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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,840 results for: codon optimized

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Showing: 4301 - 4320 of 4840 results

  4. pDIV300

    Plasmid
    #204968
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance marker
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    hph (hygromycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available Since
    Nov. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    204968-plasmid-map-sequence-id-454124

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  6. pNMSB99

    Plasmid
    #199308
    Purpose
    MosSCI insertion of myo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605
    Depositor
    Michael Krieg
    Article
    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4
    Insert
    myo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
    Expression
    Worm
    Mutation
    mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…
    Promoter
    myo-3
    Available Since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    199308-plasmid-map-sequence-id-404762

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  9. pCJ203

    Plasmid
    #162678
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaF2-A75; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162678-plasmid-map-sequence-id-321524

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  10. pGBW-m4133872

    Plasmid
    #152464
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available Since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152464-plasmid-map-sequence-id-309465

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  11. PECR

    Plasmid
    #39158
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PECR (PECR Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. 3xFLAG-dCas9/pMSCVpuro

    Plasmid
    #134983
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134983-plasmid-map-sequence-id-430623

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  14. pGBW-m4133862

    Plasmid
    #152487
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available Since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152487-plasmid-map-sequence-id-317734

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  15. pGBW-m4133568

    Plasmid
    #152340
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;V5
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available Since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152340-plasmid-map-sequence-id-420347

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  16. pGBW-m4133586

    Plasmid
    #152328
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHis-003
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available Since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152328-plasmid-map-sequence-id-342371

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  17. pJW1594

    Plasmid
    #121061
    Purpose
    TagRFP-T^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    TagRFP-T^SEC^BioTag::AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…
    Expression
    Worm
    Available Since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    121061-plasmid-map-sequence-id-235980

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Showing: 4301 - 4320 of 4840 results