Plasmid
    #149249
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149249-plasmid-map-sequence-id-358332

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  8. pGBW-m4046799

    Plasmid
    #145577
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145577-plasmid-map-sequence-id-292504

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  10. RANLS-IETD-BNES

    Plasmid
    #60972
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-8 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddRFP A and ddRFP B
    Tags
    NES sequence LALKLAGLDIGS and triplicated NLS seq…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pJCX4

    Plasmid
    #170756
    Purpose
    Express in mammalian cells a target protein C-terminally fused to the Mxe GryA intein - chitin binding domain (CBD) tag.
    Depositor
    Roberto Dominguez
    Article
    Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi
    Type
    Empty backbone
    Tags
    Mxe GyrA intein - chitin binding domain (CBD)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    170756-plasmid-map-sequence-id-334719

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Showing: 4301 - 4320 of 19284 results