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ORF18(R74A)-3xFLAG pCDNA4

Plasmid

#129783

Purpose

Expresses KSHV ORF18 (R74A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Arginine 74 to Alanine

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(R180A)-3xFLAG pCDNA4

Plasmid

#129806

Purpose

Expresses KSHV ORF18 (R180A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Arginine 180 to Alanine

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(G214A)-3xFLAG pCDNA4

Plasmid

#129808

Purpose

Expresses KSHV ORF18 (G214A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Glycine 214 to Alanine

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18 (Y85A)-3xFLAG pCDNA4

Plasmid

#129786

Purpose

Expresses KSHV ORF18 (Y85A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Tyrosine 85 to Alanine

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(R74A_K75A)-3xFLAG pCDNA4

Plasmid

#129811

Purpose

Expresses KSHV ORF18 (R74A_K75A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Arginine 74 to Alanine and Lysine 75 to A…

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(W170A_G171A)-3xFLAG pCDNA4

Plasmid

#129815

Purpose

Expresses KSHV ORF18 (W170A_G171A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Tryptophan 170 to Alanine and Glycine 171…

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(R158A)-3xFLAG pCDNA4

Plasmid

#129801

Purpose

Expresses KSHV ORF18 (R158A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Arginine 158 to Alanine

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(G145A)-3xFLAG pCDNA4

Plasmid

#129796

Purpose

Expresses KSHV ORF18 (G145A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Glycine 145 to Alanine

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(N146A)-3xFLAG pCDNA4

Plasmid

#129798

Purpose

Expresses KSHV ORF18 (N146A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Asparagine 146 to Alanine

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-K295TAG-W340A.pcDNA3-k

Plasmid

#126627

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site.

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon and W34…

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/HP + GUG-nLuc-3XFLAG

Plasmid

#127323

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with hairpin in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUU-nLuc-3XFLAG

Plasmid

#127305

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUU start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AAA-nLuc-3XFLAG

Plasmid

#127306

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AAA start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.13/AUG(30)-FFLuc-3XFLAG

Plasmid

#127336

Purpose

Express truncated 3xFLAG tagged firefly luciferase (FFLuc) from AUG at codon 30

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

Firefly luciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

SV40

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AGG-nLuc-3XFLAG

Plasmid

#127304

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AGG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/ACG-nLuc-3XFLAG

Plasmid

#127303

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from ACG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GGG-nLuc-3XFLAG

Plasmid

#127307

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from GGG start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.13/AUG(59)-FFLuc-3XFLAG

Plasmid

#127337

Purpose

Express truncated 3xFLAG tagged firefly luciferase (FFLuc) from AUG at codon 59

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

Firefly luciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

SV40

Available Since

June 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RPS14(89-151)

Plasmid

#122299

Purpose

expresses a part of RPS14 protein with a flag tag

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RPS14 (RPS14 Human)

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 1 to 88

Promoter

CMV

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-Flag-natT1R2 d835

Plasmid

#113959

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

residues 22-834

Promoter

cmv

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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