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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,840 results for: donor

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Showing: 4301 - 4320 of 4840 results

  1. pMN-011

    Plasmid
    #195415
    Purpose
    AAVS1 Integratable mCherry Reporter for synZiFTR ZF3
    Depositor
    Ahmad Khalil
    Publication
    Li et al Science. 2022 Dec 16;378(6625):1227-1234
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    4X ZF3 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    Expression
    Mammalian
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195415-plasmid-map-sequence-id-384998

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  2. pMN-013

    Plasmid
    #195417
    Purpose
    AAVS1 Integratable mCherry Reporter for synZiFTR ZF5
    Depositor
    Ahmad Khalil
    Publication
    Li et al Science. 2022 Dec 16;378(6625):1227-1234
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    4X ZF5 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    Expression
    Mammalian
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195417-plasmid-map-sequence-id-385000

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  3. pPD687 A2UCOE-HBB_IVS2-EnAsCas12a-BFP

    Plasmid
    #249160
    Purpose
    Overexpression of EnAsCas12a-BFP with HBB IVS2 intron in 5' UTR with blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site.
    Depositor
    Michael Bassik
    Publication
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    A2UCOE-HBB_IVS2-EnAsCas12a-TagBFP (HBB Human, Synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249160-plasmid-map-sequence-id-496435

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  4. pSA073 HBB_IVS2-Cas9-BFP

    Plasmid
    #249134
    Purpose
    Overexpression of SpCas9-BFP with HBB IVS2 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Publication
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    HBB_IVS2-Cas9-TagBFP (HBB S. pyogenes Cas9, synthetic, Human)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249134-plasmid-map-sequence-id-496493

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  5. Hs.NRAS Q61K

    Plasmid
    #83180
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61K mutation
    Depositor
    Dominic Esposito
    Insert
    NRAS (NRAS Human)
    Use
    Gateway Cloning
    Mutation
    Q61K
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    83180-plasmid-map-sequence-id-158143

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  6. Hs.NRAS Q61R

    Plasmid
    #83179
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61R mutation
    Depositor
    Dominic Esposito
    Insert
    NRAS (NRAS Human)
    Use
    Gateway Cloning
    Mutation
    Q61R
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    83179-plasmid-map-sequence-id-158142

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  7. pSCL.180

    Plasmid
    #185001
    Purpose
    Express -Eco1 AAVS1 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Publication
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Eco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    AAVS1 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    185001-plasmid-map-sequence-id-376682

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  8. pSCL.177

    Plasmid
    #184998
    Purpose
    Express -Eco1 EMX1 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Publication
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Eco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    EMX1 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184998-plasmid-map-sequence-id-376623

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  9. pSCL.175

    Plasmid
    #184996
    Purpose
    Express -Eco1 HEK3 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Publication
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Eco1: HEK3 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    HEK3 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184996-plasmid-map-sequence-id-376621

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  10. ScEnSor Kit

    Plasmid kit
    #1000000215
    Purpose
    To investigate the intracellular environment of Saccharomyces cerevisiae with a variety of biosensors.
    Depositor
    Lisbeth Olsson
    Application
    Gene Expression and Labeling
    Vector type
    Yeast Expression
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only

  11. pODN-mNG-ECT2

    Plasmid
    #183835
    Purpose
    Repair template for the N-terminal tagging of Ect2 with mNeonGreen in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ECT2 homology arms with mNeonGreen-linker (ECT2 Human)
    Use
    CRISPR; Donor template
    Tags
    mNeonGreen-linker
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183835-plasmid-map-sequence-id-373473

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  12. R777-E039 Hs.DUSP4

    Plasmid
    #70323
    Purpose
    Gateway ORF clone of human DUSP4 [NM_001394.6] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    DUSP4 (DUSP4 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    70323-plasmid-map-sequence-id-125772

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  13. pODN-AAVS1-mR2-CAAX

    Plasmid
    #183871
    Purpose
    Repair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1 homology arms with CMV-driven mRuby2-CAAX fusion (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    mRuby2-CAAX
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    CMV
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183871-plasmid-map-sequence-id-373475

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  14. R777-E227 Hs.RASA1

    Plasmid
    #70511
    Purpose
    Gateway ORF clone of human RASA1 [NM_002890.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASA1 (RASA1 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    70511-plasmid-map-sequence-id-125960

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  16. pMH012_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

    Plasmid
    #179435
    Purpose
    Dual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Lacra Bintu
    Publication
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TRE-cit-coreHS4-mch (SC)
    Expression
    Mammalian
    Promoter
    pEF alpha, pRSV
    Available since
    Feb. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    179435-plasmid-map-sequence-id-355551

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  17. R777-E015 Hs.BRAF

    Plasmid
    #70299
    Purpose
    Gateway ORF clone of human BRAF [NM_004333.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    BRAF (BRAF Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    70299-plasmid-map-sequence-id-125748

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  18. pMH015_AAVS1_puro-pA-core-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

    Plasmid
    #179437
    Purpose
    Dual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Lacra Bintu
    Publication
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    coreHS4-TRE-cit-coreHS4-mch (DC)
    Expression
    Mammalian
    Promoter
    pEF alpha, pRSV
    Available since
    Feb. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    179437-plasmid-map-sequence-id-355553

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  19. Hs.KRAS4b Q61H

    Plasmid
    #83140
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61H mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway Cloning
    Mutation
    Q61H
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    83140-plasmid-map-sequence-id-158103

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  20. R777-E125 Hs.MAPK1

    Plasmid
    #70409
    Purpose
    Gateway ORF clone of human MAPK1 [NM_002745.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    MAPK1 (MAPK1 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    70409-plasmid-map-sequence-id-125858

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Showing: 4301 - 4320 of 4840 results